Experiment: D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18835 and BWI76_RS18840 are separated by 10 nucleotides BWI76_RS18840 and BWI76_RS18845 overlap by 14 nucleotides BWI76_RS18845 and BWI76_RS18850 are separated by 195 nucleotides
BWI76_RS18835: BWI76_RS18835 - nitrogenase iron-molybdenum cofactor biosynthesis protein NifE, at 3,906,609 to 3,907,982
_RS18835
BWI76_RS18840: BWI76_RS18840 - nitrogenase iron-molybdenum cofactor biosynthesis protein NifN, at 3,907,993 to 3,909,378
_RS18840
BWI76_RS18845: BWI76_RS18845 - nitrogen fixation protein NifX, at 3,909,365 to 3,909,835
_RS18845
BWI76_RS18850: BWI76_RS18850 - iron-sulfur cluster assembly scaffold protein NifU, at 3,910,031 to 3,910,855
_RS18850
Position (kb)
3907
3908
3909
3910 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3907.024 kb on - strand, within BWI76_RS18835 at 3907.049 kb on + strand, within BWI76_RS18835 at 3907.394 kb on - strand, within BWI76_RS18835 at 3907.405 kb on - strand, within BWI76_RS18835 at 3907.984 kb on - strand at 3908.111 kb on - strand at 3908.286 kb on + strand, within BWI76_RS18840 at 3908.294 kb on - strand, within BWI76_RS18840 at 3908.372 kb on - strand, within BWI76_RS18840 at 3908.437 kb on + strand, within BWI76_RS18840 at 3908.437 kb on + strand, within BWI76_RS18840 at 3908.437 kb on + strand, within BWI76_RS18840 at 3908.445 kb on - strand, within BWI76_RS18840 at 3908.445 kb on - strand, within BWI76_RS18840 at 3908.445 kb on - strand, within BWI76_RS18840 at 3908.445 kb on - strand, within BWI76_RS18840 at 3908.445 kb on - strand, within BWI76_RS18840 at 3908.660 kb on - strand, within BWI76_RS18840 at 3908.681 kb on - strand, within BWI76_RS18840 at 3908.698 kb on + strand, within BWI76_RS18840 at 3908.706 kb on - strand, within BWI76_RS18840 at 3908.716 kb on - strand, within BWI76_RS18840 at 3908.798 kb on + strand, within BWI76_RS18840 at 3908.909 kb on + strand, within BWI76_RS18840 at 3908.917 kb on - strand, within BWI76_RS18840 at 3908.981 kb on - strand, within BWI76_RS18840 at 3909.008 kb on + strand, within BWI76_RS18840 at 3909.394 kb on + strand at 3909.471 kb on - strand at 3909.538 kb on - strand, within BWI76_RS18845 at 3909.610 kb on + strand, within BWI76_RS18845 at 3909.735 kb on + strand, within BWI76_RS18845 at 3910.023 kb on - strand at 3910.273 kb on + strand, within BWI76_RS18850 at 3910.355 kb on - strand, within BWI76_RS18850
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days remove 3,907,024 - BWI76_RS18835 0.30 -1.7 3,907,049 + BWI76_RS18835 0.32 -2.6 3,907,394 - BWI76_RS18835 0.57 -1.4 3,907,405 - BWI76_RS18835 0.58 -3.3 3,907,984 - +0.0 3,908,111 - -1.9 3,908,286 + BWI76_RS18840 0.21 -2.0 3,908,294 - BWI76_RS18840 0.22 -4.1 3,908,372 - BWI76_RS18840 0.27 -0.3 3,908,437 + BWI76_RS18840 0.32 -5.0 3,908,437 + BWI76_RS18840 0.32 -2.5 3,908,437 + BWI76_RS18840 0.32 -2.8 3,908,445 - BWI76_RS18840 0.33 -3.4 3,908,445 - BWI76_RS18840 0.33 -3.3 3,908,445 - BWI76_RS18840 0.33 -2.3 3,908,445 - BWI76_RS18840 0.33 -0.2 3,908,445 - BWI76_RS18840 0.33 -2.3 3,908,660 - BWI76_RS18840 0.48 -0.5 3,908,681 - BWI76_RS18840 0.50 -0.9 3,908,698 + BWI76_RS18840 0.51 -1.1 3,908,706 - BWI76_RS18840 0.51 -3.7 3,908,716 - BWI76_RS18840 0.52 -1.4 3,908,798 + BWI76_RS18840 0.58 -3.3 3,908,909 + BWI76_RS18840 0.66 -4.0 3,908,917 - BWI76_RS18840 0.67 -4.3 3,908,981 - BWI76_RS18840 0.71 +0.0 3,909,008 + BWI76_RS18840 0.73 -0.3 3,909,394 + +0.0 3,909,471 - -1.7 3,909,538 - BWI76_RS18845 0.37 +1.7 3,909,610 + BWI76_RS18845 0.52 -0.3 3,909,735 + BWI76_RS18845 0.79 +1.2 3,910,023 - -0.1 3,910,273 + BWI76_RS18850 0.29 -3.3 3,910,355 - BWI76_RS18850 0.39 -0.6
Or see this region's nucleotide sequence