Experiment: D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16135 and BWI76_RS16140 are separated by 10 nucleotides BWI76_RS16140 and BWI76_RS16145 are separated by 10 nucleotides BWI76_RS16145 and BWI76_RS16150 are separated by 167 nucleotides BWI76_RS16150 and BWI76_RS16155 overlap by 4 nucleotides
BWI76_RS16135: BWI76_RS16135 - acetyl-CoA acetyltransferase, at 3,347,750 to 3,348,952
_RS16135
BWI76_RS16140: BWI76_RS16140 - 3-oxoadipate CoA-transferase, at 3,348,963 to 3,349,619
_RS16140
BWI76_RS16145: BWI76_RS16145 - 3-oxoadipate CoA-transferase, at 3,349,630 to 3,350,316
_RS16145
BWI76_RS16150: BWI76_RS16150 - IclR family transcriptional regulator, at 3,350,484 to 3,351,290
_RS16150
BWI76_RS16155: BWI76_RS16155 - hypothetical protein, at 3,351,287 to 3,351,850
_RS16155
Position (kb) 3349
3350
3351 Strain fitness (log2 ratio) -1
0
1 at 3349.004 kb on + strand at 3349.401 kb on - strand, within BWI76_RS16140 at 3349.411 kb on + strand, within BWI76_RS16140 at 3350.569 kb on + strand, within BWI76_RS16150 at 3350.577 kb on - strand, within BWI76_RS16150 at 3351.301 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days remove 3,349,004 + -0.9 3,349,401 - BWI76_RS16140 0.67 +0.8 3,349,411 + BWI76_RS16140 0.68 +0.1 3,350,569 + BWI76_RS16150 0.11 +0.0 3,350,577 - BWI76_RS16150 0.12 +0.6 3,351,301 - -0.6
Or see this region's nucleotide sequence