Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS18745

Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS18735 and BWI76_RS18740 are separated by 49 nucleotidesBWI76_RS18740 and BWI76_RS18745 are separated by 185 nucleotidesBWI76_RS18745 and BWI76_RS18750 are separated by 131 nucleotidesBWI76_RS18750 and BWI76_RS18755 are separated by 39 nucleotides BWI76_RS18735: BWI76_RS18735 - tautomerase family protein, at 3,884,275 to 3,884,664 _RS18735 BWI76_RS18740: BWI76_RS18740 - HPP family protein, at 3,884,714 to 3,885,445 _RS18740 BWI76_RS18745: BWI76_RS18745 - adenosylhomocysteinase, at 3,885,631 to 3,886,740 _RS18745 BWI76_RS18750: BWI76_RS18750 - adenosylcobinamide-GDP ribazoletransferase, at 3,886,872 to 3,887,612 _RS18750 BWI76_RS18755: BWI76_RS18755 - adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase, at 3,887,652 to 3,887,885 _RS18755 Position (kb) 3885 3886 3887Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3884.667 kb on - strandat 3884.688 kb on + strandat 3884.876 kb on - strand, within BWI76_RS18740at 3884.876 kb on - strand, within BWI76_RS18740at 3885.111 kb on + strand, within BWI76_RS18740at 3885.158 kb on + strand, within BWI76_RS18740at 3885.192 kb on + strand, within BWI76_RS18740at 3885.591 kb on - strandat 3885.673 kb on + strandat 3885.741 kb on - strandat 3885.838 kb on + strand, within BWI76_RS18745at 3886.136 kb on - strand, within BWI76_RS18745at 3886.851 kb on - strandat 3887.025 kb on + strand, within BWI76_RS18750at 3887.114 kb on - strand, within BWI76_RS18750at 3887.695 kb on + strand, within BWI76_RS18755

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
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3,884,667 - -0.4
3,884,688 + +0.4
3,884,876 - BWI76_RS18740 0.22 +2.1
3,884,876 - BWI76_RS18740 0.22 -3.4
3,885,111 + BWI76_RS18740 0.54 +2.0
3,885,158 + BWI76_RS18740 0.61 -0.4
3,885,192 + BWI76_RS18740 0.65 +0.8
3,885,591 - +0.6
3,885,673 + -0.1
3,885,741 - -2.3
3,885,838 + BWI76_RS18745 0.19 -2.5
3,886,136 - BWI76_RS18745 0.45 -0.2
3,886,851 - -0.6
3,887,025 + BWI76_RS18750 0.21 -1.0
3,887,114 - BWI76_RS18750 0.33 -1.2
3,887,695 + BWI76_RS18755 0.18 -0.6

Or see this region's nucleotide sequence