Experiment: D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24455 and BWI76_RS24460 are separated by 58 nucleotides BWI76_RS24460 and BWI76_RS24465 are separated by 23 nucleotides
BWI76_RS24455: BWI76_RS24455 - bifunctional heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase, at 5,097,884 to 5,099,317
_RS24455
BWI76_RS24460: BWI76_RS24460 - bifunctional glutamine synthetase adenylyltransferase/deadenyltransferase, at 5,099,376 to 5,102,210
_RS24460
BWI76_RS24465: BWI76_RS24465 - adenylate cyclase, at 5,102,234 to 5,103,532
_RS24465
Position (kb) 5099
5100
5101
5102
5103 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 5099.498 kb on - strand at 5099.550 kb on - strand at 5099.550 kb on - strand at 5099.627 kb on - strand at 5099.872 kb on + strand, within BWI76_RS24460 at 5100.118 kb on + strand, within BWI76_RS24460 at 5100.281 kb on - strand, within BWI76_RS24460 at 5100.584 kb on + strand, within BWI76_RS24460 at 5100.735 kb on - strand, within BWI76_RS24460 at 5101.134 kb on + strand, within BWI76_RS24460 at 5101.472 kb on - strand, within BWI76_RS24460 at 5101.566 kb on + strand, within BWI76_RS24460 at 5101.612 kb on - strand, within BWI76_RS24460 at 5101.815 kb on - strand, within BWI76_RS24460 at 5101.862 kb on + strand, within BWI76_RS24460 at 5101.862 kb on + strand, within BWI76_RS24460 at 5101.862 kb on + strand, within BWI76_RS24460 at 5101.870 kb on - strand, within BWI76_RS24460 at 5101.870 kb on - strand, within BWI76_RS24460 at 5101.870 kb on - strand, within BWI76_RS24460 at 5102.171 kb on - strand at 5102.330 kb on + strand at 5102.359 kb on + strand at 5102.444 kb on - strand, within BWI76_RS24465 at 5102.514 kb on + strand, within BWI76_RS24465 at 5102.925 kb on + strand, within BWI76_RS24465 at 5102.996 kb on - strand, within BWI76_RS24465 at 5103.013 kb on + strand, within BWI76_RS24465 at 5103.021 kb on - strand, within BWI76_RS24465
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days remove 5,099,498 - -3.8 5,099,550 - -1.2 5,099,550 - +0.9 5,099,627 - -0.9 5,099,872 + BWI76_RS24460 0.17 -1.2 5,100,118 + BWI76_RS24460 0.26 -0.1 5,100,281 - BWI76_RS24460 0.32 +1.8 5,100,584 + BWI76_RS24460 0.43 -0.7 5,100,735 - BWI76_RS24460 0.48 -4.0 5,101,134 + BWI76_RS24460 0.62 -0.9 5,101,472 - BWI76_RS24460 0.74 +0.0 5,101,566 + BWI76_RS24460 0.77 -3.2 5,101,612 - BWI76_RS24460 0.79 -2.3 5,101,815 - BWI76_RS24460 0.86 -3.0 5,101,862 + BWI76_RS24460 0.88 +0.1 5,101,862 + BWI76_RS24460 0.88 +1.8 5,101,862 + BWI76_RS24460 0.88 -0.9 5,101,870 - BWI76_RS24460 0.88 -2.8 5,101,870 - BWI76_RS24460 0.88 -0.3 5,101,870 - BWI76_RS24460 0.88 +1.2 5,102,171 - +0.3 5,102,330 + +3.6 5,102,359 + +0.3 5,102,444 - BWI76_RS24465 0.16 -0.9 5,102,514 + BWI76_RS24465 0.22 -0.0 5,102,925 + BWI76_RS24465 0.53 +1.0 5,102,996 - BWI76_RS24465 0.59 +0.9 5,103,013 + BWI76_RS24465 0.60 -1.1 5,103,021 - BWI76_RS24465 0.61 +1.3
Or see this region's nucleotide sequence