Experiment: D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11130 and BWI76_RS11135 are separated by 102 nucleotides BWI76_RS11135 and BWI76_RS11140 are separated by 103 nucleotides BWI76_RS11140 and BWI76_RS11145 overlap by 2 nucleotides BWI76_RS11145 and BWI76_RS11150 are separated by 5 nucleotides
BWI76_RS11130: BWI76_RS11130 - PTS glucose transporter subunit IIBC, at 2,346,561 to 2,347,994
_RS11130
BWI76_RS11135: BWI76_RS11135 - ferric-rhodotorulic acid/ferric-coprogen receptor FhuE, at 2,348,097 to 2,350,283
_RS11135
BWI76_RS11140: BWI76_RS11140 - hypothetical protein, at 2,350,387 to 2,350,635
_RS11140
BWI76_RS11145: BWI76_RS11145 - histidine triad nucleotide-binding protein, at 2,350,634 to 2,350,990
_RS11145
BWI76_RS11150: BWI76_RS11150 - membrane protein, at 2,350,996 to 2,351,370
_RS11150
Position (kb)
2348
2349
2350
2351 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2347.171 kb on + strand, within BWI76_RS11130 at 2347.272 kb on - strand, within BWI76_RS11130 at 2347.279 kb on + strand, within BWI76_RS11130 at 2347.442 kb on - strand, within BWI76_RS11130 at 2347.518 kb on + strand, within BWI76_RS11130 at 2347.526 kb on - strand, within BWI76_RS11130 at 2347.736 kb on - strand, within BWI76_RS11130 at 2348.199 kb on - strand at 2348.210 kb on - strand at 2348.255 kb on + strand at 2348.408 kb on + strand, within BWI76_RS11135 at 2348.416 kb on - strand, within BWI76_RS11135 at 2348.416 kb on - strand, within BWI76_RS11135 at 2348.467 kb on - strand, within BWI76_RS11135 at 2348.748 kb on + strand, within BWI76_RS11135 at 2348.765 kb on - strand, within BWI76_RS11135 at 2349.072 kb on + strand, within BWI76_RS11135 at 2349.085 kb on - strand, within BWI76_RS11135 at 2349.811 kb on + strand, within BWI76_RS11135 at 2349.968 kb on + strand, within BWI76_RS11135 at 2351.192 kb on - strand, within BWI76_RS11150
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days remove 2,347,171 + BWI76_RS11130 0.43 -0.2 2,347,272 - BWI76_RS11130 0.50 -1.2 2,347,279 + BWI76_RS11130 0.50 +0.2 2,347,442 - BWI76_RS11130 0.61 -3.0 2,347,518 + BWI76_RS11130 0.67 +0.7 2,347,526 - BWI76_RS11130 0.67 -1.7 2,347,736 - BWI76_RS11130 0.82 -0.1 2,348,199 - -2.6 2,348,210 - +0.6 2,348,255 + -2.9 2,348,408 + BWI76_RS11135 0.14 +1.0 2,348,416 - BWI76_RS11135 0.15 +2.0 2,348,416 - BWI76_RS11135 0.15 -2.3 2,348,467 - BWI76_RS11135 0.17 +2.8 2,348,748 + BWI76_RS11135 0.30 +0.5 2,348,765 - BWI76_RS11135 0.31 -2.1 2,349,072 + BWI76_RS11135 0.45 +0.5 2,349,085 - BWI76_RS11135 0.45 +0.8 2,349,811 + BWI76_RS11135 0.78 +0.1 2,349,968 + BWI76_RS11135 0.86 +2.3 2,351,192 - BWI76_RS11150 0.52 +0.2
Or see this region's nucleotide sequence