Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS03595

Experiment: D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS03590 and BWI76_RS03595 are separated by 53 nucleotidesBWI76_RS03595 and BWI76_RS03600 are separated by 15 nucleotidesBWI76_RS03600 and BWI76_RS03605 are separated by 17 nucleotides BWI76_RS03590: BWI76_RS03590 - acetaldehyde dehydrogenase (acetylating), at 744,308 to 745,909 _RS03590 BWI76_RS03595: BWI76_RS03595 - ethanolamine utilization protein EutM, at 745,963 to 746,241 _RS03595 BWI76_RS03600: BWI76_RS03600 - BMC domain-containing protein, at 746,257 to 746,541 _RS03600 BWI76_RS03605: BWI76_RS03605 - BMC domain-containing protein, at 746,559 to 746,837 _RS03605 Position (kb) 745 746 747Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 744.984 kb on - strand, within BWI76_RS03590at 745.253 kb on - strand, within BWI76_RS03590at 745.306 kb on - strand, within BWI76_RS03590at 745.306 kb on - strand, within BWI76_RS03590at 745.354 kb on + strand, within BWI76_RS03590at 745.362 kb on - strand, within BWI76_RS03590at 745.450 kb on + strand, within BWI76_RS03590at 745.869 kb on - strandat 745.883 kb on + strandat 746.034 kb on - strand, within BWI76_RS03595at 746.173 kb on - strand, within BWI76_RS03595at 746.505 kb on - strand, within BWI76_RS03600at 746.523 kb on + strandat 746.557 kb on - strandat 746.557 kb on - strandat 746.695 kb on + strand, within BWI76_RS03605at 746.725 kb on - strand, within BWI76_RS03605at 746.769 kb on - strand, within BWI76_RS03605at 746.819 kb on + strandat 746.819 kb on + strandat 746.827 kb on - strandat 746.827 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days
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744,984 - BWI76_RS03590 0.42 +2.8
745,253 - BWI76_RS03590 0.59 -0.2
745,306 - BWI76_RS03590 0.62 -0.4
745,306 - BWI76_RS03590 0.62 +0.6
745,354 + BWI76_RS03590 0.65 -3.3
745,362 - BWI76_RS03590 0.66 +1.9
745,450 + BWI76_RS03590 0.71 +0.6
745,869 - +0.0
745,883 + +0.6
746,034 - BWI76_RS03595 0.25 +1.9
746,173 - BWI76_RS03595 0.75 +2.2
746,505 - BWI76_RS03600 0.87 +2.8
746,523 + +0.7
746,557 - +1.5
746,557 - +0.8
746,695 + BWI76_RS03605 0.49 -1.4
746,725 - BWI76_RS03605 0.59 +0.5
746,769 - BWI76_RS03605 0.75 -2.6
746,819 + -3.1
746,819 + +0.8
746,827 - +0.4
746,827 - +0.4

Or see this region's nucleotide sequence