Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS28335

Experiment: Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS28325 and BPHYT_RS28330 are separated by 323 nucleotidesBPHYT_RS28330 and BPHYT_RS28335 are separated by 134 nucleotidesBPHYT_RS28335 and BPHYT_RS28340 overlap by 20 nucleotides BPHYT_RS28325: BPHYT_RS28325 - NADPH-dependent FMN reductase, at 1,908,365 to 1,909,024 _RS28325 BPHYT_RS28330: BPHYT_RS28330 - transcriptional regulator, at 1,909,348 to 1,909,563 _RS28330 BPHYT_RS28335: BPHYT_RS28335 - hypothetical protein, at 1,909,698 to 1,910,651 _RS28335 BPHYT_RS28340: BPHYT_RS28340 - indole acetimide hydrolase, at 1,910,632 to 1,912,086 _RS28340 Position (kb) 1909 1910 1911Strain fitness (log2 ratio) -1 0 1 2 3at 1908.701 kb on + strand, within BPHYT_RS28325at 1909.022 kb on + strandat 1909.023 kb on - strandat 1909.360 kb on - strandat 1909.519 kb on + strand, within BPHYT_RS28330at 1909.561 kb on + strandat 1909.688 kb on + strandat 1909.836 kb on + strand, within BPHYT_RS28335at 1909.956 kb on - strand, within BPHYT_RS28335at 1910.091 kb on + strand, within BPHYT_RS28335at 1910.137 kb on - strand, within BPHYT_RS28335at 1910.137 kb on - strand, within BPHYT_RS28335at 1910.139 kb on - strand, within BPHYT_RS28335at 1911.013 kb on - strand, within BPHYT_RS28340at 1911.259 kb on + strand, within BPHYT_RS28340at 1911.294 kb on + strand, within BPHYT_RS28340at 1911.356 kb on + strand, within BPHYT_RS28340at 1911.357 kb on - strand, within BPHYT_RS28340

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days
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1,908,701 + BPHYT_RS28325 0.51 +0.3
1,909,022 + +2.8
1,909,023 - +1.3
1,909,360 - +0.6
1,909,519 + BPHYT_RS28330 0.79 -1.4
1,909,561 + -0.6
1,909,688 + -0.1
1,909,836 + BPHYT_RS28335 0.14 -0.0
1,909,956 - BPHYT_RS28335 0.27 +0.6
1,910,091 + BPHYT_RS28335 0.41 -0.2
1,910,137 - BPHYT_RS28335 0.46 +0.1
1,910,137 - BPHYT_RS28335 0.46 -0.1
1,910,139 - BPHYT_RS28335 0.46 -1.0
1,911,013 - BPHYT_RS28340 0.26 -1.3
1,911,259 + BPHYT_RS28340 0.43 -0.9
1,911,294 + BPHYT_RS28340 0.45 -0.1
1,911,356 + BPHYT_RS28340 0.50 +1.0
1,911,357 - BPHYT_RS28340 0.50 -0.9

Or see this region's nucleotide sequence