Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS09740

Experiment: D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS09735 and BWI76_RS09740 are separated by 51 nucleotidesBWI76_RS09740 and BWI76_RS09745 are separated by 382 nucleotides BWI76_RS09735: BWI76_RS09735 - formate acetyltransferase, at 2,039,827 to 2,042,109 _RS09735 BWI76_RS09740: BWI76_RS09740 - formate transporter FocA, at 2,042,161 to 2,043,018 _RS09740 BWI76_RS09745: BWI76_RS09745 - 30S ribosomal protein S12 methylthiotransferase accessory protein YcaO, at 2,043,401 to 2,045,161 _RS09745 Position (kb) 2042 2043 2044Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 2041.439 kb on - strand, within BWI76_RS09735at 2041.554 kb on - strand, within BWI76_RS09735at 2042.081 kb on + strandat 2042.484 kb on + strand, within BWI76_RS09740at 2042.584 kb on + strand, within BWI76_RS09740at 2042.672 kb on - strand, within BWI76_RS09740at 2042.754 kb on + strand, within BWI76_RS09740at 2042.864 kb on - strand, within BWI76_RS09740at 2042.884 kb on + strand, within BWI76_RS09740at 2042.884 kb on + strand, within BWI76_RS09740at 2042.884 kb on + strand, within BWI76_RS09740at 2042.884 kb on + strand, within BWI76_RS09740at 2042.892 kb on - strand, within BWI76_RS09740at 2042.892 kb on - strand, within BWI76_RS09740at 2042.892 kb on - strand, within BWI76_RS09740at 2042.892 kb on - strand, within BWI76_RS09740at 2042.892 kb on - strand, within BWI76_RS09740at 2042.900 kb on - strand, within BWI76_RS09740at 2042.902 kb on - strand, within BWI76_RS09740at 2042.902 kb on - strand, within BWI76_RS09740at 2042.910 kb on + strand, within BWI76_RS09740at 2042.976 kb on + strandat 2042.978 kb on + strandat 2043.004 kb on + strandat 2043.018 kb on + strandat 2043.026 kb on - strandat 2043.028 kb on + strandat 2043.030 kb on - strandat 2043.030 kb on - strandat 2043.063 kb on + strandat 2043.063 kb on + strandat 2043.071 kb on - strandat 2043.071 kb on - strandat 2043.071 kb on - strandat 2043.088 kb on + strandat 2043.088 kb on + strandat 2043.088 kb on + strandat 2043.088 kb on + strandat 2043.088 kb on + strandat 2043.088 kb on + strandat 2043.088 kb on + strandat 2043.088 kb on + strandat 2043.088 kb on + strandat 2043.088 kb on + strandat 2043.096 kb on - strandat 2043.096 kb on - strandat 2043.096 kb on - strandat 2043.096 kb on - strandat 2043.096 kb on - strandat 2043.097 kb on - strandat 2043.097 kb on - strandat 2043.121 kb on - strandat 2043.345 kb on + strandat 2043.447 kb on + strandat 2043.455 kb on - strandat 2043.530 kb on - strandat 2043.613 kb on - strand, within BWI76_RS09745at 2043.635 kb on + strand, within BWI76_RS09745at 2043.689 kb on + strand, within BWI76_RS09745at 2043.711 kb on - strand, within BWI76_RS09745at 2043.716 kb on - strand, within BWI76_RS09745at 2043.778 kb on + strand, within BWI76_RS09745at 2043.786 kb on - strand, within BWI76_RS09745

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days
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2,041,439 - BWI76_RS09735 0.71 +0.1
2,041,554 - BWI76_RS09735 0.76 -0.9
2,042,081 + -4.4
2,042,484 + BWI76_RS09740 0.38 -2.7
2,042,584 + BWI76_RS09740 0.49 -0.7
2,042,672 - BWI76_RS09740 0.60 -1.0
2,042,754 + BWI76_RS09740 0.69 -2.0
2,042,864 - BWI76_RS09740 0.82 -0.4
2,042,884 + BWI76_RS09740 0.84 -4.2
2,042,884 + BWI76_RS09740 0.84 -1.1
2,042,884 + BWI76_RS09740 0.84 -1.7
2,042,884 + BWI76_RS09740 0.84 -1.1
2,042,892 - BWI76_RS09740 0.85 -5.2
2,042,892 - BWI76_RS09740 0.85 -3.8
2,042,892 - BWI76_RS09740 0.85 +0.1
2,042,892 - BWI76_RS09740 0.85 -0.8
2,042,892 - BWI76_RS09740 0.85 +0.4
2,042,900 - BWI76_RS09740 0.86 -0.4
2,042,902 - BWI76_RS09740 0.86 -2.3
2,042,902 - BWI76_RS09740 0.86 -0.6
2,042,910 + BWI76_RS09740 0.87 -2.2
2,042,976 + -3.6
2,042,978 + -2.5
2,043,004 + -0.9
2,043,018 + -4.6
2,043,026 - -3.2
2,043,028 + -3.0
2,043,030 - -0.5
2,043,030 - -1.1
2,043,063 + +0.7
2,043,063 + +1.0
2,043,071 - +0.2
2,043,071 - -1.3
2,043,071 - +0.4
2,043,088 + +1.0
2,043,088 + -0.4
2,043,088 + +0.6
2,043,088 + +1.3
2,043,088 + +1.3
2,043,088 + +0.5
2,043,088 + -1.9
2,043,088 + +0.1
2,043,088 + -0.7
2,043,088 + +0.1
2,043,096 - +0.5
2,043,096 - -0.3
2,043,096 - +2.0
2,043,096 - -0.0
2,043,096 - +1.6
2,043,097 - +0.9
2,043,097 - +1.2
2,043,121 - -0.2
2,043,345 + +0.1
2,043,447 + +0.5
2,043,455 - +0.5
2,043,530 - +0.1
2,043,613 - BWI76_RS09745 0.12 +0.8
2,043,635 + BWI76_RS09745 0.13 +0.9
2,043,689 + BWI76_RS09745 0.16 -1.9
2,043,711 - BWI76_RS09745 0.18 -0.0
2,043,716 - BWI76_RS09745 0.18 -0.6
2,043,778 + BWI76_RS09745 0.21 +1.5
2,043,786 - BWI76_RS09745 0.22 +0.7

Or see this region's nucleotide sequence