Experiment: Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS31420 and BPHYT_RS31425 are separated by 33 nucleotides BPHYT_RS31425 and BPHYT_RS31430 are separated by 52 nucleotides BPHYT_RS31430 and BPHYT_RS31435 are separated by 149 nucleotides
BPHYT_RS31420: BPHYT_RS31420 - ABC transporter substrate-binding protein, at 2,603,846 to 2,604,658
_RS31420
BPHYT_RS31425: BPHYT_RS31425 - arginine ABC transporter ATP-binding protein, at 2,604,692 to 2,605,393
_RS31425
BPHYT_RS31430: BPHYT_RS31430 - FAD-dependent oxidoreductase, at 2,605,446 to 2,606,771
_RS31430
BPHYT_RS31435: BPHYT_RS31435 - LysR family transcriptional regulator, at 2,606,921 to 2,607,823
_RS31435
Position (kb)
2605
2606
2607 Strain fitness (log2 ratio)
-2
-1
0
1 at 2604.650 kb on + strand at 2604.996 kb on + strand, within BPHYT_RS31425 at 2605.363 kb on - strand at 2605.428 kb on - strand at 2605.602 kb on - strand, within BPHYT_RS31430 at 2606.240 kb on - strand, within BPHYT_RS31430 at 2606.361 kb on - strand, within BPHYT_RS31430
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days remove 2,604,650 + -0.8 2,604,996 + BPHYT_RS31425 0.43 -2.1 2,605,363 - -2.1 2,605,428 - -2.0 2,605,602 - BPHYT_RS31430 0.12 -2.2 2,606,240 - BPHYT_RS31430 0.60 -1.2 2,606,361 - BPHYT_RS31430 0.69 -0.6
Or see this region's nucleotide sequence