Experiment: PEG (Day 1) - Cage4;Mouse1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3868 and BT3869 are separated by 146 nucleotides BT3869 and BT3870 are separated by 5 nucleotides BT3870 and tRNA-Val are separated by 235 nucleotides tRNA-Val and tRNA-Val are separated by 29 nucleotides
BT3868: BT3868 - beta-N-hexosaminidase, glycosyl hyrolase family 20 (NCBI ptt file), at 5,039,698 to 5,041,683
BT3868
BT3869: BT3869 - conserved hypothetical protein (NCBI ptt file), at 5,041,830 to 5,042,603
BT3869
BT3870: BT3870 - putative maltose O-acetyltransferase (NCBI ptt file), at 5,042,609 to 5,043,160
BT3870
BT_t62: tRNA-Val - tRNA-Val (RefSeq), at 5,043,396 to 5,043,473
tRNA-Val
BT_t63: tRNA-Val - tRNA-Val (RefSeq), at 5,043,503 to 5,043,580
tRNA-Val
Position (kb)
5041
5042
5043 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5041.013 kb on + strand, within BT3868 at 5041.043 kb on + strand, within BT3868 at 5041.043 kb on + strand, within BT3868 at 5041.070 kb on + strand, within BT3868 at 5041.070 kb on + strand, within BT3868 at 5041.125 kb on - strand, within BT3868 at 5041.125 kb on - strand, within BT3868 at 5041.154 kb on + strand, within BT3868 at 5041.187 kb on - strand, within BT3868 at 5041.187 kb on - strand, within BT3868 at 5041.187 kb on - strand, within BT3868 at 5041.327 kb on + strand, within BT3868 at 5041.457 kb on + strand, within BT3868 at 5041.490 kb on - strand at 5041.546 kb on - strand at 5041.546 kb on - strand at 5041.620 kb on + strand at 5041.667 kb on - strand at 5041.733 kb on - strand at 5041.838 kb on - strand at 5042.070 kb on + strand, within BT3869 at 5042.083 kb on + strand, within BT3869 at 5042.291 kb on - strand, within BT3869 at 5042.438 kb on - strand, within BT3869 at 5042.445 kb on + strand, within BT3869 at 5042.445 kb on + strand, within BT3869 at 5042.582 kb on + strand at 5042.582 kb on + strand at 5042.583 kb on - strand at 5042.602 kb on - strand at 5042.620 kb on - strand at 5042.651 kb on - strand at 5042.651 kb on - strand at 5042.726 kb on + strand, within BT3870 at 5042.726 kb on + strand, within BT3870 at 5042.726 kb on + strand, within BT3870 at 5042.863 kb on + strand, within BT3870 at 5042.863 kb on + strand, within BT3870 at 5042.882 kb on + strand, within BT3870 at 5042.882 kb on + strand, within BT3870 at 5042.882 kb on + strand, within BT3870 at 5042.883 kb on - strand, within BT3870 at 5042.883 kb on - strand, within BT3870 at 5042.884 kb on + strand, within BT3870 at 5042.903 kb on - strand, within BT3870 at 5042.930 kb on + strand, within BT3870 at 5042.930 kb on + strand, within BT3870 at 5042.930 kb on + strand, within BT3870 at 5042.931 kb on - strand, within BT3870 at 5042.981 kb on + strand, within BT3870 at 5043.583 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PEG (Day 1) - Cage4;Mouse1 remove 5,041,013 + BT3868 0.66 -0.4 5,041,043 + BT3868 0.68 +1.1 5,041,043 + BT3868 0.68 -0.9 5,041,070 + BT3868 0.69 -0.5 5,041,070 + BT3868 0.69 -2.0 5,041,125 - BT3868 0.72 +0.2 5,041,125 - BT3868 0.72 +0.9 5,041,154 + BT3868 0.73 +0.1 5,041,187 - BT3868 0.75 -2.5 5,041,187 - BT3868 0.75 +0.5 5,041,187 - BT3868 0.75 -0.9 5,041,327 + BT3868 0.82 -1.0 5,041,457 + BT3868 0.89 +0.5 5,041,490 - -1.1 5,041,546 - +2.5 5,041,546 - -0.4 5,041,620 + +0.6 5,041,667 - +0.5 5,041,733 - -0.1 5,041,838 - -1.8 5,042,070 + BT3869 0.31 -0.6 5,042,083 + BT3869 0.33 -1.7 5,042,291 - BT3869 0.60 -0.8 5,042,438 - BT3869 0.79 +0.8 5,042,445 + BT3869 0.79 -2.8 5,042,445 + BT3869 0.79 +0.5 5,042,582 + -0.8 5,042,582 + +0.2 5,042,583 - -2.5 5,042,602 - -0.9 5,042,620 - -1.7 5,042,651 - -1.1 5,042,651 - +1.5 5,042,726 + BT3870 0.21 -1.1 5,042,726 + BT3870 0.21 +0.1 5,042,726 + BT3870 0.21 -0.5 5,042,863 + BT3870 0.46 -0.5 5,042,863 + BT3870 0.46 +0.9 5,042,882 + BT3870 0.49 +0.6 5,042,882 + BT3870 0.49 -1.9 5,042,882 + BT3870 0.49 -1.1 5,042,883 - BT3870 0.50 +1.5 5,042,883 - BT3870 0.50 -2.2 5,042,884 + BT3870 0.50 -3.1 5,042,903 - BT3870 0.53 +0.8 5,042,930 + BT3870 0.58 -0.1 5,042,930 + BT3870 0.58 -1.1 5,042,930 + BT3870 0.58 -1.2 5,042,931 - BT3870 0.58 +0.1 5,042,981 + BT3870 0.67 -2.0 5,043,583 + -0.4
Or see this region's nucleotide sequence