Experiment: Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22925 and BPHYT_RS22930 are separated by 43 nucleotides BPHYT_RS22930 and BPHYT_RS22935 are separated by 26 nucleotides BPHYT_RS22935 and BPHYT_RS22940 overlap by 4 nucleotides
BPHYT_RS22925: BPHYT_RS22925 - pilus assembly protein TadB, at 702,385 to 703,362
_RS22925
BPHYT_RS22930: BPHYT_RS22930 - type II secretion system protein, at 703,406 to 704,362
_RS22930
BPHYT_RS22935: BPHYT_RS22935 - fatty acid hydroxylase, at 704,389 to 705,255
_RS22935
BPHYT_RS22940: BPHYT_RS22940 - pilus assembly protein TadE, at 705,252 to 705,782
_RS22940
Position (kb) 703
704
705 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 702.688 kb on + strand, within BPHYT_RS22925 at 702.689 kb on - strand, within BPHYT_RS22925 at 702.689 kb on - strand, within BPHYT_RS22925 at 702.930 kb on - strand, within BPHYT_RS22925 at 703.835 kb on + strand, within BPHYT_RS22930 at 704.175 kb on - strand, within BPHYT_RS22930 at 704.374 kb on - strand at 704.563 kb on + strand, within BPHYT_RS22935 at 704.563 kb on + strand, within BPHYT_RS22935 at 704.742 kb on + strand, within BPHYT_RS22935 at 704.743 kb on - strand, within BPHYT_RS22935 at 704.743 kb on - strand, within BPHYT_RS22935 at 704.743 kb on - strand, within BPHYT_RS22935 at 704.857 kb on + strand, within BPHYT_RS22935 at 704.936 kb on - strand, within BPHYT_RS22935
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days remove 702,688 + BPHYT_RS22925 0.31 +0.4 702,689 - BPHYT_RS22925 0.31 +0.3 702,689 - BPHYT_RS22925 0.31 +1.0 702,930 - BPHYT_RS22925 0.56 +0.9 703,835 + BPHYT_RS22930 0.45 -3.4 704,175 - BPHYT_RS22930 0.80 +2.4 704,374 - +0.1 704,563 + BPHYT_RS22935 0.20 +0.5 704,563 + BPHYT_RS22935 0.20 +1.4 704,742 + BPHYT_RS22935 0.41 -0.0 704,743 - BPHYT_RS22935 0.41 +1.6 704,743 - BPHYT_RS22935 0.41 -0.1 704,743 - BPHYT_RS22935 0.41 +1.1 704,857 + BPHYT_RS22935 0.54 +0.4 704,936 - BPHYT_RS22935 0.63 +0.2
Or see this region's nucleotide sequence