Experiment: D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02925 and BWI76_RS02930 are separated by 17 nucleotides BWI76_RS02930 and BWI76_RS02935 are separated by 257 nucleotides BWI76_RS02935 and BWI76_RS02940 are separated by 369 nucleotides
BWI76_RS02925: BWI76_RS02925 - type IV secretion protein Rhs, at 596,405 to 598,123
_RS02925
BWI76_RS02930: BWI76_RS02930 - anaerobic ribonucleoside-triphosphate reductase activating protein, at 598,141 to 598,605
_RS02930
BWI76_RS02935: BWI76_RS02935 - anaerobic ribonucleoside triphosphate reductase, at 598,863 to 601,001
_RS02935
BWI76_RS02940: BWI76_RS02940 - RpiR family transcriptional regulator, at 601,371 to 602,114
_RS02940
Position (kb) 598
599
600
601
602 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 597.921 kb on - strand, within BWI76_RS02925 at 598.153 kb on - strand at 598.281 kb on + strand, within BWI76_RS02930 at 598.521 kb on + strand, within BWI76_RS02930 at 598.552 kb on + strand, within BWI76_RS02930 at 598.559 kb on + strand at 598.591 kb on - strand at 598.853 kb on - strand at 598.924 kb on + strand at 599.019 kb on + strand at 599.027 kb on - strand at 599.118 kb on + strand, within BWI76_RS02935 at 599.143 kb on + strand, within BWI76_RS02935 at 599.144 kb on + strand, within BWI76_RS02935 at 599.152 kb on - strand, within BWI76_RS02935 at 599.209 kb on + strand, within BWI76_RS02935 at 599.243 kb on + strand at 599.251 kb on - strand, within BWI76_RS02935 at 599.261 kb on - strand, within BWI76_RS02935 at 599.376 kb on + strand, within BWI76_RS02935 at 599.381 kb on + strand, within BWI76_RS02935 at 599.381 kb on + strand, within BWI76_RS02935 at 599.388 kb on + strand, within BWI76_RS02935 at 599.389 kb on - strand, within BWI76_RS02935 at 599.465 kb on + strand, within BWI76_RS02935 at 599.610 kb on - strand, within BWI76_RS02935 at 599.610 kb on - strand, within BWI76_RS02935 at 599.788 kb on - strand, within BWI76_RS02935 at 599.802 kb on + strand, within BWI76_RS02935 at 599.815 kb on - strand, within BWI76_RS02935 at 599.959 kb on - strand, within BWI76_RS02935 at 600.233 kb on - strand, within BWI76_RS02935 at 600.331 kb on + strand, within BWI76_RS02935 at 600.406 kb on - strand, within BWI76_RS02935 at 600.580 kb on + strand, within BWI76_RS02935 at 600.620 kb on + strand, within BWI76_RS02935 at 600.620 kb on + strand, within BWI76_RS02935 at 600.670 kb on + strand, within BWI76_RS02935 at 600.678 kb on - strand, within BWI76_RS02935 at 600.755 kb on - strand, within BWI76_RS02935 at 600.997 kb on + strand at 600.997 kb on - strand at 601.009 kb on + strand at 601.049 kb on - strand at 601.115 kb on + strand at 601.156 kb on - strand at 601.285 kb on - strand at 601.395 kb on - strand at 601.442 kb on - strand at 601.446 kb on - strand, within BWI76_RS02940 at 601.535 kb on + strand, within BWI76_RS02940 at 601.699 kb on + strand, within BWI76_RS02940 at 601.887 kb on - strand, within BWI76_RS02940
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days remove 597,921 - BWI76_RS02925 0.88 -1.1 598,153 - -0.3 598,281 + BWI76_RS02930 0.30 -2.2 598,521 + BWI76_RS02930 0.82 -1.1 598,552 + BWI76_RS02930 0.88 -1.9 598,559 + -2.2 598,591 - -2.7 598,853 - -0.3 598,924 + -3.3 599,019 + -0.9 599,027 - -2.2 599,118 + BWI76_RS02935 0.12 -1.0 599,143 + BWI76_RS02935 0.13 -4.3 599,144 + BWI76_RS02935 0.13 -3.6 599,152 - BWI76_RS02935 0.14 -1.2 599,209 + BWI76_RS02935 0.16 -4.5 599,243 + +0.3 599,251 - BWI76_RS02935 0.18 -0.5 599,261 - BWI76_RS02935 0.19 -2.9 599,376 + BWI76_RS02935 0.24 -2.3 599,381 + BWI76_RS02935 0.24 +0.2 599,381 + BWI76_RS02935 0.24 -1.9 599,388 + BWI76_RS02935 0.25 -3.0 599,389 - BWI76_RS02935 0.25 -1.4 599,465 + BWI76_RS02935 0.28 -3.3 599,610 - BWI76_RS02935 0.35 -2.8 599,610 - BWI76_RS02935 0.35 -0.7 599,788 - BWI76_RS02935 0.43 -4.1 599,802 + BWI76_RS02935 0.44 -0.5 599,815 - BWI76_RS02935 0.45 -1.9 599,959 - BWI76_RS02935 0.51 +1.1 600,233 - BWI76_RS02935 0.64 +0.1 600,331 + BWI76_RS02935 0.69 -5.0 600,406 - BWI76_RS02935 0.72 -0.7 600,580 + BWI76_RS02935 0.80 -4.4 600,620 + BWI76_RS02935 0.82 -5.5 600,620 + BWI76_RS02935 0.82 -1.1 600,670 + BWI76_RS02935 0.84 -0.6 600,678 - BWI76_RS02935 0.85 -3.3 600,755 - BWI76_RS02935 0.88 -3.2 600,997 + -1.7 600,997 - +0.3 601,009 + -1.2 601,049 - -0.3 601,115 + -0.7 601,156 - -0.3 601,285 - -0.8 601,395 - +0.1 601,442 - -0.0 601,446 - BWI76_RS02940 0.10 -0.9 601,535 + BWI76_RS02940 0.22 +0.0 601,699 + BWI76_RS02940 0.44 +0.2 601,887 - BWI76_RS02940 0.69 +0.6
Or see this region's nucleotide sequence