Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS28735

Experiment: Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS28730 and BPHYT_RS28735 are separated by 160 nucleotidesBPHYT_RS28735 and BPHYT_RS28740 are separated by 29 nucleotidesBPHYT_RS28740 and BPHYT_RS28745 are separated by 13 nucleotides BPHYT_RS28730: BPHYT_RS28730 - galactarate dehydratase, at 1,999,039 to 2,000,550 _RS28730 BPHYT_RS28735: BPHYT_RS28735 - hypothetical protein, at 2,000,711 to 2,001,406 _RS28735 BPHYT_RS28740: BPHYT_RS28740 - 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, at 2,001,436 to 2,002,326 _RS28740 BPHYT_RS28745: BPHYT_RS28745 - 2-keto-4-pentenoate hydratase, at 2,002,340 to 2,003,149 _RS28745 Position (kb) 2000 2001 2002Strain fitness (log2 ratio) -2 -1 0 1at 1999.719 kb on + strand, within BPHYT_RS28730at 2000.202 kb on - strand, within BPHYT_RS28730at 2000.341 kb on + strand, within BPHYT_RS28730at 2000.342 kb on - strand, within BPHYT_RS28730at 2000.817 kb on - strand, within BPHYT_RS28735at 2000.878 kb on + strand, within BPHYT_RS28735at 2000.879 kb on - strand, within BPHYT_RS28735at 2001.073 kb on - strand, within BPHYT_RS28735at 2001.077 kb on - strand, within BPHYT_RS28735at 2001.077 kb on - strand, within BPHYT_RS28735at 2001.406 kb on + strandat 2001.438 kb on - strandat 2001.480 kb on + strandat 2001.489 kb on - strandat 2001.609 kb on + strand, within BPHYT_RS28740at 2001.651 kb on - strand, within BPHYT_RS28740at 2001.810 kb on + strand, within BPHYT_RS28740

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days
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1,999,719 + BPHYT_RS28730 0.45 -0.1
2,000,202 - BPHYT_RS28730 0.77 -0.0
2,000,341 + BPHYT_RS28730 0.86 +0.4
2,000,342 - BPHYT_RS28730 0.86 +0.3
2,000,817 - BPHYT_RS28735 0.15 -1.6
2,000,878 + BPHYT_RS28735 0.24 +0.7
2,000,879 - BPHYT_RS28735 0.24 +0.3
2,001,073 - BPHYT_RS28735 0.52 +1.3
2,001,077 - BPHYT_RS28735 0.53 -1.1
2,001,077 - BPHYT_RS28735 0.53 +1.5
2,001,406 + +0.8
2,001,438 - -1.1
2,001,480 + -0.7
2,001,489 - +1.7
2,001,609 + BPHYT_RS28740 0.19 -0.5
2,001,651 - BPHYT_RS28740 0.24 +1.3
2,001,810 + BPHYT_RS28740 0.42 -2.1

Or see this region's nucleotide sequence