Experiment: Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS34635 and BPHYT_RS34640 are separated by 13 nucleotides BPHYT_RS34640 and BPHYT_RS34645 are separated by 108 nucleotides BPHYT_RS34645 and BPHYT_RS34650 are separated by 97 nucleotides
BPHYT_RS34635: BPHYT_RS34635 - 50S ribosomal protein L21, at 3,378,788 to 3,379,267
_RS34635
BPHYT_RS34640: BPHYT_RS34640 - TetR family transcriptional regulator, at 3,379,281 to 3,379,946
_RS34640
BPHYT_RS34645: BPHYT_RS34645 - hypothetical protein, at 3,380,055 to 3,380,705
_RS34645
BPHYT_RS34650: BPHYT_RS34650 - l-threonine 3-dehydrogenase, at 3,380,803 to 3,381,834
_RS34650
Position (kb)
3380
3381 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 3379.270 kb on + strand at 3379.271 kb on - strand at 3379.793 kb on - strand, within BPHYT_RS34640 at 3379.865 kb on - strand, within BPHYT_RS34640 at 3379.948 kb on + strand at 3379.949 kb on - strand at 3380.019 kb on + strand at 3380.020 kb on - strand at 3380.020 kb on - strand at 3380.148 kb on - strand, within BPHYT_RS34645 at 3380.148 kb on - strand, within BPHYT_RS34645 at 3380.154 kb on - strand, within BPHYT_RS34645 at 3380.247 kb on + strand, within BPHYT_RS34645 at 3380.248 kb on - strand, within BPHYT_RS34645 at 3380.635 kb on - strand, within BPHYT_RS34645 at 3380.654 kb on + strand at 3380.800 kb on - strand at 3381.094 kb on - strand, within BPHYT_RS34650 at 3381.094 kb on - strand, within BPHYT_RS34650 at 3381.240 kb on - strand, within BPHYT_RS34650 at 3381.642 kb on - strand, within BPHYT_RS34650
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days remove 3,379,270 + +0.4 3,379,271 - +0.5 3,379,793 - BPHYT_RS34640 0.77 +5.3 3,379,865 - BPHYT_RS34640 0.88 -1.5 3,379,948 + +0.2 3,379,949 - +1.2 3,380,019 + -0.5 3,380,020 - +0.8 3,380,020 - +0.9 3,380,148 - BPHYT_RS34645 0.14 -0.2 3,380,148 - BPHYT_RS34645 0.14 -0.2 3,380,154 - BPHYT_RS34645 0.15 -1.6 3,380,247 + BPHYT_RS34645 0.29 -0.9 3,380,248 - BPHYT_RS34645 0.30 -2.1 3,380,635 - BPHYT_RS34645 0.89 -0.7 3,380,654 + -1.0 3,380,800 - +1.6 3,381,094 - BPHYT_RS34650 0.28 -0.5 3,381,094 - BPHYT_RS34650 0.28 -1.9 3,381,240 - BPHYT_RS34650 0.42 +0.5 3,381,642 - BPHYT_RS34650 0.81 +1.0
Or see this region's nucleotide sequence