Experiment: D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03535 and BWI76_RS03540 are separated by 123 nucleotides BWI76_RS03540 and BWI76_RS03545 are separated by 241 nucleotides BWI76_RS03545 and BWI76_RS03550 are separated by 17 nucleotides
BWI76_RS03535: BWI76_RS03535 - Tryptophanase (EC 4.1.99.1) <small>(from data)</small>, at 734,065 to 735,453
_RS03535
BWI76_RS03540: BWI76_RS03540 - tryptophanase leader peptide, at 735,577 to 735,672
_RS03540
BWI76_RS03545: BWI76_RS03545 - QacE family quaternary ammonium compound efflux SMR transporter, at 735,914 to 736,243
_RS03545
BWI76_RS03550: BWI76_RS03550 - QacE family quaternary ammonium compound efflux SMR transporter, at 736,261 to 736,680
_RS03550
Position (kb) 735
736 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 734.609 kb on + strand, within BWI76_RS03535 at 734.639 kb on - strand, within BWI76_RS03535 at 734.644 kb on - strand, within BWI76_RS03535 at 734.686 kb on + strand, within BWI76_RS03535 at 734.691 kb on + strand, within BWI76_RS03535 at 734.691 kb on + strand, within BWI76_RS03535 at 734.691 kb on + strand, within BWI76_RS03535 at 734.691 kb on + strand, within BWI76_RS03535 at 734.691 kb on + strand, within BWI76_RS03535 at 734.691 kb on + strand, within BWI76_RS03535 at 734.694 kb on - strand, within BWI76_RS03535 at 734.694 kb on - strand, within BWI76_RS03535 at 734.699 kb on - strand, within BWI76_RS03535 at 734.699 kb on - strand, within BWI76_RS03535 at 734.699 kb on - strand, within BWI76_RS03535 at 734.706 kb on + strand, within BWI76_RS03535 at 734.744 kb on + strand, within BWI76_RS03535 at 734.752 kb on - strand, within BWI76_RS03535 at 734.752 kb on - strand, within BWI76_RS03535 at 734.763 kb on + strand, within BWI76_RS03535 at 734.836 kb on + strand, within BWI76_RS03535 at 734.842 kb on - strand, within BWI76_RS03535 at 734.844 kb on - strand, within BWI76_RS03535 at 734.885 kb on + strand, within BWI76_RS03535 at 734.887 kb on + strand, within BWI76_RS03535 at 734.893 kb on + strand, within BWI76_RS03535 at 734.940 kb on - strand, within BWI76_RS03535 at 734.942 kb on - strand, within BWI76_RS03535 at 734.989 kb on + strand, within BWI76_RS03535 at 734.989 kb on + strand, within BWI76_RS03535 at 735.009 kb on - strand, within BWI76_RS03535 at 735.047 kb on + strand, within BWI76_RS03535 at 735.144 kb on + strand, within BWI76_RS03535 at 735.157 kb on - strand, within BWI76_RS03535 at 735.159 kb on + strand, within BWI76_RS03535 at 735.174 kb on - strand, within BWI76_RS03535 at 735.176 kb on + strand, within BWI76_RS03535 at 735.176 kb on + strand, within BWI76_RS03535 at 735.176 kb on + strand, within BWI76_RS03535 at 735.183 kb on - strand, within BWI76_RS03535 at 735.232 kb on - strand, within BWI76_RS03535 at 735.238 kb on + strand, within BWI76_RS03535 at 735.266 kb on - strand, within BWI76_RS03535 at 735.284 kb on - strand, within BWI76_RS03535 at 735.294 kb on - strand, within BWI76_RS03535 at 735.299 kb on - strand, within BWI76_RS03535 at 735.299 kb on - strand, within BWI76_RS03535 at 735.315 kb on + strand at 735.320 kb on + strand at 735.320 kb on + strand at 735.327 kb on - strand at 735.362 kb on + strand at 735.362 kb on + strand at 735.366 kb on + strand at 735.374 kb on + strand at 735.375 kb on - strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.395 kb on - strand at 735.395 kb on - strand at 735.395 kb on - strand at 735.395 kb on - strand at 735.395 kb on - strand at 735.395 kb on - strand at 735.395 kb on - strand at 735.395 kb on - strand at 735.395 kb on - strand at 735.409 kb on + strand at 735.433 kb on + strand at 735.435 kb on + strand at 735.435 kb on + strand at 735.435 kb on + strand at 735.435 kb on + strand at 735.435 kb on + strand at 735.435 kb on + strand at 735.443 kb on - strand at 735.443 kb on - strand at 735.443 kb on - strand at 735.443 kb on - strand at 735.443 kb on - strand at 735.443 kb on - strand at 735.468 kb on + strand at 735.481 kb on + strand at 735.596 kb on - strand, within BWI76_RS03540 at 735.611 kb on + strand, within BWI76_RS03540 at 735.612 kb on + strand, within BWI76_RS03540 at 735.614 kb on - strand, within BWI76_RS03540 at 735.634 kb on + strand, within BWI76_RS03540 at 735.634 kb on + strand, within BWI76_RS03540 at 735.634 kb on + strand, within BWI76_RS03540 at 735.634 kb on + strand, within BWI76_RS03540 at 735.639 kb on + strand, within BWI76_RS03540 at 735.642 kb on - strand, within BWI76_RS03540 at 735.674 kb on + strand at 735.682 kb on - strand at 735.688 kb on + strand at 735.697 kb on - strand at 735.770 kb on + strand at 735.803 kb on + strand at 735.806 kb on + strand at 735.827 kb on - strand at 735.880 kb on + strand at 735.912 kb on + strand at 735.912 kb on + strand at 735.913 kb on + strand at 735.914 kb on - strand at 735.920 kb on - strand at 735.964 kb on - strand, within BWI76_RS03545 at 735.964 kb on - strand, within BWI76_RS03545 at 735.974 kb on - strand, within BWI76_RS03545 at 736.036 kb on + strand, within BWI76_RS03545 at 736.038 kb on + strand, within BWI76_RS03545 at 736.075 kb on + strand, within BWI76_RS03545 at 736.075 kb on + strand, within BWI76_RS03545 at 736.094 kb on + strand, within BWI76_RS03545 at 736.102 kb on - strand, within BWI76_RS03545 at 736.104 kb on + strand, within BWI76_RS03545 at 736.112 kb on - strand, within BWI76_RS03545 at 736.149 kb on + strand, within BWI76_RS03545 at 736.457 kb on - strand, within BWI76_RS03550 at 736.478 kb on + strand, within BWI76_RS03550 at 736.478 kb on + strand, within BWI76_RS03550 at 736.478 kb on + strand, within BWI76_RS03550 at 736.491 kb on - strand, within BWI76_RS03550 at 736.491 kb on - strand, within BWI76_RS03550 at 736.509 kb on - strand, within BWI76_RS03550 at 736.548 kb on - strand, within BWI76_RS03550 at 736.608 kb on + strand, within BWI76_RS03550
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days remove 734,609 + BWI76_RS03535 0.39 +0.5 734,639 - BWI76_RS03535 0.41 -0.2 734,644 - BWI76_RS03535 0.42 +0.3 734,686 + BWI76_RS03535 0.45 -0.3 734,691 + BWI76_RS03535 0.45 -0.6 734,691 + BWI76_RS03535 0.45 +0.7 734,691 + BWI76_RS03535 0.45 +2.0 734,691 + BWI76_RS03535 0.45 +0.6 734,691 + BWI76_RS03535 0.45 +0.4 734,691 + BWI76_RS03535 0.45 +0.1 734,694 - BWI76_RS03535 0.45 -0.2 734,694 - BWI76_RS03535 0.45 +0.1 734,699 - BWI76_RS03535 0.46 +0.6 734,699 - BWI76_RS03535 0.46 -0.3 734,699 - BWI76_RS03535 0.46 -0.5 734,706 + BWI76_RS03535 0.46 -0.6 734,744 + BWI76_RS03535 0.49 +0.8 734,752 - BWI76_RS03535 0.49 +0.3 734,752 - BWI76_RS03535 0.49 -0.2 734,763 + BWI76_RS03535 0.50 +0.5 734,836 + BWI76_RS03535 0.56 +1.0 734,842 - BWI76_RS03535 0.56 +1.7 734,844 - BWI76_RS03535 0.56 -0.5 734,885 + BWI76_RS03535 0.59 +0.1 734,887 + BWI76_RS03535 0.59 +1.8 734,893 + BWI76_RS03535 0.60 -2.0 734,940 - BWI76_RS03535 0.63 -2.2 734,942 - BWI76_RS03535 0.63 -0.0 734,989 + BWI76_RS03535 0.67 +0.7 734,989 + BWI76_RS03535 0.67 +0.9 735,009 - BWI76_RS03535 0.68 +0.6 735,047 + BWI76_RS03535 0.71 -0.8 735,144 + BWI76_RS03535 0.78 -0.1 735,157 - BWI76_RS03535 0.79 +0.1 735,159 + BWI76_RS03535 0.79 +0.0 735,174 - BWI76_RS03535 0.80 -5.2 735,176 + BWI76_RS03535 0.80 +1.3 735,176 + BWI76_RS03535 0.80 +0.6 735,176 + BWI76_RS03535 0.80 +0.2 735,183 - BWI76_RS03535 0.80 +1.1 735,232 - BWI76_RS03535 0.84 +0.4 735,238 + BWI76_RS03535 0.84 -0.0 735,266 - BWI76_RS03535 0.86 -0.3 735,284 - BWI76_RS03535 0.88 +0.6 735,294 - BWI76_RS03535 0.88 -2.8 735,299 - BWI76_RS03535 0.89 -4.4 735,299 - BWI76_RS03535 0.89 +0.0 735,315 + +1.5 735,320 + -3.7 735,320 + +0.8 735,327 - -0.0 735,362 + +1.9 735,362 + -1.6 735,366 + -0.6 735,374 + +0.6 735,375 - -2.9 735,387 + +1.6 735,387 + +0.3 735,387 + +0.8 735,387 + -1.0 735,387 + +1.2 735,387 + -3.4 735,387 + -0.7 735,387 + -1.0 735,387 + +1.4 735,387 + -3.3 735,387 + +1.6 735,387 + -3.4 735,387 + -2.2 735,387 + +1.8 735,387 + -3.3 735,387 + -2.6 735,387 + +0.6 735,387 + +0.4 735,387 + -1.9 735,395 - +1.1 735,395 - -0.0 735,395 - +0.5 735,395 - -2.1 735,395 - +0.9 735,395 - +0.3 735,395 - -0.1 735,395 - -0.1 735,395 - +1.5 735,409 + -0.9 735,433 + -0.0 735,435 + +2.2 735,435 + +1.6 735,435 + +0.6 735,435 + -0.5 735,435 + +0.1 735,435 + +0.3 735,443 - -1.2 735,443 - -0.3 735,443 - -0.8 735,443 - -2.8 735,443 - +3.3 735,443 - -0.0 735,468 + -4.0 735,481 + +1.3 735,596 - BWI76_RS03540 0.20 +0.1 735,611 + BWI76_RS03540 0.35 -2.8 735,612 + BWI76_RS03540 0.36 -1.4 735,614 - BWI76_RS03540 0.39 -3.2 735,634 + BWI76_RS03540 0.59 -0.0 735,634 + BWI76_RS03540 0.59 -1.0 735,634 + BWI76_RS03540 0.59 -1.1 735,634 + BWI76_RS03540 0.59 +0.2 735,639 + BWI76_RS03540 0.65 -3.6 735,642 - BWI76_RS03540 0.68 -0.5 735,674 + +0.0 735,682 - -0.5 735,688 + +0.4 735,697 - -1.5 735,770 + +0.6 735,803 + +1.5 735,806 + -0.1 735,827 - -4.1 735,880 + +1.0 735,912 + -0.0 735,912 + -0.2 735,913 + -0.8 735,914 - +1.8 735,920 - +1.0 735,964 - BWI76_RS03545 0.15 +1.2 735,964 - BWI76_RS03545 0.15 -3.4 735,974 - BWI76_RS03545 0.18 -2.6 736,036 + BWI76_RS03545 0.37 +0.9 736,038 + BWI76_RS03545 0.38 +0.8 736,075 + BWI76_RS03545 0.49 +0.2 736,075 + BWI76_RS03545 0.49 +0.3 736,094 + BWI76_RS03545 0.55 -1.7 736,102 - BWI76_RS03545 0.57 +1.8 736,104 + BWI76_RS03545 0.58 -0.1 736,112 - BWI76_RS03545 0.60 +0.4 736,149 + BWI76_RS03545 0.71 +1.5 736,457 - BWI76_RS03550 0.47 +1.5 736,478 + BWI76_RS03550 0.52 -1.7 736,478 + BWI76_RS03550 0.52 +0.3 736,478 + BWI76_RS03550 0.52 +0.1 736,491 - BWI76_RS03550 0.55 -3.2 736,491 - BWI76_RS03550 0.55 +0.2 736,509 - BWI76_RS03550 0.59 -3.3 736,548 - BWI76_RS03550 0.68 -0.2 736,608 + BWI76_RS03550 0.83 -0.5
Or see this region's nucleotide sequence