Experiment: D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16915 and BWI76_RS16920 are separated by 17 nucleotides BWI76_RS16920 and BWI76_RS16925 are separated by 1 nucleotides BWI76_RS16925 and BWI76_RS16930 are separated by 190 nucleotides
BWI76_RS16915: BWI76_RS16915 - alpha/beta hydrolase fold protein, at 3,507,370 to 3,508,236
_RS16915
BWI76_RS16920: BWI76_RS16920 - 3-(2,3-dihydroxyphenyl)propionate dioxygenase, at 3,508,254 to 3,509,198
_RS16920
BWI76_RS16925: BWI76_RS16925 - 3-(3-hydroxyphenyl)propionate hydroxylase, at 3,509,200 to 3,510,867
_RS16925
BWI76_RS16930: BWI76_RS16930 - transcriptional regulator, at 3,511,058 to 3,511,864
_RS16930
Position (kb)
3509
3510
3511 Strain fitness (log2 ratio)
-1
0
1
2 at 3508.418 kb on + strand, within BWI76_RS16920 at 3508.568 kb on + strand, within BWI76_RS16920 at 3509.124 kb on + strand at 3509.955 kb on - strand, within BWI76_RS16925 at 3510.070 kb on - strand, within BWI76_RS16925 at 3510.179 kb on - strand, within BWI76_RS16925 at 3510.192 kb on + strand, within BWI76_RS16925 at 3510.497 kb on - strand, within BWI76_RS16925 at 3510.514 kb on - strand, within BWI76_RS16925 at 3510.662 kb on - strand, within BWI76_RS16925 at 3510.824 kb on - strand at 3510.881 kb on + strand at 3510.966 kb on + strand at 3510.966 kb on + strand at 3511.275 kb on + strand, within BWI76_RS16930 at 3511.732 kb on - strand, within BWI76_RS16930 at 3511.754 kb on + strand, within BWI76_RS16930
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days remove 3,508,418 + BWI76_RS16920 0.17 +2.1 3,508,568 + BWI76_RS16920 0.33 -0.9 3,509,124 + -0.9 3,509,955 - BWI76_RS16925 0.45 -0.0 3,510,070 - BWI76_RS16925 0.52 +2.5 3,510,179 - BWI76_RS16925 0.59 +1.1 3,510,192 + BWI76_RS16925 0.59 +1.2 3,510,497 - BWI76_RS16925 0.78 +2.4 3,510,514 - BWI76_RS16925 0.79 +0.4 3,510,662 - BWI76_RS16925 0.88 +0.6 3,510,824 - +0.8 3,510,881 + +0.6 3,510,966 + +1.1 3,510,966 + +0.7 3,511,275 + BWI76_RS16930 0.27 +0.4 3,511,732 - BWI76_RS16930 0.84 -0.2 3,511,754 + BWI76_RS16930 0.86 +0.9
Or see this region's nucleotide sequence