Experiment: R2A control for volatile experiments
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa5030 and SMa1337 are separated by 355 nucleotides SMa1337 and SMa1339 are separated by 142 nucleotides
SMa5030: SMa5030 - TetR family transcriptional regulator, at 730,316 to 730,981
SMa5030
SMa1337: SMa1337 - ABC transporter substrate-binding protein, at 731,337 to 732,644
SMa1337
SMa1339: SMa1339 - ABC transporter permease, at 732,787 to 733,692
SMa1339
Position (kb) 731
732
733 Strain fitness (log2 ratio) -2
-1
0
1 at 730.360 kb on + strand at 730.360 kb on + strand at 730.450 kb on + strand, within SMa5030 at 730.451 kb on - strand, within SMa5030 at 730.453 kb on - strand, within SMa5030 at 730.514 kb on + strand, within SMa5030 at 730.720 kb on + strand, within SMa5030 at 730.720 kb on + strand, within SMa5030 at 730.721 kb on - strand, within SMa5030 at 730.721 kb on - strand, within SMa5030 at 731.096 kb on - strand at 731.119 kb on + strand at 731.119 kb on + strand at 731.119 kb on + strand at 731.119 kb on + strand at 731.120 kb on - strand at 731.120 kb on - strand at 731.378 kb on + strand at 731.467 kb on + strand at 731.468 kb on - strand, within SMa1337 at 731.476 kb on - strand, within SMa1337 at 731.574 kb on + strand, within SMa1337 at 731.575 kb on - strand, within SMa1337 at 731.631 kb on + strand, within SMa1337 at 731.700 kb on + strand, within SMa1337 at 731.700 kb on + strand, within SMa1337 at 731.703 kb on + strand, within SMa1337 at 731.703 kb on + strand, within SMa1337 at 731.734 kb on - strand, within SMa1337 at 731.829 kb on + strand, within SMa1337 at 731.829 kb on + strand, within SMa1337 at 731.830 kb on - strand, within SMa1337 at 731.830 kb on - strand, within SMa1337 at 731.830 kb on - strand, within SMa1337 at 731.830 kb on - strand, within SMa1337 at 731.928 kb on + strand, within SMa1337 at 732.006 kb on + strand, within SMa1337 at 732.007 kb on - strand, within SMa1337 at 732.016 kb on - strand, within SMa1337 at 732.016 kb on - strand, within SMa1337 at 732.089 kb on + strand, within SMa1337 at 732.171 kb on + strand at 732.171 kb on + strand, within SMa1337 at 732.172 kb on - strand, within SMa1337 at 732.172 kb on - strand, within SMa1337 at 732.227 kb on - strand, within SMa1337 at 732.271 kb on - strand, within SMa1337 at 732.310 kb on - strand, within SMa1337 at 732.396 kb on + strand, within SMa1337 at 732.450 kb on + strand, within SMa1337 at 732.451 kb on - strand, within SMa1337 at 732.466 kb on - strand, within SMa1337 at 732.466 kb on - strand, within SMa1337 at 732.546 kb on + strand at 732.546 kb on + strand at 732.546 kb on + strand at 732.547 kb on - strand at 732.552 kb on + strand at 732.631 kb on - strand at 732.648 kb on + strand at 733.001 kb on - strand, within SMa1339 at 733.057 kb on + strand, within SMa1339 at 733.213 kb on + strand, within SMa1339 at 733.213 kb on + strand, within SMa1339 at 733.294 kb on + strand, within SMa1339 at 733.294 kb on + strand, within SMa1339 at 733.294 kb on + strand, within SMa1339 at 733.295 kb on - strand, within SMa1339 at 733.295 kb on - strand, within SMa1339 at 733.385 kb on - strand, within SMa1339 at 733.421 kb on - strand, within SMa1339 at 733.421 kb on - strand, within SMa1339 at 733.421 kb on - strand, within SMa1339 at 733.519 kb on + strand, within SMa1339 at 733.520 kb on - strand, within SMa1339 at 733.618 kb on + strand at 733.618 kb on + strand at 733.619 kb on - strand at 733.619 kb on - strand at 733.619 kb on - strand at 733.622 kb on + strand at 733.623 kb on - strand at 733.623 kb on - strand at 733.623 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A control for volatile experiments remove 730,360 + +1.0 730,360 + -0.7 730,450 + SMa5030 0.20 -0.1 730,451 - SMa5030 0.20 +0.3 730,453 - SMa5030 0.21 -0.4 730,514 + SMa5030 0.30 +0.0 730,720 + SMa5030 0.61 -0.8 730,720 + SMa5030 0.61 +0.2 730,721 - SMa5030 0.61 +0.1 730,721 - SMa5030 0.61 +0.2 731,096 - -0.5 731,119 + +0.6 731,119 + -0.8 731,119 + +0.3 731,119 + -0.8 731,120 - -0.3 731,120 - -0.1 731,378 + -1.2 731,467 + -1.4 731,468 - SMa1337 0.10 -1.2 731,476 - SMa1337 0.11 -0.3 731,574 + SMa1337 0.18 -1.2 731,575 - SMa1337 0.18 -0.8 731,631 + SMa1337 0.22 -0.4 731,700 + SMa1337 0.28 -2.0 731,700 + SMa1337 0.28 +0.4 731,703 + SMa1337 0.28 -0.5 731,703 + SMa1337 0.28 -0.8 731,734 - SMa1337 0.30 +0.4 731,829 + SMa1337 0.38 -0.1 731,829 + SMa1337 0.38 -0.2 731,830 - SMa1337 0.38 +0.3 731,830 - SMa1337 0.38 -0.7 731,830 - SMa1337 0.38 -0.3 731,830 - SMa1337 0.38 +0.3 731,928 + SMa1337 0.45 -1.1 732,006 + SMa1337 0.51 +0.7 732,007 - SMa1337 0.51 -0.6 732,016 - SMa1337 0.52 -0.1 732,016 - SMa1337 0.52 +0.8 732,089 + SMa1337 0.57 -0.1 732,171 + +0.8 732,171 + SMa1337 0.64 -2.0 732,172 - SMa1337 0.64 -2.2 732,172 - SMa1337 0.64 +0.3 732,227 - SMa1337 0.68 +0.8 732,271 - SMa1337 0.71 +0.2 732,310 - SMa1337 0.74 -1.6 732,396 + SMa1337 0.81 +0.7 732,450 + SMa1337 0.85 +0.5 732,451 - SMa1337 0.85 -0.5 732,466 - SMa1337 0.86 -0.4 732,466 - SMa1337 0.86 -0.0 732,546 + +0.8 732,546 + -1.8 732,546 + -0.2 732,547 - -1.3 732,552 + +0.6 732,631 - +0.7 732,648 + +0.1 733,001 - SMa1339 0.24 -1.3 733,057 + SMa1339 0.30 -0.3 733,213 + SMa1339 0.47 -1.7 733,213 + SMa1339 0.47 -0.3 733,294 + SMa1339 0.56 -0.6 733,294 + SMa1339 0.56 +0.1 733,294 + SMa1339 0.56 -0.2 733,295 - SMa1339 0.56 -0.6 733,295 - SMa1339 0.56 -0.2 733,385 - SMa1339 0.66 +0.7 733,421 - SMa1339 0.70 +1.5 733,421 - SMa1339 0.70 -0.4 733,421 - SMa1339 0.70 -0.5 733,519 + SMa1339 0.81 -0.3 733,520 - SMa1339 0.81 -0.0 733,618 + -2.0 733,618 + +0.4 733,619 - -2.2 733,619 - +0.1 733,619 - +0.2 733,622 + -1.2 733,623 - -0.6 733,623 - -0.4 733,623 - -0.5
Or see this region's nucleotide sequence