Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20135 and BWI76_RS20140 are separated by 157 nucleotides BWI76_RS20140 and BWI76_RS20145 overlap by 4 nucleotides BWI76_RS20145 and BWI76_RS20150 overlap by 4 nucleotides BWI76_RS20150 and BWI76_RS20155 are separated by 10 nucleotides
BWI76_RS20135: BWI76_RS20135 - NADH-quinone oxidoreductase subunit M, at 4,218,451 to 4,219,980
_RS20135
BWI76_RS20140: BWI76_RS20140 - NADH-quinone oxidoreductase subunit L, at 4,220,138 to 4,221,979
_RS20140
BWI76_RS20145: BWI76_RS20145 - NADH-quinone oxidoreductase subunit K, at 4,221,976 to 4,222,278
_RS20145
BWI76_RS20150: BWI76_RS20150 - NADH:ubiquinone oxidoreductase subunit J, at 4,222,275 to 4,222,829
_RS20150
BWI76_RS20155: BWI76_RS20155 - NADH-quinone oxidoreductase subunit I, at 4,222,840 to 4,223,382
_RS20155
Position (kb) 4220
4221
4222 Strain fitness (log2 ratio) -2
-1
0
1 at 4219.526 kb on + strand, within BWI76_RS20135 at 4219.829 kb on - strand at 4220.091 kb on - strand at 4220.202 kb on - strand at 4220.369 kb on - strand, within BWI76_RS20140 at 4220.380 kb on - strand, within BWI76_RS20140 at 4220.702 kb on - strand, within BWI76_RS20140 at 4221.166 kb on + strand, within BWI76_RS20140 at 4221.174 kb on - strand, within BWI76_RS20140 at 4221.317 kb on + strand, within BWI76_RS20140 at 4221.504 kb on + strand, within BWI76_RS20140 at 4221.544 kb on + strand, within BWI76_RS20140 at 4222.044 kb on + strand, within BWI76_RS20145 at 4222.323 kb on + strand at 4222.604 kb on + strand, within BWI76_RS20150 at 4222.645 kb on - strand, within BWI76_RS20150 at 4222.756 kb on + strand, within BWI76_RS20150 at 4222.891 kb on + strand at 4222.899 kb on - strand, within BWI76_RS20155
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days remove 4,219,526 + BWI76_RS20135 0.70 -0.2 4,219,829 - -0.8 4,220,091 - +1.3 4,220,202 - -1.7 4,220,369 - BWI76_RS20140 0.13 -2.1 4,220,380 - BWI76_RS20140 0.13 -2.7 4,220,702 - BWI76_RS20140 0.31 +0.6 4,221,166 + BWI76_RS20140 0.56 +0.3 4,221,174 - BWI76_RS20140 0.56 +0.4 4,221,317 + BWI76_RS20140 0.64 -1.9 4,221,504 + BWI76_RS20140 0.74 -1.1 4,221,544 + BWI76_RS20140 0.76 -1.0 4,222,044 + BWI76_RS20145 0.22 -0.6 4,222,323 + +0.1 4,222,604 + BWI76_RS20150 0.59 +0.1 4,222,645 - BWI76_RS20150 0.67 +1.5 4,222,756 + BWI76_RS20150 0.87 -1.3 4,222,891 + +1.4 4,222,899 - BWI76_RS20155 0.11 -1.1
Or see this region's nucleotide sequence