Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20515

Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20510 and BWI76_RS20515 are separated by 254 nucleotidesBWI76_RS20515 and BWI76_RS20520 overlap by 4 nucleotides BWI76_RS20510: BWI76_RS20510 - glucokinase, at 4,295,077 to 4,296,042 _RS20510 BWI76_RS20515: BWI76_RS20515 - ion channel protein, at 4,296,297 to 4,297,532 _RS20515 BWI76_RS20520: BWI76_RS20520 - indolepyruvate decarboxylase, at 4,297,529 to 4,299,190 _RS20520 Position (kb) 4296 4297 4298Strain fitness (log2 ratio) -2 -1 0 1 2at 4295.337 kb on + strand, within BWI76_RS20510at 4295.468 kb on - strand, within BWI76_RS20510at 4295.492 kb on - strand, within BWI76_RS20510at 4295.651 kb on + strand, within BWI76_RS20510at 4295.723 kb on + strand, within BWI76_RS20510at 4295.859 kb on + strand, within BWI76_RS20510at 4295.859 kb on - strand, within BWI76_RS20510at 4295.870 kb on + strand, within BWI76_RS20510at 4295.882 kb on + strand, within BWI76_RS20510at 4295.900 kb on - strand, within BWI76_RS20510at 4295.910 kb on - strand, within BWI76_RS20510at 4296.119 kb on + strandat 4296.127 kb on - strandat 4296.127 kb on - strandat 4296.213 kb on - strandat 4296.243 kb on + strandat 4296.272 kb on - strandat 4296.428 kb on + strand, within BWI76_RS20515at 4296.637 kb on + strandat 4296.645 kb on - strand, within BWI76_RS20515at 4296.779 kb on - strand, within BWI76_RS20515at 4296.955 kb on - strand, within BWI76_RS20515at 4297.144 kb on - strand, within BWI76_RS20515at 4297.176 kb on - strand, within BWI76_RS20515at 4297.311 kb on - strand, within BWI76_RS20515at 4297.557 kb on - strandat 4297.561 kb on + strandat 4297.626 kb on - strandat 4297.842 kb on - strand, within BWI76_RS20520at 4297.905 kb on + strand, within BWI76_RS20520at 4298.009 kb on - strand, within BWI76_RS20520at 4298.265 kb on - strand, within BWI76_RS20520at 4298.302 kb on + strand, within BWI76_RS20520at 4298.302 kb on + strand, within BWI76_RS20520

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
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4,295,337 + BWI76_RS20510 0.27 +2.4
4,295,468 - BWI76_RS20510 0.40 +0.6
4,295,492 - BWI76_RS20510 0.43 +0.4
4,295,651 + BWI76_RS20510 0.59 +0.6
4,295,723 + BWI76_RS20510 0.67 +0.1
4,295,859 + BWI76_RS20510 0.81 +1.4
4,295,859 - BWI76_RS20510 0.81 +1.2
4,295,870 + BWI76_RS20510 0.82 +0.9
4,295,882 + BWI76_RS20510 0.83 -0.7
4,295,900 - BWI76_RS20510 0.85 -2.3
4,295,910 - BWI76_RS20510 0.86 +0.9
4,296,119 + +0.8
4,296,127 - +0.8
4,296,127 - +0.2
4,296,213 - +0.6
4,296,243 + +1.4
4,296,272 - -1.7
4,296,428 + BWI76_RS20515 0.11 +0.9
4,296,637 + +1.6
4,296,645 - BWI76_RS20515 0.28 +1.0
4,296,779 - BWI76_RS20515 0.39 +2.2
4,296,955 - BWI76_RS20515 0.53 +0.8
4,297,144 - BWI76_RS20515 0.69 +0.6
4,297,176 - BWI76_RS20515 0.71 +0.7
4,297,311 - BWI76_RS20515 0.82 +0.3
4,297,557 - +0.3
4,297,561 + +0.6
4,297,626 - -0.2
4,297,842 - BWI76_RS20520 0.19 +0.2
4,297,905 + BWI76_RS20520 0.23 +1.3
4,298,009 - BWI76_RS20520 0.29 -0.8
4,298,265 - BWI76_RS20520 0.44 +1.6
4,298,302 + BWI76_RS20520 0.47 +1.0
4,298,302 + BWI76_RS20520 0.47 -1.0

Or see this region's nucleotide sequence