Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS24905

Experiment: D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS24895 and BWI76_RS24900 are separated by 110 nucleotidesBWI76_RS24900 and BWI76_RS24905 are separated by 64 nucleotidesBWI76_RS24905 and BWI76_RS24910 are separated by 4 nucleotidesBWI76_RS24910 and BWI76_RS24915 are separated by 63 nucleotides BWI76_RS24895: BWI76_RS24895 - galactitol-1-phosphate 5-dehydrogenase, at 5,187,513 to 5,188,556 _RS24895 BWI76_RS24900: BWI76_RS24900 - DeoR family transcriptional regulator, at 5,188,667 to 5,189,440 _RS24900 BWI76_RS24905: BWI76_RS24905 - filamentation induced by cAMP protein fic, at 5,189,505 to 5,190,506 _RS24905 BWI76_RS24910: BWI76_RS24910 - rRNA (cytidine-2'-O-)-methyltransferase, at 5,190,511 to 5,191,374 _RS24910 BWI76_RS24915: BWI76_RS24915 - penicillin-binding protein activator, at 5,191,438 to 5,193,519 _RS24915 Position (kb) 5189 5190 5191Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 5188.674 kb on + strandat 5188.833 kb on + strand, within BWI76_RS24900at 5188.991 kb on + strand, within BWI76_RS24900at 5189.060 kb on + strand, within BWI76_RS24900at 5189.135 kb on + strand, within BWI76_RS24900at 5189.410 kb on - strandat 5189.450 kb on - strandat 5189.470 kb on + strandat 5189.620 kb on + strand, within BWI76_RS24905at 5189.627 kb on - strand, within BWI76_RS24905at 5189.830 kb on - strand, within BWI76_RS24905at 5189.844 kb on - strand, within BWI76_RS24905at 5189.921 kb on + strand, within BWI76_RS24905at 5189.976 kb on + strand, within BWI76_RS24905at 5189.994 kb on + strand, within BWI76_RS24905at 5190.067 kb on + strand, within BWI76_RS24905at 5190.123 kb on + strand, within BWI76_RS24905at 5190.300 kb on - strand, within BWI76_RS24905at 5190.344 kb on - strand, within BWI76_RS24905at 5190.344 kb on - strand, within BWI76_RS24905at 5190.406 kb on + strandat 5190.410 kb on - strandat 5190.468 kb on + strandat 5190.481 kb on - strandat 5190.508 kb on + strandat 5190.517 kb on - strandat 5190.523 kb on - strandat 5190.536 kb on + strandat 5190.536 kb on + strandat 5190.536 kb on + strandat 5190.544 kb on - strandat 5190.544 kb on - strandat 5190.699 kb on - strand, within BWI76_RS24910at 5190.911 kb on - strand, within BWI76_RS24910at 5191.009 kb on + strand, within BWI76_RS24910at 5191.010 kb on + strand, within BWI76_RS24910at 5191.125 kb on - strand, within BWI76_RS24910at 5191.125 kb on - strand, within BWI76_RS24910at 5191.127 kb on + strand, within BWI76_RS24910at 5191.135 kb on - strand, within BWI76_RS24910at 5191.275 kb on + strand, within BWI76_RS24910at 5191.283 kb on - strand, within BWI76_RS24910at 5191.319 kb on - strandat 5191.336 kb on - strandat 5191.336 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days
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5,188,674 + +0.8
5,188,833 + BWI76_RS24900 0.21 -2.5
5,188,991 + BWI76_RS24900 0.42 -1.8
5,189,060 + BWI76_RS24900 0.51 -3.6
5,189,135 + BWI76_RS24900 0.60 -0.5
5,189,410 - +1.0
5,189,450 - -0.5
5,189,470 + -0.3
5,189,620 + BWI76_RS24905 0.11 +0.3
5,189,627 - BWI76_RS24905 0.12 +0.9
5,189,830 - BWI76_RS24905 0.32 +0.7
5,189,844 - BWI76_RS24905 0.34 +0.7
5,189,921 + BWI76_RS24905 0.42 +0.4
5,189,976 + BWI76_RS24905 0.47 -0.2
5,189,994 + BWI76_RS24905 0.49 +0.6
5,190,067 + BWI76_RS24905 0.56 +1.2
5,190,123 + BWI76_RS24905 0.62 +0.1
5,190,300 - BWI76_RS24905 0.79 +2.2
5,190,344 - BWI76_RS24905 0.84 +0.6
5,190,344 - BWI76_RS24905 0.84 -0.6
5,190,406 + +1.4
5,190,410 - +0.9
5,190,468 + +0.2
5,190,481 - -3.6
5,190,508 + +1.1
5,190,517 - +0.3
5,190,523 - +1.1
5,190,536 + +0.4
5,190,536 + -0.1
5,190,536 + +0.0
5,190,544 - +1.3
5,190,544 - +0.8
5,190,699 - BWI76_RS24910 0.22 -0.5
5,190,911 - BWI76_RS24910 0.46 -0.4
5,191,009 + BWI76_RS24910 0.58 -0.2
5,191,010 + BWI76_RS24910 0.58 -2.7
5,191,125 - BWI76_RS24910 0.71 +0.0
5,191,125 - BWI76_RS24910 0.71 -0.1
5,191,127 + BWI76_RS24910 0.71 +0.3
5,191,135 - BWI76_RS24910 0.72 +0.8
5,191,275 + BWI76_RS24910 0.88 -1.9
5,191,283 - BWI76_RS24910 0.89 +1.5
5,191,319 - +0.8
5,191,336 - +0.8
5,191,336 - -0.1

Or see this region's nucleotide sequence