Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00590

Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00580 and BWI76_RS00585 are separated by 17 nucleotidesBWI76_RS00585 and BWI76_RS00590 are separated by 15 nucleotidesBWI76_RS00590 and BWI76_RS00595 overlap by 4 nucleotides BWI76_RS00580: BWI76_RS00580 - ribokinase, at 115,688 to 116,899 _RS00580 BWI76_RS00585: BWI76_RS00585 - dihydrodipicolinate synthase family protein, at 116,917 to 117,840 _RS00585 BWI76_RS00590: BWI76_RS00590 - hypothetical protein, at 117,856 to 118,347 _RS00590 BWI76_RS00595: BWI76_RS00595 - MFS transporter, at 118,344 to 119,621 _RS00595 Position (kb) 117 118 119Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 116.872 kb on + strandat 116.879 kb on + strandat 116.884 kb on + strandat 116.884 kb on + strandat 116.884 kb on + strandat 116.884 kb on + strandat 116.887 kb on - strandat 116.892 kb on - strandat 116.892 kb on - strandat 116.892 kb on - strandat 116.892 kb on - strandat 116.892 kb on - strandat 116.892 kb on - strandat 116.893 kb on + strandat 116.893 kb on + strandat 116.893 kb on + strandat 116.894 kb on - strandat 116.894 kb on - strandat 116.901 kb on - strandat 116.950 kb on - strandat 117.034 kb on - strand, within BWI76_RS00585at 117.034 kb on - strand, within BWI76_RS00585at 117.072 kb on - strand, within BWI76_RS00585at 117.077 kb on + strand, within BWI76_RS00585at 117.112 kb on - strand, within BWI76_RS00585at 117.115 kb on - strand, within BWI76_RS00585at 117.117 kb on - strand, within BWI76_RS00585at 117.215 kb on - strand, within BWI76_RS00585at 117.250 kb on - strand, within BWI76_RS00585at 117.285 kb on + strand, within BWI76_RS00585at 117.331 kb on + strandat 117.431 kb on + strand, within BWI76_RS00585at 117.444 kb on - strand, within BWI76_RS00585at 117.451 kb on - strand, within BWI76_RS00585at 117.488 kb on - strand, within BWI76_RS00585at 117.542 kb on + strand, within BWI76_RS00585at 117.564 kb on + strand, within BWI76_RS00585at 117.577 kb on + strand, within BWI76_RS00585at 117.795 kb on + strandat 117.801 kb on + strandat 117.829 kb on + strandat 117.859 kb on + strandat 117.891 kb on + strandat 117.899 kb on - strandat 118.113 kb on - strand, within BWI76_RS00590at 118.290 kb on - strand, within BWI76_RS00590at 118.448 kb on - strandat 118.450 kb on + strandat 118.458 kb on - strandat 118.524 kb on + strand, within BWI76_RS00595at 118.623 kb on + strand, within BWI76_RS00595at 118.631 kb on + strand, within BWI76_RS00595at 118.644 kb on - strand, within BWI76_RS00595at 118.757 kb on - strand, within BWI76_RS00595at 118.914 kb on + strand, within BWI76_RS00595at 118.951 kb on + strand, within BWI76_RS00595at 118.970 kb on - strand, within BWI76_RS00595at 118.999 kb on - strand, within BWI76_RS00595at 119.012 kb on + strand, within BWI76_RS00595at 119.120 kb on + strand, within BWI76_RS00595at 119.151 kb on - strand, within BWI76_RS00595at 119.242 kb on - strand, within BWI76_RS00595at 119.259 kb on - strand, within BWI76_RS00595at 119.274 kb on + strand, within BWI76_RS00595

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
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116,872 + +0.9
116,879 + -0.4
116,884 + -1.8
116,884 + +0.7
116,884 + +0.1
116,884 + +0.9
116,887 - -0.0
116,892 - +1.3
116,892 - -1.7
116,892 - -1.7
116,892 - +0.1
116,892 - +0.4
116,892 - +0.5
116,893 + +0.0
116,893 + +0.2
116,893 + -2.9
116,894 - -0.2
116,894 - +1.1
116,901 - +0.3
116,950 - +0.1
117,034 - BWI76_RS00585 0.13 +0.4
117,034 - BWI76_RS00585 0.13 +0.5
117,072 - BWI76_RS00585 0.17 +1.0
117,077 + BWI76_RS00585 0.17 +0.0
117,112 - BWI76_RS00585 0.21 -0.5
117,115 - BWI76_RS00585 0.21 +0.1
117,117 - BWI76_RS00585 0.22 -1.3
117,215 - BWI76_RS00585 0.32 -0.4
117,250 - BWI76_RS00585 0.36 +0.9
117,285 + BWI76_RS00585 0.40 +0.1
117,331 + +0.1
117,431 + BWI76_RS00585 0.56 +2.1
117,444 - BWI76_RS00585 0.57 -0.9
117,451 - BWI76_RS00585 0.58 -0.9
117,488 - BWI76_RS00585 0.62 -0.3
117,542 + BWI76_RS00585 0.68 +1.6
117,564 + BWI76_RS00585 0.70 +1.6
117,577 + BWI76_RS00585 0.71 -0.5
117,795 + +0.3
117,801 + -0.2
117,829 + +0.6
117,859 + -0.4
117,891 + +0.8
117,899 - -1.3
118,113 - BWI76_RS00590 0.52 -3.1
118,290 - BWI76_RS00590 0.88 -0.5
118,448 - -0.2
118,450 + +0.8
118,458 - -0.3
118,524 + BWI76_RS00595 0.14 +0.3
118,623 + BWI76_RS00595 0.22 +0.7
118,631 + BWI76_RS00595 0.22 +0.5
118,644 - BWI76_RS00595 0.23 -0.5
118,757 - BWI76_RS00595 0.32 +0.5
118,914 + BWI76_RS00595 0.45 +0.7
118,951 + BWI76_RS00595 0.47 +1.8
118,970 - BWI76_RS00595 0.49 +0.7
118,999 - BWI76_RS00595 0.51 +0.6
119,012 + BWI76_RS00595 0.52 +0.7
119,120 + BWI76_RS00595 0.61 -0.4
119,151 - BWI76_RS00595 0.63 +0.5
119,242 - BWI76_RS00595 0.70 +2.1
119,259 - BWI76_RS00595 0.72 +0.5
119,274 + BWI76_RS00595 0.73 +0.7

Or see this region's nucleotide sequence