Experiment: D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18885 and BWI76_RS18890 overlap by 4 nucleotides BWI76_RS18890 and BWI76_RS18895 are separated by 164 nucleotides
BWI76_RS18885: BWI76_RS18885 - nitrogen fixation regulatory protein, at 3,915,621 to 3,917,108
_RS18885
BWI76_RS18890: BWI76_RS18890 - nif-specific transcriptional activator NifA, at 3,917,105 to 3,918,679
_RS18890
BWI76_RS18895: BWI76_RS18895 - nitrogenase cofactor biosynthesis protein NifB, at 3,918,844 to 3,920,250
_RS18895
Position (kb)
3917
3918
3919 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3916.112 kb on + strand, within BWI76_RS18885 at 3916.779 kb on - strand, within BWI76_RS18885 at 3916.853 kb on - strand, within BWI76_RS18885 at 3917.080 kb on + strand at 3917.098 kb on - strand at 3917.189 kb on + strand at 3917.189 kb on + strand at 3917.231 kb on + strand at 3917.256 kb on + strand at 3917.486 kb on - strand, within BWI76_RS18890 at 3917.499 kb on - strand, within BWI76_RS18890 at 3917.514 kb on + strand, within BWI76_RS18890 at 3917.688 kb on - strand, within BWI76_RS18890 at 3917.688 kb on - strand, within BWI76_RS18890 at 3917.693 kb on - strand, within BWI76_RS18890 at 3917.797 kb on - strand, within BWI76_RS18890 at 3917.799 kb on + strand, within BWI76_RS18890 at 3917.810 kb on - strand, within BWI76_RS18890 at 3917.874 kb on - strand, within BWI76_RS18890 at 3918.008 kb on - strand, within BWI76_RS18890 at 3918.214 kb on - strand, within BWI76_RS18890 at 3918.537 kb on + strand at 3918.592 kb on + strand at 3918.600 kb on - strand at 3918.820 kb on - strand at 3918.828 kb on + strand at 3918.879 kb on - strand at 3919.053 kb on + strand, within BWI76_RS18895 at 3919.076 kb on - strand, within BWI76_RS18895 at 3919.088 kb on - strand, within BWI76_RS18895 at 3919.202 kb on + strand, within BWI76_RS18895 at 3919.202 kb on + strand, within BWI76_RS18895 at 3919.202 kb on + strand, within BWI76_RS18895 at 3919.431 kb on + strand, within BWI76_RS18895 at 3919.431 kb on + strand, within BWI76_RS18895 at 3919.550 kb on - strand, within BWI76_RS18895
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days remove 3,916,112 + BWI76_RS18885 0.33 -2.9 3,916,779 - BWI76_RS18885 0.78 -3.5 3,916,853 - BWI76_RS18885 0.83 -2.0 3,917,080 + -4.2 3,917,098 - -0.2 3,917,189 + -0.3 3,917,189 + -0.6 3,917,231 + +0.0 3,917,256 + -0.7 3,917,486 - BWI76_RS18890 0.24 -2.3 3,917,499 - BWI76_RS18890 0.25 -1.2 3,917,514 + BWI76_RS18890 0.26 -1.6 3,917,688 - BWI76_RS18890 0.37 -0.7 3,917,688 - BWI76_RS18890 0.37 -1.1 3,917,693 - BWI76_RS18890 0.37 -1.1 3,917,797 - BWI76_RS18890 0.44 -1.9 3,917,799 + BWI76_RS18890 0.44 -0.3 3,917,810 - BWI76_RS18890 0.45 -1.8 3,917,874 - BWI76_RS18890 0.49 -3.7 3,918,008 - BWI76_RS18890 0.57 -4.4 3,918,214 - BWI76_RS18890 0.70 -1.4 3,918,537 + -5.0 3,918,592 + -4.6 3,918,600 - -2.1 3,918,820 - -3.7 3,918,828 + -4.9 3,918,879 - -2.3 3,919,053 + BWI76_RS18895 0.15 -0.9 3,919,076 - BWI76_RS18895 0.16 -0.2 3,919,088 - BWI76_RS18895 0.17 -2.2 3,919,202 + BWI76_RS18895 0.25 -0.3 3,919,202 + BWI76_RS18895 0.25 -0.1 3,919,202 + BWI76_RS18895 0.25 -2.1 3,919,431 + BWI76_RS18895 0.42 -1.6 3,919,431 + BWI76_RS18895 0.42 -1.9 3,919,550 - BWI76_RS18895 0.50 -3.7
Or see this region's nucleotide sequence