Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS18890

Experiment: D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS18885 and BWI76_RS18890 overlap by 4 nucleotidesBWI76_RS18890 and BWI76_RS18895 are separated by 164 nucleotides BWI76_RS18885: BWI76_RS18885 - nitrogen fixation regulatory protein, at 3,915,621 to 3,917,108 _RS18885 BWI76_RS18890: BWI76_RS18890 - nif-specific transcriptional activator NifA, at 3,917,105 to 3,918,679 _RS18890 BWI76_RS18895: BWI76_RS18895 - nitrogenase cofactor biosynthesis protein NifB, at 3,918,844 to 3,920,250 _RS18895 Position (kb) 3917 3918 3919Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 3916.112 kb on + strand, within BWI76_RS18885at 3916.779 kb on - strand, within BWI76_RS18885at 3916.853 kb on - strand, within BWI76_RS18885at 3917.080 kb on + strandat 3917.098 kb on - strandat 3917.189 kb on + strandat 3917.189 kb on + strandat 3917.231 kb on + strandat 3917.256 kb on + strandat 3917.486 kb on - strand, within BWI76_RS18890at 3917.499 kb on - strand, within BWI76_RS18890at 3917.514 kb on + strand, within BWI76_RS18890at 3917.688 kb on - strand, within BWI76_RS18890at 3917.688 kb on - strand, within BWI76_RS18890at 3917.693 kb on - strand, within BWI76_RS18890at 3917.797 kb on - strand, within BWI76_RS18890at 3917.799 kb on + strand, within BWI76_RS18890at 3917.810 kb on - strand, within BWI76_RS18890at 3917.874 kb on - strand, within BWI76_RS18890at 3918.008 kb on - strand, within BWI76_RS18890at 3918.214 kb on - strand, within BWI76_RS18890at 3918.537 kb on + strandat 3918.592 kb on + strandat 3918.600 kb on - strandat 3918.820 kb on - strandat 3918.828 kb on + strandat 3918.879 kb on - strandat 3919.053 kb on + strand, within BWI76_RS18895at 3919.076 kb on - strand, within BWI76_RS18895at 3919.088 kb on - strand, within BWI76_RS18895at 3919.202 kb on + strand, within BWI76_RS18895at 3919.202 kb on + strand, within BWI76_RS18895at 3919.202 kb on + strand, within BWI76_RS18895at 3919.431 kb on + strand, within BWI76_RS18895at 3919.431 kb on + strand, within BWI76_RS18895at 3919.550 kb on - strand, within BWI76_RS18895

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days
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3,916,112 + BWI76_RS18885 0.33 -2.9
3,916,779 - BWI76_RS18885 0.78 -3.5
3,916,853 - BWI76_RS18885 0.83 -2.0
3,917,080 + -4.2
3,917,098 - -0.2
3,917,189 + -0.3
3,917,189 + -0.6
3,917,231 + +0.0
3,917,256 + -0.7
3,917,486 - BWI76_RS18890 0.24 -2.3
3,917,499 - BWI76_RS18890 0.25 -1.2
3,917,514 + BWI76_RS18890 0.26 -1.6
3,917,688 - BWI76_RS18890 0.37 -0.7
3,917,688 - BWI76_RS18890 0.37 -1.1
3,917,693 - BWI76_RS18890 0.37 -1.1
3,917,797 - BWI76_RS18890 0.44 -1.9
3,917,799 + BWI76_RS18890 0.44 -0.3
3,917,810 - BWI76_RS18890 0.45 -1.8
3,917,874 - BWI76_RS18890 0.49 -3.7
3,918,008 - BWI76_RS18890 0.57 -4.4
3,918,214 - BWI76_RS18890 0.70 -1.4
3,918,537 + -5.0
3,918,592 + -4.6
3,918,600 - -2.1
3,918,820 - -3.7
3,918,828 + -4.9
3,918,879 - -2.3
3,919,053 + BWI76_RS18895 0.15 -0.9
3,919,076 - BWI76_RS18895 0.16 -0.2
3,919,088 - BWI76_RS18895 0.17 -2.2
3,919,202 + BWI76_RS18895 0.25 -0.3
3,919,202 + BWI76_RS18895 0.25 -0.1
3,919,202 + BWI76_RS18895 0.25 -2.1
3,919,431 + BWI76_RS18895 0.42 -1.6
3,919,431 + BWI76_RS18895 0.42 -1.9
3,919,550 - BWI76_RS18895 0.50 -3.7

Or see this region's nucleotide sequence