Experiment: Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS32160 and BPHYT_RS32165 overlap by 8 nucleotides BPHYT_RS32165 and BPHYT_RS32170 overlap by 4 nucleotides BPHYT_RS32170 and BPHYT_RS32175 are separated by 183 nucleotides
BPHYT_RS32160: BPHYT_RS32160 - type IV secretion system protein VirB10, at 2,788,418 to 2,789,662
_RS32160
BPHYT_RS32165: BPHYT_RS32165 - type IV secretion system protein VirB9, at 2,789,655 to 2,790,485
_RS32165
BPHYT_RS32170: BPHYT_RS32170 - conjugal transfer protein TraJ, at 2,790,482 to 2,791,186
_RS32170
BPHYT_RS32175: BPHYT_RS32175 - conjugal transfer protein TraF, at 2,791,370 to 2,792,065
_RS32175
Position (kb)
2789
2790
2791 Strain fitness (log2 ratio)
-2
-1
0
1 at 2788.833 kb on + strand, within BPHYT_RS32160 at 2789.235 kb on + strand, within BPHYT_RS32160 at 2789.238 kb on + strand, within BPHYT_RS32160 at 2789.238 kb on + strand, within BPHYT_RS32160 at 2789.737 kb on + strand at 2789.738 kb on - strand, within BPHYT_RS32165 at 2789.782 kb on - strand, within BPHYT_RS32165 at 2790.099 kb on + strand, within BPHYT_RS32165 at 2790.100 kb on - strand, within BPHYT_RS32165 at 2790.367 kb on + strand, within BPHYT_RS32165 at 2790.524 kb on - strand at 2790.618 kb on + strand, within BPHYT_RS32170 at 2790.801 kb on + strand, within BPHYT_RS32170 at 2790.802 kb on - strand, within BPHYT_RS32170 at 2790.814 kb on - strand, within BPHYT_RS32170 at 2790.907 kb on - strand, within BPHYT_RS32170
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days remove 2,788,833 + BPHYT_RS32160 0.33 +0.8 2,789,235 + BPHYT_RS32160 0.66 +0.7 2,789,238 + BPHYT_RS32160 0.66 -1.3 2,789,238 + BPHYT_RS32160 0.66 -2.4 2,789,737 + -1.1 2,789,738 - BPHYT_RS32165 0.10 -0.1 2,789,782 - BPHYT_RS32165 0.15 -0.2 2,790,099 + BPHYT_RS32165 0.53 +1.2 2,790,100 - BPHYT_RS32165 0.54 +1.1 2,790,367 + BPHYT_RS32165 0.86 -1.7 2,790,524 - -1.0 2,790,618 + BPHYT_RS32170 0.19 -0.6 2,790,801 + BPHYT_RS32170 0.45 +1.2 2,790,802 - BPHYT_RS32170 0.45 -1.2 2,790,814 - BPHYT_RS32170 0.47 +0.2 2,790,907 - BPHYT_RS32170 0.60 +0.0
Or see this region's nucleotide sequence