Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25970

Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25965 and BWI76_RS25970 overlap by 4 nucleotidesBWI76_RS25970 and BWI76_RS25975 are separated by 35 nucleotidesBWI76_RS25975 and BWI76_RS25980 are separated by 46 nucleotides BWI76_RS25965: BWI76_RS25965 - hydrolase, at 5,368,197 to 5,369,219 _RS25965 BWI76_RS25970: BWI76_RS25970 - hypothetical protein, at 5,369,216 to 5,369,434 _RS25970 BWI76_RS25975: BWI76_RS25975 - phosphoribulokinase, at 5,369,470 to 5,370,339 _RS25975 BWI76_RS25980: BWI76_RS25980 - hypothetical protein, at 5,370,386 to 5,370,790 _RS25980 Position (kb) 5369 5370Strain fitness (log2 ratio) -2 -1 0 1at 5368.640 kb on - strand, within BWI76_RS25965at 5368.806 kb on + strand, within BWI76_RS25965at 5368.858 kb on + strand, within BWI76_RS25965at 5368.937 kb on - strand, within BWI76_RS25965at 5368.937 kb on - strand, within BWI76_RS25965at 5369.035 kb on - strand, within BWI76_RS25965at 5369.271 kb on - strand, within BWI76_RS25970at 5369.314 kb on - strand, within BWI76_RS25970at 5369.379 kb on + strand, within BWI76_RS25970at 5369.392 kb on - strand, within BWI76_RS25970at 5369.392 kb on - strand, within BWI76_RS25970at 5369.503 kb on + strandat 5369.503 kb on + strandat 5369.711 kb on - strand, within BWI76_RS25975at 5369.746 kb on - strand, within BWI76_RS25975at 5369.868 kb on - strand, within BWI76_RS25975at 5370.107 kb on + strand, within BWI76_RS25975at 5370.115 kb on - strand, within BWI76_RS25975

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
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5,368,640 - BWI76_RS25965 0.43 -0.0
5,368,806 + BWI76_RS25965 0.60 +0.9
5,368,858 + BWI76_RS25965 0.65 -0.5
5,368,937 - BWI76_RS25965 0.72 -0.7
5,368,937 - BWI76_RS25965 0.72 +0.4
5,369,035 - BWI76_RS25965 0.82 +0.2
5,369,271 - BWI76_RS25970 0.25 -2.4
5,369,314 - BWI76_RS25970 0.45 -1.7
5,369,379 + BWI76_RS25970 0.74 -0.1
5,369,392 - BWI76_RS25970 0.80 -0.4
5,369,392 - BWI76_RS25970 0.80 -0.3
5,369,503 + +0.9
5,369,503 + -0.5
5,369,711 - BWI76_RS25975 0.28 -0.5
5,369,746 - BWI76_RS25975 0.32 +1.2
5,369,868 - BWI76_RS25975 0.46 +0.0
5,370,107 + BWI76_RS25975 0.73 -1.5
5,370,115 - BWI76_RS25975 0.74 -1.8

Or see this region's nucleotide sequence