Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS04675

Experiment: D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS04670 and BWI76_RS04675 are separated by 2 nucleotidesBWI76_RS04675 and BWI76_RS04680 overlap by 1 nucleotides BWI76_RS04670: BWI76_RS04670 - 3-isopropylmalate dehydratase large subunit, at 973,685 to 975,085 _RS04670 BWI76_RS04675: BWI76_RS04675 - 3-isopropylmalate dehydrogenase, at 975,088 to 976,179 _RS04675 BWI76_RS04680: BWI76_RS04680 - 2-isopropylmalate synthase, at 976,179 to 977,750 _RS04680 Position (kb) 975 976 977Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 974.120 kb on + strand, within BWI76_RS04670at 974.272 kb on - strand, within BWI76_RS04670at 974.372 kb on + strand, within BWI76_RS04670at 974.395 kb on - strand, within BWI76_RS04670at 974.433 kb on + strand, within BWI76_RS04670at 974.439 kb on - strand, within BWI76_RS04670at 974.477 kb on + strand, within BWI76_RS04670at 974.480 kb on - strand, within BWI76_RS04670at 974.741 kb on - strandat 974.797 kb on + strand, within BWI76_RS04670at 974.894 kb on + strand, within BWI76_RS04670at 974.924 kb on - strand, within BWI76_RS04670at 974.924 kb on - strand, within BWI76_RS04670at 975.006 kb on + strandat 975.145 kb on - strandat 975.438 kb on + strand, within BWI76_RS04675at 975.473 kb on + strand, within BWI76_RS04675at 975.788 kb on - strand, within BWI76_RS04675at 975.833 kb on - strand, within BWI76_RS04675at 975.841 kb on - strand, within BWI76_RS04675at 975.899 kb on + strand, within BWI76_RS04675at 975.900 kb on - strand, within BWI76_RS04675at 976.189 kb on + strandat 976.321 kb on - strandat 976.321 kb on - strandat 976.368 kb on - strand, within BWI76_RS04680at 976.375 kb on + strand, within BWI76_RS04680at 976.383 kb on - strand, within BWI76_RS04680at 976.388 kb on - strand, within BWI76_RS04680at 976.423 kb on - strand, within BWI76_RS04680at 976.517 kb on + strand, within BWI76_RS04680at 976.628 kb on + strand, within BWI76_RS04680at 976.696 kb on - strand, within BWI76_RS04680at 976.775 kb on + strand, within BWI76_RS04680at 976.807 kb on - strand, within BWI76_RS04680at 976.858 kb on + strand, within BWI76_RS04680at 976.895 kb on - strand, within BWI76_RS04680at 976.918 kb on + strand, within BWI76_RS04680at 977.079 kb on - strand, within BWI76_RS04680at 977.084 kb on - strand, within BWI76_RS04680at 977.091 kb on + strand, within BWI76_RS04680at 977.104 kb on - strand, within BWI76_RS04680at 977.135 kb on - strand, within BWI76_RS04680at 977.172 kb on + strand, within BWI76_RS04680

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days
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974,120 + BWI76_RS04670 0.31 -0.7
974,272 - BWI76_RS04670 0.42 -3.9
974,372 + BWI76_RS04670 0.49 -1.3
974,395 - BWI76_RS04670 0.51 -3.8
974,433 + BWI76_RS04670 0.53 -1.6
974,439 - BWI76_RS04670 0.54 -2.0
974,477 + BWI76_RS04670 0.57 +0.4
974,480 - BWI76_RS04670 0.57 -4.4
974,741 - +2.5
974,797 + BWI76_RS04670 0.79 -2.7
974,894 + BWI76_RS04670 0.86 -2.6
974,924 - BWI76_RS04670 0.88 -3.2
974,924 - BWI76_RS04670 0.88 -1.1
975,006 + -0.6
975,145 - -1.6
975,438 + BWI76_RS04675 0.32 -4.3
975,473 + BWI76_RS04675 0.35 -4.1
975,788 - BWI76_RS04675 0.64 -2.6
975,833 - BWI76_RS04675 0.68 +0.1
975,841 - BWI76_RS04675 0.69 -3.3
975,899 + BWI76_RS04675 0.74 -2.3
975,900 - BWI76_RS04675 0.74 -0.9
976,189 + -2.8
976,321 - -0.4
976,321 - -1.2
976,368 - BWI76_RS04680 0.12 -0.5
976,375 + BWI76_RS04680 0.12 -3.6
976,383 - BWI76_RS04680 0.13 +0.5
976,388 - BWI76_RS04680 0.13 +0.9
976,423 - BWI76_RS04680 0.16 -0.1
976,517 + BWI76_RS04680 0.22 -1.8
976,628 + BWI76_RS04680 0.29 -4.4
976,696 - BWI76_RS04680 0.33 -0.4
976,775 + BWI76_RS04680 0.38 -0.0
976,807 - BWI76_RS04680 0.40 -1.3
976,858 + BWI76_RS04680 0.43 -3.1
976,895 - BWI76_RS04680 0.46 -0.7
976,918 + BWI76_RS04680 0.47 -0.6
977,079 - BWI76_RS04680 0.57 -0.3
977,084 - BWI76_RS04680 0.58 -4.8
977,091 + BWI76_RS04680 0.58 -1.5
977,104 - BWI76_RS04680 0.59 -1.4
977,135 - BWI76_RS04680 0.61 -1.3
977,172 + BWI76_RS04680 0.63 -2.3

Or see this region's nucleotide sequence