Experiment: Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS29220 and BPHYT_RS29225 are separated by 186 nucleotides BPHYT_RS29225 and BPHYT_RS29230 are separated by 126 nucleotides BPHYT_RS29230 and BPHYT_RS29235 are separated by 24 nucleotides
BPHYT_RS29220: BPHYT_RS29220 - hypothetical protein, at 2,111,904 to 2,112,644
_RS29220
BPHYT_RS29225: BPHYT_RS29225 - diguanylate phosphodiesterase, at 2,112,831 to 2,114,900
_RS29225
BPHYT_RS29230: BPHYT_RS29230 - hypothetical protein, at 2,115,027 to 2,115,833
_RS29230
BPHYT_RS29235: BPHYT_RS29235 - cell shape determination protein CcmA, at 2,115,858 to 2,116,292
_RS29235
Position (kb)
2112
2113
2114
2115 Strain fitness (log2 ratio)
-1
0
1
2 at 2112.160 kb on + strand, within BPHYT_RS29220 at 2112.207 kb on - strand, within BPHYT_RS29220 at 2112.208 kb on + strand, within BPHYT_RS29220 at 2112.252 kb on - strand, within BPHYT_RS29220 at 2112.458 kb on - strand, within BPHYT_RS29220 at 2112.560 kb on - strand, within BPHYT_RS29220 at 2112.578 kb on - strand at 2112.772 kb on - strand at 2112.829 kb on - strand at 2112.894 kb on + strand at 2113.101 kb on + strand, within BPHYT_RS29225 at 2113.114 kb on - strand, within BPHYT_RS29225 at 2113.349 kb on + strand, within BPHYT_RS29225 at 2113.401 kb on + strand, within BPHYT_RS29225 at 2114.050 kb on - strand, within BPHYT_RS29225 at 2114.050 kb on - strand, within BPHYT_RS29225 at 2114.999 kb on - strand at 2115.040 kb on + strand at 2115.436 kb on - strand, within BPHYT_RS29230 at 2115.442 kb on + strand, within BPHYT_RS29230 at 2115.442 kb on + strand, within BPHYT_RS29230
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days remove 2,112,160 + BPHYT_RS29220 0.35 +0.3 2,112,207 - BPHYT_RS29220 0.41 +0.1 2,112,208 + BPHYT_RS29220 0.41 -1.0 2,112,252 - BPHYT_RS29220 0.47 -0.4 2,112,458 - BPHYT_RS29220 0.75 +0.1 2,112,560 - BPHYT_RS29220 0.89 -0.4 2,112,578 - -1.0 2,112,772 - +0.7 2,112,829 - +0.1 2,112,894 + -0.5 2,113,101 + BPHYT_RS29225 0.13 +0.2 2,113,114 - BPHYT_RS29225 0.14 +0.1 2,113,349 + BPHYT_RS29225 0.25 -0.3 2,113,401 + BPHYT_RS29225 0.28 +2.1 2,114,050 - BPHYT_RS29225 0.59 +0.4 2,114,050 - BPHYT_RS29225 0.59 +0.4 2,114,999 - -1.6 2,115,040 + +0.1 2,115,436 - BPHYT_RS29230 0.51 +1.0 2,115,442 + BPHYT_RS29230 0.51 +0.6 2,115,442 + BPHYT_RS29230 0.51 +0.0
Or see this region's nucleotide sequence