Experiment: Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21775 and BPHYT_RS21780 are separated by 63 nucleotides BPHYT_RS21780 and BPHYT_RS21785 are separated by 439 nucleotides BPHYT_RS21785 and BPHYT_RS21790 are separated by 88 nucleotides
BPHYT_RS21775: BPHYT_RS21775 - long-chain fatty acid--CoA ligase, at 441,277 to 442,938
_RS21775
BPHYT_RS21780: BPHYT_RS21780 - MFS transporter, at 443,002 to 444,384
_RS21780
BPHYT_RS21785: BPHYT_RS21785 - hypothetical protein, at 444,824 to 445,156
_RS21785
BPHYT_RS21790: BPHYT_RS21790 - hypothetical protein, at 445,245 to 445,583
_RS21790
Position (kb) 443
444
445 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 442.199 kb on - strand, within BPHYT_RS21775 at 442.519 kb on + strand, within BPHYT_RS21775 at 442.982 kb on + strand at 442.983 kb on - strand at 443.037 kb on + strand at 443.038 kb on - strand at 443.876 kb on - strand, within BPHYT_RS21780 at 444.074 kb on - strand, within BPHYT_RS21780 at 444.189 kb on + strand, within BPHYT_RS21780 at 444.539 kb on + strand at 444.673 kb on - strand at 445.117 kb on + strand, within BPHYT_RS21785 at 445.146 kb on - strand at 445.289 kb on + strand, within BPHYT_RS21790 at 445.290 kb on - strand, within BPHYT_RS21790
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days remove 442,199 - BPHYT_RS21775 0.55 +1.0 442,519 + BPHYT_RS21775 0.75 +0.6 442,982 + -1.5 442,983 - +0.0 443,037 + -0.8 443,038 - -1.8 443,876 - BPHYT_RS21780 0.63 +2.0 444,074 - BPHYT_RS21780 0.78 -0.8 444,189 + BPHYT_RS21780 0.86 +0.4 444,539 + -0.7 444,673 - -0.4 445,117 + BPHYT_RS21785 0.88 +0.8 445,146 - -3.2 445,289 + BPHYT_RS21790 0.13 +0.7 445,290 - BPHYT_RS21790 0.13 +0.5
Or see this region's nucleotide sequence