Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS13400

Experiment: D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS13395 and BWI76_RS13400 are separated by 326 nucleotidesBWI76_RS13400 and BWI76_RS13405 are separated by 365 nucleotides BWI76_RS13395: BWI76_RS13395 - cytochrome D ubiquinol oxidase subunit I, at 2,792,601 to 2,793,998 _RS13395 BWI76_RS13400: BWI76_RS13400 - GntR family transcriptional regulator, at 2,794,325 to 2,795,737 _RS13400 BWI76_RS13405: BWI76_RS13405 - spermidine/putrescine ABC transporter substrate-binding protein, at 2,796,103 to 2,797,248 _RS13405 Position (kb) 2794 2795 2796Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2793.425 kb on - strand, within BWI76_RS13395at 2793.509 kb on - strand, within BWI76_RS13395at 2793.623 kb on - strand, within BWI76_RS13395at 2793.650 kb on + strand, within BWI76_RS13395at 2794.031 kb on + strandat 2794.147 kb on - strandat 2794.252 kb on - strandat 2794.381 kb on + strandat 2794.722 kb on - strand, within BWI76_RS13400at 2795.088 kb on + strand, within BWI76_RS13400at 2795.096 kb on - strand, within BWI76_RS13400at 2795.389 kb on + strand, within BWI76_RS13400at 2795.401 kb on + strand, within BWI76_RS13400at 2795.601 kb on - strandat 2795.663 kb on + strandat 2795.736 kb on + strandat 2795.751 kb on + strandat 2795.756 kb on + strandat 2795.772 kb on + strandat 2795.783 kb on + strandat 2796.023 kb on - strandat 2796.092 kb on + strandat 2796.141 kb on + strandat 2796.362 kb on + strand, within BWI76_RS13405at 2796.370 kb on - strand, within BWI76_RS13405at 2796.510 kb on + strand, within BWI76_RS13405

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days
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2,793,425 - BWI76_RS13395 0.59 -3.0
2,793,509 - BWI76_RS13395 0.65 +0.3
2,793,623 - BWI76_RS13395 0.73 -2.4
2,793,650 + BWI76_RS13395 0.75 -0.1
2,794,031 + +0.8
2,794,147 - -0.7
2,794,252 - +1.0
2,794,381 + +1.9
2,794,722 - BWI76_RS13400 0.28 +0.8
2,795,088 + BWI76_RS13400 0.54 +0.5
2,795,096 - BWI76_RS13400 0.55 +1.3
2,795,389 + BWI76_RS13400 0.75 +2.0
2,795,401 + BWI76_RS13400 0.76 +2.4
2,795,601 - +2.0
2,795,663 + +2.1
2,795,736 + +0.4
2,795,751 + +1.0
2,795,756 + -0.3
2,795,772 + +0.7
2,795,783 + +0.1
2,796,023 - -0.2
2,796,092 + -1.9
2,796,141 + +0.8
2,796,362 + BWI76_RS13405 0.23 -0.2
2,796,370 - BWI76_RS13405 0.23 -2.3
2,796,510 + BWI76_RS13405 0.36 +0.5

Or see this region's nucleotide sequence