Experiment: R2A control for volatile experiments
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa2321 and SMa2323 are separated by 324 nucleotides SMa2323 and SMa2325 are separated by 223 nucleotides SMa2325 and SMa2327 overlap by 4 nucleotides
SMa2321: SMa2321 - UvrD2 DNA helicase, at 1,293,347 to 1,295,416
SMa2321
SMa2323: SMa2323 - hypothetical protein, at 1,295,741 to 1,296,166
SMa2323
SMa2325: SMa2325 - transcriptional regulator, at 1,296,390 to 1,297,082
SMa2325
SMa2327: SMa2327 - sensor protein, at 1,297,079 to 1,299,769
SMa2327
Position (kb)
1295
1296
1297 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1294.833 kb on + strand, within SMa2321 at 1294.833 kb on + strand, within SMa2321 at 1294.833 kb on + strand, within SMa2321 at 1294.834 kb on - strand, within SMa2321 at 1294.834 kb on - strand, within SMa2321 at 1294.834 kb on - strand, within SMa2321 at 1294.875 kb on + strand, within SMa2321 at 1295.016 kb on + strand, within SMa2321 at 1295.030 kb on + strand, within SMa2321 at 1295.030 kb on + strand, within SMa2321 at 1295.030 kb on + strand, within SMa2321 at 1295.031 kb on - strand, within SMa2321 at 1295.031 kb on - strand, within SMa2321 at 1295.031 kb on - strand, within SMa2321 at 1295.031 kb on - strand, within SMa2321 at 1295.031 kb on - strand, within SMa2321 at 1295.035 kb on + strand, within SMa2321 at 1295.036 kb on - strand, within SMa2321 at 1295.171 kb on + strand, within SMa2321 at 1295.172 kb on - strand, within SMa2321 at 1295.172 kb on - strand, within SMa2321 at 1295.179 kb on + strand, within SMa2321 at 1295.180 kb on - strand, within SMa2321 at 1295.267 kb on + strand at 1295.360 kb on - strand at 1295.414 kb on + strand at 1295.488 kb on - strand at 1295.540 kb on - strand at 1295.540 kb on - strand at 1295.540 kb on - strand at 1295.540 kb on - strand at 1295.555 kb on + strand at 1295.556 kb on - strand at 1295.556 kb on - strand at 1295.625 kb on - strand at 1295.625 kb on - strand at 1295.634 kb on + strand at 1295.635 kb on - strand at 1295.645 kb on + strand at 1295.646 kb on - strand at 1295.682 kb on + strand at 1295.695 kb on + strand at 1295.695 kb on + strand at 1295.723 kb on + strand at 1295.724 kb on - strand at 1295.811 kb on + strand, within SMa2323 at 1295.909 kb on + strand, within SMa2323 at 1295.909 kb on + strand, within SMa2323 at 1295.937 kb on - strand, within SMa2323 at 1295.937 kb on - strand, within SMa2323 at 1295.996 kb on + strand, within SMa2323 at 1295.996 kb on + strand, within SMa2323 at 1295.996 kb on + strand, within SMa2323 at 1296.042 kb on - strand, within SMa2323 at 1296.122 kb on - strand, within SMa2323 at 1296.122 kb on - strand, within SMa2323 at 1296.122 kb on - strand, within SMa2323 at 1296.164 kb on + strand at 1296.165 kb on - strand at 1296.220 kb on + strand at 1296.220 kb on + strand at 1296.220 kb on + strand at 1296.221 kb on - strand at 1296.221 kb on - strand at 1296.506 kb on - strand, within SMa2325 at 1296.506 kb on - strand, within SMa2325 at 1296.506 kb on - strand, within SMa2325 at 1296.545 kb on + strand, within SMa2325 at 1296.545 kb on + strand, within SMa2325 at 1296.546 kb on - strand, within SMa2325 at 1296.607 kb on + strand, within SMa2325 at 1296.784 kb on + strand, within SMa2325 at 1296.785 kb on - strand, within SMa2325 at 1296.879 kb on - strand, within SMa2325 at 1296.990 kb on + strand, within SMa2325 at 1296.991 kb on - strand, within SMa2325
Per-strain Table
Position Strand Gene LocusTag Fraction R2A control for volatile experiments remove 1,294,833 + SMa2321 0.72 -0.5 1,294,833 + SMa2321 0.72 -1.3 1,294,833 + SMa2321 0.72 +0.3 1,294,834 - SMa2321 0.72 +0.3 1,294,834 - SMa2321 0.72 -1.1 1,294,834 - SMa2321 0.72 +0.8 1,294,875 + SMa2321 0.74 +0.8 1,295,016 + SMa2321 0.81 +1.1 1,295,030 + SMa2321 0.81 +0.7 1,295,030 + SMa2321 0.81 +1.4 1,295,030 + SMa2321 0.81 -1.5 1,295,031 - SMa2321 0.81 +1.5 1,295,031 - SMa2321 0.81 +0.1 1,295,031 - SMa2321 0.81 -0.1 1,295,031 - SMa2321 0.81 +0.2 1,295,031 - SMa2321 0.81 -0.1 1,295,035 + SMa2321 0.82 +0.0 1,295,036 - SMa2321 0.82 -1.1 1,295,171 + SMa2321 0.88 -0.7 1,295,172 - SMa2321 0.88 +0.6 1,295,172 - SMa2321 0.88 -0.3 1,295,179 + SMa2321 0.89 -2.5 1,295,180 - SMa2321 0.89 -2.8 1,295,267 + -1.6 1,295,360 - +0.2 1,295,414 + -2.6 1,295,488 - -0.3 1,295,540 - -0.6 1,295,540 - -0.7 1,295,540 - -0.8 1,295,540 - -1.8 1,295,555 + +1.1 1,295,556 - +0.1 1,295,556 - -0.2 1,295,625 - -0.1 1,295,625 - +0.5 1,295,634 + -0.1 1,295,635 - -0.4 1,295,645 + -1.8 1,295,646 - -0.9 1,295,682 + +0.5 1,295,695 + -0.1 1,295,695 + +0.1 1,295,723 + +1.1 1,295,724 - +0.1 1,295,811 + SMa2323 0.16 -1.2 1,295,909 + SMa2323 0.39 -2.0 1,295,909 + SMa2323 0.39 -1.4 1,295,937 - SMa2323 0.46 -2.2 1,295,937 - SMa2323 0.46 -0.4 1,295,996 + SMa2323 0.60 +0.9 1,295,996 + SMa2323 0.60 -0.6 1,295,996 + SMa2323 0.60 +0.2 1,296,042 - SMa2323 0.71 -0.4 1,296,122 - SMa2323 0.89 -1.6 1,296,122 - SMa2323 0.89 -0.3 1,296,122 - SMa2323 0.89 -2.9 1,296,164 + -1.5 1,296,165 - +1.5 1,296,220 + -2.8 1,296,220 + +0.0 1,296,220 + +0.0 1,296,221 - -0.2 1,296,221 - +1.4 1,296,506 - SMa2325 0.17 -0.7 1,296,506 - SMa2325 0.17 +0.1 1,296,506 - SMa2325 0.17 +1.8 1,296,545 + SMa2325 0.22 +0.6 1,296,545 + SMa2325 0.22 +0.5 1,296,546 - SMa2325 0.23 -1.5 1,296,607 + SMa2325 0.31 +0.6 1,296,784 + SMa2325 0.57 +0.6 1,296,785 - SMa2325 0.57 -0.1 1,296,879 - SMa2325 0.71 -0.0 1,296,990 + SMa2325 0.87 -0.5 1,296,991 - SMa2325 0.87 +0.4
Or see this region's nucleotide sequence