Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS18840

Experiment: D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS18835 and BWI76_RS18840 are separated by 10 nucleotidesBWI76_RS18840 and BWI76_RS18845 overlap by 14 nucleotidesBWI76_RS18845 and BWI76_RS18850 are separated by 195 nucleotides BWI76_RS18835: BWI76_RS18835 - nitrogenase iron-molybdenum cofactor biosynthesis protein NifE, at 3,906,609 to 3,907,982 _RS18835 BWI76_RS18840: BWI76_RS18840 - nitrogenase iron-molybdenum cofactor biosynthesis protein NifN, at 3,907,993 to 3,909,378 _RS18840 BWI76_RS18845: BWI76_RS18845 - nitrogen fixation protein NifX, at 3,909,365 to 3,909,835 _RS18845 BWI76_RS18850: BWI76_RS18850 - iron-sulfur cluster assembly scaffold protein NifU, at 3,910,031 to 3,910,855 _RS18850 Position (kb) 3907 3908 3909 3910Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 3907.024 kb on - strand, within BWI76_RS18835at 3907.049 kb on + strand, within BWI76_RS18835at 3907.394 kb on - strand, within BWI76_RS18835at 3907.405 kb on - strand, within BWI76_RS18835at 3907.984 kb on - strandat 3908.111 kb on - strandat 3908.286 kb on + strand, within BWI76_RS18840at 3908.294 kb on - strand, within BWI76_RS18840at 3908.372 kb on - strand, within BWI76_RS18840at 3908.437 kb on + strand, within BWI76_RS18840at 3908.437 kb on + strand, within BWI76_RS18840at 3908.437 kb on + strand, within BWI76_RS18840at 3908.445 kb on - strand, within BWI76_RS18840at 3908.445 kb on - strand, within BWI76_RS18840at 3908.445 kb on - strand, within BWI76_RS18840at 3908.445 kb on - strand, within BWI76_RS18840at 3908.445 kb on - strand, within BWI76_RS18840at 3908.660 kb on - strand, within BWI76_RS18840at 3908.681 kb on - strand, within BWI76_RS18840at 3908.698 kb on + strand, within BWI76_RS18840at 3908.706 kb on - strand, within BWI76_RS18840at 3908.716 kb on - strand, within BWI76_RS18840at 3908.798 kb on + strand, within BWI76_RS18840at 3908.909 kb on + strand, within BWI76_RS18840at 3908.917 kb on - strand, within BWI76_RS18840at 3908.981 kb on - strand, within BWI76_RS18840at 3909.008 kb on + strand, within BWI76_RS18840at 3909.394 kb on + strandat 3909.471 kb on - strandat 3909.538 kb on - strand, within BWI76_RS18845at 3909.610 kb on + strand, within BWI76_RS18845at 3909.735 kb on + strand, within BWI76_RS18845at 3910.023 kb on - strandat 3910.273 kb on + strand, within BWI76_RS18850at 3910.355 kb on - strand, within BWI76_RS18850

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days
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3,907,024 - BWI76_RS18835 0.30 -1.7
3,907,049 + BWI76_RS18835 0.32 -2.6
3,907,394 - BWI76_RS18835 0.57 -1.4
3,907,405 - BWI76_RS18835 0.58 -3.3
3,907,984 - +0.0
3,908,111 - -1.9
3,908,286 + BWI76_RS18840 0.21 -2.0
3,908,294 - BWI76_RS18840 0.22 -4.1
3,908,372 - BWI76_RS18840 0.27 -0.3
3,908,437 + BWI76_RS18840 0.32 -5.0
3,908,437 + BWI76_RS18840 0.32 -2.5
3,908,437 + BWI76_RS18840 0.32 -2.8
3,908,445 - BWI76_RS18840 0.33 -3.4
3,908,445 - BWI76_RS18840 0.33 -3.3
3,908,445 - BWI76_RS18840 0.33 -2.3
3,908,445 - BWI76_RS18840 0.33 -0.2
3,908,445 - BWI76_RS18840 0.33 -2.3
3,908,660 - BWI76_RS18840 0.48 -0.5
3,908,681 - BWI76_RS18840 0.50 -0.9
3,908,698 + BWI76_RS18840 0.51 -1.1
3,908,706 - BWI76_RS18840 0.51 -3.7
3,908,716 - BWI76_RS18840 0.52 -1.4
3,908,798 + BWI76_RS18840 0.58 -3.3
3,908,909 + BWI76_RS18840 0.66 -4.0
3,908,917 - BWI76_RS18840 0.67 -4.3
3,908,981 - BWI76_RS18840 0.71 +0.0
3,909,008 + BWI76_RS18840 0.73 -0.3
3,909,394 + +0.0
3,909,471 - -1.7
3,909,538 - BWI76_RS18845 0.37 +1.7
3,909,610 + BWI76_RS18845 0.52 -0.3
3,909,735 + BWI76_RS18845 0.79 +1.2
3,910,023 - -0.1
3,910,273 + BWI76_RS18850 0.29 -3.3
3,910,355 - BWI76_RS18850 0.39 -0.6

Or see this region's nucleotide sequence