Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS18900

Experiment: D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS18895 and BWI76_RS18900 overlap by 1 nucleotidesBWI76_RS18900 and BWI76_RS18905 are separated by 74 nucleotides BWI76_RS18895: BWI76_RS18895 - nitrogenase cofactor biosynthesis protein NifB, at 3,918,844 to 3,920,250 _RS18895 BWI76_RS18900: BWI76_RS18900 - protein NifQ, at 3,920,250 to 3,920,753 _RS18900 BWI76_RS18905: BWI76_RS18905 - putrescine/spermidine ABC transporter, at 3,920,828 to 3,922,186 _RS18905 Position (kb) 3920 3921Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 3919.431 kb on + strand, within BWI76_RS18895at 3919.431 kb on + strand, within BWI76_RS18895at 3919.550 kb on - strand, within BWI76_RS18895at 3920.014 kb on - strand, within BWI76_RS18895at 3920.081 kb on - strand, within BWI76_RS18895at 3920.334 kb on - strand, within BWI76_RS18900at 3920.451 kb on + strand, within BWI76_RS18900at 3920.565 kb on - strand, within BWI76_RS18900at 3920.846 kb on - strandat 3921.562 kb on - strand, within BWI76_RS18905

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days
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3,919,431 + BWI76_RS18895 0.42 -1.6
3,919,431 + BWI76_RS18895 0.42 -1.9
3,919,550 - BWI76_RS18895 0.50 -3.7
3,920,014 - BWI76_RS18895 0.83 -5.9
3,920,081 - BWI76_RS18895 0.88 -2.8
3,920,334 - BWI76_RS18900 0.17 -3.0
3,920,451 + BWI76_RS18900 0.40 -1.2
3,920,565 - BWI76_RS18900 0.62 -1.9
3,920,846 - +1.3
3,921,562 - BWI76_RS18905 0.54 +0.9

Or see this region's nucleotide sequence