Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS19625

Experiment: D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS19615 and BWI76_RS19620 are separated by 82 nucleotidesBWI76_RS19620 and BWI76_RS19625 overlap by 4 nucleotidesBWI76_RS19625 and BWI76_RS19630 are separated by 72 nucleotidesBWI76_RS19630 and BWI76_RS19635 are separated by 217 nucleotides BWI76_RS19615: BWI76_RS19615 - vancomycin high temperature exclusion protein, at 4,100,171 to 4,100,887 _RS19615 BWI76_RS19620: BWI76_RS19620 - hypothetical protein, at 4,100,970 to 4,101,629 _RS19620 BWI76_RS19625: BWI76_RS19625 - DUF4810 domain-containing protein, at 4,101,626 to 4,101,982 _RS19625 BWI76_RS19630: BWI76_RS19630 - putative lipoprotein, at 4,102,055 to 4,102,726 _RS19630 BWI76_RS19635: BWI76_RS19635 - galactoside ABC transporter permease MglC, at 4,102,944 to 4,103,954 _RS19635 Position (kb) 4101 4102Strain fitness (log2 ratio) -2 -1 0 1 2at 4100.665 kb on + strand, within BWI76_RS19615at 4100.830 kb on + strandat 4100.830 kb on + strandat 4100.830 kb on + strandat 4100.998 kb on - strandat 4101.014 kb on + strandat 4101.042 kb on - strand, within BWI76_RS19620at 4101.287 kb on + strand, within BWI76_RS19620at 4101.288 kb on - strand, within BWI76_RS19620at 4101.295 kb on - strand, within BWI76_RS19620at 4101.348 kb on + strand, within BWI76_RS19620at 4101.387 kb on + strand, within BWI76_RS19620at 4101.445 kb on - strand, within BWI76_RS19620at 4101.450 kb on - strand, within BWI76_RS19620at 4101.517 kb on - strand, within BWI76_RS19620at 4101.531 kb on - strand, within BWI76_RS19620at 4101.531 kb on - strand, within BWI76_RS19620at 4101.531 kb on - strand, within BWI76_RS19620at 4101.543 kb on + strand, within BWI76_RS19620at 4101.574 kb on - strandat 4101.576 kb on + strandat 4101.581 kb on + strandat 4101.581 kb on + strandat 4101.581 kb on + strandat 4101.582 kb on - strandat 4101.584 kb on - strandat 4101.589 kb on - strandat 4101.589 kb on - strandat 4101.589 kb on - strandat 4101.589 kb on - strandat 4101.589 kb on - strandat 4101.589 kb on - strandat 4101.602 kb on - strandat 4101.701 kb on - strand, within BWI76_RS19625at 4101.722 kb on - strand, within BWI76_RS19625at 4101.722 kb on - strand, within BWI76_RS19625at 4101.725 kb on + strand, within BWI76_RS19625at 4101.772 kb on + strand, within BWI76_RS19625at 4101.780 kb on - strand, within BWI76_RS19625at 4101.780 kb on - strand, within BWI76_RS19625at 4101.780 kb on - strand, within BWI76_RS19625at 4101.780 kb on - strand, within BWI76_RS19625at 4101.781 kb on + strand, within BWI76_RS19625at 4101.788 kb on - strand, within BWI76_RS19625at 4101.800 kb on - strand, within BWI76_RS19625at 4101.849 kb on + strand, within BWI76_RS19625at 4101.849 kb on + strandat 4101.849 kb on + strand, within BWI76_RS19625at 4101.849 kb on + strand, within BWI76_RS19625at 4101.851 kb on + strand, within BWI76_RS19625at 4101.857 kb on - strand, within BWI76_RS19625at 4101.857 kb on - strand, within BWI76_RS19625at 4101.857 kb on - strand, within BWI76_RS19625at 4101.857 kb on - strand, within BWI76_RS19625at 4101.857 kb on - strand, within BWI76_RS19625at 4101.861 kb on + strand, within BWI76_RS19625at 4101.861 kb on + strand, within BWI76_RS19625at 4101.861 kb on + strand, within BWI76_RS19625at 4101.876 kb on + strand, within BWI76_RS19625at 4101.900 kb on + strand, within BWI76_RS19625at 4101.907 kb on - strand, within BWI76_RS19625at 4101.908 kb on + strand, within BWI76_RS19625at 4101.908 kb on - strand, within BWI76_RS19625at 4101.909 kb on + strand, within BWI76_RS19625at 4101.910 kb on + strand, within BWI76_RS19625at 4101.910 kb on + strand, within BWI76_RS19625at 4101.910 kb on + strand, within BWI76_RS19625at 4101.910 kb on + strand, within BWI76_RS19625at 4101.910 kb on + strand, within BWI76_RS19625at 4101.910 kb on + strand, within BWI76_RS19625at 4101.910 kb on + strand, within BWI76_RS19625at 4101.910 kb on + strand, within BWI76_RS19625at 4101.910 kb on + strand, within BWI76_RS19625at 4101.910 kb on + strand, within BWI76_RS19625at 4101.910 kb on + strand, within BWI76_RS19625at 4101.910 kb on + strand, within BWI76_RS19625at 4101.910 kb on + strand, within BWI76_RS19625at 4101.910 kb on + strand, within BWI76_RS19625at 4101.910 kb on + strand, within BWI76_RS19625at 4101.910 kb on + strand, within BWI76_RS19625at 4101.910 kb on + strand, within BWI76_RS19625at 4101.910 kb on + strand, within BWI76_RS19625at 4101.910 kb on + strand, within BWI76_RS19625at 4101.910 kb on + strand, within BWI76_RS19625at 4101.910 kb on + strand, within BWI76_RS19625at 4101.910 kb on + strand, within BWI76_RS19625at 4101.916 kb on - strand, within BWI76_RS19625at 4101.917 kb on - strand, within BWI76_RS19625at 4101.917 kb on - strand, within BWI76_RS19625at 4101.917 kb on - strand, within BWI76_RS19625at 4101.917 kb on - strandat 4101.917 kb on - strand, within BWI76_RS19625at 4101.917 kb on - strand, within BWI76_RS19625at 4101.917 kb on - strand, within BWI76_RS19625at 4101.917 kb on - strand, within BWI76_RS19625at 4101.917 kb on - strand, within BWI76_RS19625at 4101.917 kb on - strand, within BWI76_RS19625at 4101.918 kb on - strand, within BWI76_RS19625at 4101.918 kb on - strand, within BWI76_RS19625at 4101.918 kb on - strand, within BWI76_RS19625at 4101.918 kb on - strand, within BWI76_RS19625at 4101.918 kb on - strand, within BWI76_RS19625at 4101.918 kb on - strand, within BWI76_RS19625at 4101.918 kb on - strandat 4101.918 kb on - strand, within BWI76_RS19625at 4101.918 kb on - strand, within BWI76_RS19625at 4101.918 kb on - strand, within BWI76_RS19625at 4101.918 kb on - strand, within BWI76_RS19625at 4101.918 kb on - strand, within BWI76_RS19625at 4101.918 kb on - strand, within BWI76_RS19625at 4101.918 kb on - strand, within BWI76_RS19625at 4101.918 kb on - strand, within BWI76_RS19625at 4101.918 kb on - strand, within BWI76_RS19625at 4101.918 kb on - strand, within BWI76_RS19625at 4101.918 kb on - strand, within BWI76_RS19625at 4101.918 kb on - strand, within BWI76_RS19625at 4101.918 kb on - strand, within BWI76_RS19625at 4102.012 kb on + strandat 4102.111 kb on - strandat 4102.111 kb on - strandat 4102.127 kb on + strand, within BWI76_RS19630at 4102.127 kb on + strand, within BWI76_RS19630at 4102.127 kb on + strand, within BWI76_RS19630at 4102.135 kb on - strand, within BWI76_RS19630at 4102.135 kb on - strand, within BWI76_RS19630at 4102.135 kb on - strand, within BWI76_RS19630at 4102.135 kb on - strand, within BWI76_RS19630at 4102.143 kb on - strand, within BWI76_RS19630at 4102.154 kb on + strand, within BWI76_RS19630at 4102.160 kb on - strand, within BWI76_RS19630at 4102.161 kb on - strand, within BWI76_RS19630at 4102.226 kb on - strand, within BWI76_RS19630at 4102.295 kb on - strand, within BWI76_RS19630at 4102.302 kb on + strand, within BWI76_RS19630at 4102.305 kb on - strand, within BWI76_RS19630at 4102.310 kb on - strandat 4102.368 kb on - strandat 4102.391 kb on - strandat 4102.396 kb on - strand, within BWI76_RS19630at 4102.408 kb on + strand, within BWI76_RS19630at 4102.408 kb on + strand, within BWI76_RS19630at 4102.408 kb on + strand, within BWI76_RS19630at 4102.408 kb on + strand, within BWI76_RS19630at 4102.408 kb on + strand, within BWI76_RS19630at 4102.408 kb on + strand, within BWI76_RS19630at 4102.408 kb on + strand, within BWI76_RS19630at 4102.416 kb on - strand, within BWI76_RS19630at 4102.416 kb on - strand, within BWI76_RS19630at 4102.416 kb on - strand, within BWI76_RS19630at 4102.416 kb on - strand, within BWI76_RS19630at 4102.427 kb on - strand, within BWI76_RS19630at 4102.440 kb on + strand, within BWI76_RS19630at 4102.448 kb on - strand, within BWI76_RS19630at 4102.448 kb on - strand, within BWI76_RS19630at 4102.448 kb on - strand, within BWI76_RS19630at 4102.448 kb on - strand, within BWI76_RS19630at 4102.468 kb on + strand, within BWI76_RS19630at 4102.572 kb on + strand, within BWI76_RS19630at 4102.614 kb on - strand, within BWI76_RS19630at 4102.619 kb on - strand, within BWI76_RS19630at 4102.620 kb on - strand, within BWI76_RS19630at 4102.623 kb on + strand, within BWI76_RS19630at 4102.623 kb on + strand, within BWI76_RS19630at 4102.623 kb on + strand, within BWI76_RS19630at 4102.623 kb on + strand, within BWI76_RS19630at 4102.630 kb on - strand, within BWI76_RS19630at 4102.631 kb on - strand, within BWI76_RS19630at 4102.631 kb on - strand, within BWI76_RS19630at 4102.631 kb on - strand, within BWI76_RS19630at 4102.933 kb on - strandat 4102.970 kb on + strandat 4102.979 kb on - strandat 4102.979 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days
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4,100,665 + BWI76_RS19615 0.69 -0.8
4,100,830 + -0.3
4,100,830 + +0.1
4,100,830 + -0.2
4,100,998 - +0.2
4,101,014 + -0.0
4,101,042 - BWI76_RS19620 0.11 +0.1
4,101,287 + BWI76_RS19620 0.48 +0.6
4,101,288 - BWI76_RS19620 0.48 -0.5
4,101,295 - BWI76_RS19620 0.49 -0.2
4,101,348 + BWI76_RS19620 0.57 +0.2
4,101,387 + BWI76_RS19620 0.63 -1.9
4,101,445 - BWI76_RS19620 0.72 +0.7
4,101,450 - BWI76_RS19620 0.73 +1.0
4,101,517 - BWI76_RS19620 0.83 +0.3
4,101,531 - BWI76_RS19620 0.85 +0.0
4,101,531 - BWI76_RS19620 0.85 -0.7
4,101,531 - BWI76_RS19620 0.85 -0.7
4,101,543 + BWI76_RS19620 0.87 -0.1
4,101,574 - +0.5
4,101,576 + +2.0
4,101,581 + -0.1
4,101,581 + -0.5
4,101,581 + -2.0
4,101,582 - +0.3
4,101,584 - +0.2
4,101,589 - -0.1
4,101,589 - +0.3
4,101,589 - +0.9
4,101,589 - -0.3
4,101,589 - +0.7
4,101,589 - +0.2
4,101,602 - -0.1
4,101,701 - BWI76_RS19625 0.21 -0.2
4,101,722 - BWI76_RS19625 0.27 -0.3
4,101,722 - BWI76_RS19625 0.27 +0.3
4,101,725 + BWI76_RS19625 0.28 -1.0
4,101,772 + BWI76_RS19625 0.41 -1.3
4,101,780 - BWI76_RS19625 0.43 +0.5
4,101,780 - BWI76_RS19625 0.43 +0.2
4,101,780 - BWI76_RS19625 0.43 +0.4
4,101,780 - BWI76_RS19625 0.43 -1.2
4,101,781 + BWI76_RS19625 0.43 -0.7
4,101,788 - BWI76_RS19625 0.45 +0.0
4,101,800 - BWI76_RS19625 0.49 +0.6
4,101,849 + BWI76_RS19625 0.62 -0.9
4,101,849 + +0.6
4,101,849 + BWI76_RS19625 0.62 +0.0
4,101,849 + BWI76_RS19625 0.62 -1.3
4,101,851 + BWI76_RS19625 0.63 -1.4
4,101,857 - BWI76_RS19625 0.65 -0.2
4,101,857 - BWI76_RS19625 0.65 -1.2
4,101,857 - BWI76_RS19625 0.65 -0.1
4,101,857 - BWI76_RS19625 0.65 -0.2
4,101,857 - BWI76_RS19625 0.65 -0.7
4,101,861 + BWI76_RS19625 0.66 +0.3
4,101,861 + BWI76_RS19625 0.66 -0.1
4,101,861 + BWI76_RS19625 0.66 -0.3
4,101,876 + BWI76_RS19625 0.70 -0.3
4,101,900 + BWI76_RS19625 0.77 +0.5
4,101,907 - BWI76_RS19625 0.79 -0.3
4,101,908 + BWI76_RS19625 0.79 -0.3
4,101,908 - BWI76_RS19625 0.79 +0.3
4,101,909 + BWI76_RS19625 0.79 -0.0
4,101,910 + BWI76_RS19625 0.80 -0.9
4,101,910 + BWI76_RS19625 0.80 -2.3
4,101,910 + BWI76_RS19625 0.80 -0.7
4,101,910 + BWI76_RS19625 0.80 -0.3
4,101,910 + BWI76_RS19625 0.80 -0.0
4,101,910 + BWI76_RS19625 0.80 +0.2
4,101,910 + BWI76_RS19625 0.80 -0.2
4,101,910 + BWI76_RS19625 0.80 +0.4
4,101,910 + BWI76_RS19625 0.80 -0.6
4,101,910 + BWI76_RS19625 0.80 -1.3
4,101,910 + BWI76_RS19625 0.80 -0.7
4,101,910 + BWI76_RS19625 0.80 -0.5
4,101,910 + BWI76_RS19625 0.80 -0.2
4,101,910 + BWI76_RS19625 0.80 +0.1
4,101,910 + BWI76_RS19625 0.80 -0.9
4,101,910 + BWI76_RS19625 0.80 -0.8
4,101,910 + BWI76_RS19625 0.80 -0.3
4,101,910 + BWI76_RS19625 0.80 -0.1
4,101,910 + BWI76_RS19625 0.80 +0.0
4,101,910 + BWI76_RS19625 0.80 -0.0
4,101,910 + BWI76_RS19625 0.80 +0.1
4,101,910 + BWI76_RS19625 0.80 +0.6
4,101,916 - BWI76_RS19625 0.81 -0.1
4,101,917 - BWI76_RS19625 0.82 +0.1
4,101,917 - BWI76_RS19625 0.82 +0.2
4,101,917 - BWI76_RS19625 0.82 +1.0
4,101,917 - -0.6
4,101,917 - BWI76_RS19625 0.82 +0.1
4,101,917 - BWI76_RS19625 0.82 -0.1
4,101,917 - BWI76_RS19625 0.82 -2.3
4,101,917 - BWI76_RS19625 0.82 +0.2
4,101,917 - BWI76_RS19625 0.82 -0.0
4,101,917 - BWI76_RS19625 0.82 -1.0
4,101,918 - BWI76_RS19625 0.82 +0.3
4,101,918 - BWI76_RS19625 0.82 -0.5
4,101,918 - BWI76_RS19625 0.82 +0.0
4,101,918 - BWI76_RS19625 0.82 -0.1
4,101,918 - BWI76_RS19625 0.82 -1.6
4,101,918 - BWI76_RS19625 0.82 -1.2
4,101,918 - -1.3
4,101,918 - BWI76_RS19625 0.82 +0.9
4,101,918 - BWI76_RS19625 0.82 +1.3
4,101,918 - BWI76_RS19625 0.82 -0.5
4,101,918 - BWI76_RS19625 0.82 +1.0
4,101,918 - BWI76_RS19625 0.82 -0.9
4,101,918 - BWI76_RS19625 0.82 +0.1
4,101,918 - BWI76_RS19625 0.82 -1.3
4,101,918 - BWI76_RS19625 0.82 -0.9
4,101,918 - BWI76_RS19625 0.82 +0.2
4,101,918 - BWI76_RS19625 0.82 -0.4
4,101,918 - BWI76_RS19625 0.82 -0.2
4,101,918 - BWI76_RS19625 0.82 -0.7
4,101,918 - BWI76_RS19625 0.82 -0.7
4,102,012 + +0.8
4,102,111 - +0.2
4,102,111 - -0.1
4,102,127 + BWI76_RS19630 0.11 +1.0
4,102,127 + BWI76_RS19630 0.11 +0.7
4,102,127 + BWI76_RS19630 0.11 +0.0
4,102,135 - BWI76_RS19630 0.12 +0.5
4,102,135 - BWI76_RS19630 0.12 +0.8
4,102,135 - BWI76_RS19630 0.12 +0.6
4,102,135 - BWI76_RS19630 0.12 +0.1
4,102,143 - BWI76_RS19630 0.13 +1.2
4,102,154 + BWI76_RS19630 0.15 -0.7
4,102,160 - BWI76_RS19630 0.16 +0.3
4,102,161 - BWI76_RS19630 0.16 -0.1
4,102,226 - BWI76_RS19630 0.25 +1.2
4,102,295 - BWI76_RS19630 0.36 +0.2
4,102,302 + BWI76_RS19630 0.37 +0.8
4,102,305 - BWI76_RS19630 0.37 +0.4
4,102,310 - -0.2
4,102,368 - +0.8
4,102,391 - -2.0
4,102,396 - BWI76_RS19630 0.51 +0.4
4,102,408 + BWI76_RS19630 0.53 -0.6
4,102,408 + BWI76_RS19630 0.53 -0.4
4,102,408 + BWI76_RS19630 0.53 +0.0
4,102,408 + BWI76_RS19630 0.53 -0.1
4,102,408 + BWI76_RS19630 0.53 +0.1
4,102,408 + BWI76_RS19630 0.53 +0.9
4,102,408 + BWI76_RS19630 0.53 -0.3
4,102,416 - BWI76_RS19630 0.54 -0.2
4,102,416 - BWI76_RS19630 0.54 +0.4
4,102,416 - BWI76_RS19630 0.54 +0.2
4,102,416 - BWI76_RS19630 0.54 -0.8
4,102,427 - BWI76_RS19630 0.55 +0.1
4,102,440 + BWI76_RS19630 0.57 +0.1
4,102,448 - BWI76_RS19630 0.58 +0.0
4,102,448 - BWI76_RS19630 0.58 +1.0
4,102,448 - BWI76_RS19630 0.58 -1.3
4,102,448 - BWI76_RS19630 0.58 +0.7
4,102,468 + BWI76_RS19630 0.61 +0.5
4,102,572 + BWI76_RS19630 0.77 -1.6
4,102,614 - BWI76_RS19630 0.83 -0.3
4,102,619 - BWI76_RS19630 0.84 +0.7
4,102,620 - BWI76_RS19630 0.84 -0.1
4,102,623 + BWI76_RS19630 0.85 -0.4
4,102,623 + BWI76_RS19630 0.85 +0.3
4,102,623 + BWI76_RS19630 0.85 +0.1
4,102,623 + BWI76_RS19630 0.85 -0.5
4,102,630 - BWI76_RS19630 0.86 -0.6
4,102,631 - BWI76_RS19630 0.86 +0.7
4,102,631 - BWI76_RS19630 0.86 +0.1
4,102,631 - BWI76_RS19630 0.86 -0.0
4,102,933 - -0.0
4,102,970 + +0.9
4,102,979 - -0.1
4,102,979 - +0.4

Or see this region's nucleotide sequence