Experiment: D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17555 and BWI76_RS17560 are separated by 136 nucleotides BWI76_RS17560 and BWI76_RS17565 are separated by 64 nucleotides BWI76_RS17565 and BWI76_RS17570 are separated by 65 nucleotides
BWI76_RS17555: BWI76_RS17555 - permease, at 3,643,666 to 3,645,138
_RS17555
BWI76_RS17560: BWI76_RS17560 - formate dehydrogenase subunit alpha, at 3,645,275 to 3,647,422
_RS17560
BWI76_RS17565: BWI76_RS17565 - hypothetical protein, at 3,647,487 to 3,648,092
_RS17565
BWI76_RS17570: BWI76_RS17570 - iron-sulfur-binding protein, at 3,648,158 to 3,648,616
_RS17570
Position (kb)
3645
3646
3647
3648 Strain fitness (log2 ratio)
-1
0
1 at 3644.314 kb on + strand, within BWI76_RS17555 at 3644.314 kb on + strand, within BWI76_RS17555 at 3644.324 kb on + strand, within BWI76_RS17555 at 3644.332 kb on - strand, within BWI76_RS17555 at 3644.332 kb on - strand, within BWI76_RS17555 at 3644.339 kb on + strand, within BWI76_RS17555 at 3644.659 kb on + strand, within BWI76_RS17555 at 3644.792 kb on + strand, within BWI76_RS17555 at 3644.826 kb on + strand, within BWI76_RS17555 at 3644.852 kb on + strand, within BWI76_RS17555 at 3645.060 kb on + strand at 3645.132 kb on - strand at 3645.251 kb on + strand at 3645.659 kb on - strand, within BWI76_RS17560 at 3645.950 kb on - strand, within BWI76_RS17560 at 3646.378 kb on + strand, within BWI76_RS17560 at 3646.378 kb on + strand, within BWI76_RS17560 at 3646.388 kb on + strand, within BWI76_RS17560 at 3646.633 kb on + strand, within BWI76_RS17560 at 3647.106 kb on + strand, within BWI76_RS17560 at 3647.446 kb on + strand at 3647.446 kb on + strand at 3647.446 kb on + strand at 3647.446 kb on + strand at 3647.447 kb on - strand at 3647.571 kb on - strand, within BWI76_RS17565 at 3647.834 kb on + strand, within BWI76_RS17565 at 3648.411 kb on - strand, within BWI76_RS17570
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days remove 3,644,314 + BWI76_RS17555 0.44 -0.6 3,644,314 + BWI76_RS17555 0.44 +0.4 3,644,324 + BWI76_RS17555 0.45 -0.8 3,644,332 - BWI76_RS17555 0.45 +1.6 3,644,332 - BWI76_RS17555 0.45 +0.6 3,644,339 + BWI76_RS17555 0.46 +0.5 3,644,659 + BWI76_RS17555 0.67 +0.2 3,644,792 + BWI76_RS17555 0.76 -0.1 3,644,826 + BWI76_RS17555 0.79 -1.1 3,644,852 + BWI76_RS17555 0.81 -0.2 3,645,060 + +0.2 3,645,132 - +0.8 3,645,251 + -0.6 3,645,659 - BWI76_RS17560 0.18 -0.0 3,645,950 - BWI76_RS17560 0.31 -0.0 3,646,378 + BWI76_RS17560 0.51 -0.3 3,646,378 + BWI76_RS17560 0.51 -0.5 3,646,388 + BWI76_RS17560 0.52 +0.7 3,646,633 + BWI76_RS17560 0.63 +1.5 3,647,106 + BWI76_RS17560 0.85 -0.2 3,647,446 + +0.2 3,647,446 + -0.5 3,647,446 + +0.2 3,647,446 + -0.5 3,647,447 - +0.1 3,647,571 - BWI76_RS17565 0.14 +0.5 3,647,834 + BWI76_RS17565 0.57 +0.3 3,648,411 - BWI76_RS17570 0.55 +0.0
Or see this region's nucleotide sequence