Experiment: Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS29585 and BPHYT_RS29590 are separated by 103 nucleotides BPHYT_RS29590 and BPHYT_RS29595 are separated by 748 nucleotides
BPHYT_RS29585: BPHYT_RS29585 - hypothetical protein, at 2,190,730 to 2,191,821
_RS29585
BPHYT_RS29590: BPHYT_RS29590 - glycogen phosphorylase, at 2,191,925 to 2,194,378
_RS29590
BPHYT_RS29595: BPHYT_RS29595 - MFS transporter, at 2,195,127 to 2,196,494
_RS29595
Position (kb)
2191
2192
2193
2194
2195 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2191.413 kb on + strand, within BPHYT_RS29585 at 2191.624 kb on + strand, within BPHYT_RS29585 at 2191.889 kb on - strand at 2191.895 kb on - strand at 2192.116 kb on - strand at 2192.150 kb on - strand at 2192.290 kb on + strand, within BPHYT_RS29590 at 2192.670 kb on + strand, within BPHYT_RS29590 at 2192.713 kb on - strand, within BPHYT_RS29590 at 2192.972 kb on - strand, within BPHYT_RS29590 at 2193.103 kb on - strand, within BPHYT_RS29590 at 2193.413 kb on - strand, within BPHYT_RS29590 at 2193.413 kb on - strand, within BPHYT_RS29590 at 2193.804 kb on + strand, within BPHYT_RS29590 at 2193.813 kb on + strand, within BPHYT_RS29590 at 2193.813 kb on + strand, within BPHYT_RS29590 at 2193.813 kb on + strand, within BPHYT_RS29590 at 2193.814 kb on - strand, within BPHYT_RS29590 at 2193.814 kb on - strand, within BPHYT_RS29590 at 2193.864 kb on + strand, within BPHYT_RS29590 at 2193.864 kb on + strand, within BPHYT_RS29590 at 2194.120 kb on + strand, within BPHYT_RS29590 at 2194.155 kb on + strand at 2194.156 kb on - strand at 2194.159 kb on - strand at 2194.159 kb on - strand at 2194.296 kb on + strand at 2194.297 kb on - strand at 2194.297 kb on - strand at 2194.340 kb on - strand at 2194.407 kb on + strand at 2194.688 kb on + strand at 2194.859 kb on + strand at 2194.859 kb on + strand at 2194.860 kb on - strand at 2194.860 kb on - strand at 2194.919 kb on + strand at 2195.278 kb on - strand, within BPHYT_RS29595 at 2195.349 kb on + strand, within BPHYT_RS29595 at 2195.349 kb on + strand, within BPHYT_RS29595 at 2195.350 kb on - strand, within BPHYT_RS29595 at 2195.350 kb on - strand, within BPHYT_RS29595 at 2195.350 kb on - strand, within BPHYT_RS29595
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days remove 2,191,413 + BPHYT_RS29585 0.63 +0.6 2,191,624 + BPHYT_RS29585 0.82 +1.4 2,191,889 - +0.7 2,191,895 - -1.3 2,192,116 - -1.1 2,192,150 - -0.4 2,192,290 + BPHYT_RS29590 0.15 -1.9 2,192,670 + BPHYT_RS29590 0.30 -0.2 2,192,713 - BPHYT_RS29590 0.32 -2.4 2,192,972 - BPHYT_RS29590 0.43 +0.6 2,193,103 - BPHYT_RS29590 0.48 -0.1 2,193,413 - BPHYT_RS29590 0.61 +0.6 2,193,413 - BPHYT_RS29590 0.61 -0.6 2,193,804 + BPHYT_RS29590 0.77 +0.2 2,193,813 + BPHYT_RS29590 0.77 -0.4 2,193,813 + BPHYT_RS29590 0.77 +0.7 2,193,813 + BPHYT_RS29590 0.77 +0.2 2,193,814 - BPHYT_RS29590 0.77 -0.3 2,193,814 - BPHYT_RS29590 0.77 -1.9 2,193,864 + BPHYT_RS29590 0.79 -0.7 2,193,864 + BPHYT_RS29590 0.79 -1.8 2,194,120 + BPHYT_RS29590 0.89 +0.2 2,194,155 + -0.3 2,194,156 - -2.5 2,194,159 - -0.0 2,194,159 - -0.3 2,194,296 + -0.8 2,194,297 - -1.4 2,194,297 - -1.4 2,194,340 - -0.6 2,194,407 + -3.9 2,194,688 + +0.1 2,194,859 + +0.3 2,194,859 + -1.1 2,194,860 - -1.8 2,194,860 - -0.8 2,194,919 + +0.5 2,195,278 - BPHYT_RS29595 0.11 +0.5 2,195,349 + BPHYT_RS29595 0.16 -2.7 2,195,349 + BPHYT_RS29595 0.16 +1.3 2,195,350 - BPHYT_RS29595 0.16 -0.4 2,195,350 - BPHYT_RS29595 0.16 +0.2 2,195,350 - BPHYT_RS29595 0.16 -2.6
Or see this region's nucleotide sequence