Experiment: Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21690 and BPHYT_RS21695 are separated by 21 nucleotides BPHYT_RS21695 and BPHYT_RS21700 overlap by 4 nucleotides BPHYT_RS21700 and BPHYT_RS21705 are separated by 148 nucleotides
BPHYT_RS21690: BPHYT_RS21690 - cobalt chelatase, at 420,858 to 422,591
_RS21690
BPHYT_RS21695: BPHYT_RS21695 - membrane protein, at 422,613 to 423,374
_RS21695
BPHYT_RS21700: BPHYT_RS21700 - phosphotransacetylase, at 423,371 to 424,405
_RS21700
BPHYT_RS21705: BPHYT_RS21705 - sulfoacetaldehyde acetyltransferase, at 424,554 to 426,386
_RS21705
Position (kb) 423
424
425 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3
4 at 422.400 kb on + strand, within BPHYT_RS21690 at 422.400 kb on + strand, within BPHYT_RS21690 at 422.403 kb on + strand at 422.403 kb on + strand, within BPHYT_RS21690 at 422.404 kb on - strand, within BPHYT_RS21690 at 422.499 kb on + strand at 422.500 kb on - strand at 422.896 kb on + strand, within BPHYT_RS21695 at 422.896 kb on + strand, within BPHYT_RS21695 at 423.037 kb on + strand, within BPHYT_RS21695 at 423.376 kb on + strand at 423.376 kb on + strand at 423.376 kb on + strand at 423.450 kb on + strand at 424.189 kb on + strand, within BPHYT_RS21700 at 424.190 kb on - strand, within BPHYT_RS21700 at 424.406 kb on - strand at 424.561 kb on + strand at 424.562 kb on - strand at 424.570 kb on + strand at 424.813 kb on + strand, within BPHYT_RS21705 at 424.945 kb on + strand, within BPHYT_RS21705 at 424.946 kb on - strand, within BPHYT_RS21705 at 425.065 kb on + strand, within BPHYT_RS21705 at 425.167 kb on + strand, within BPHYT_RS21705
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days remove 422,400 + BPHYT_RS21690 0.89 -0.6 422,400 + BPHYT_RS21690 0.89 -1.3 422,403 + -0.8 422,403 + BPHYT_RS21690 0.89 -0.8 422,404 - BPHYT_RS21690 0.89 +0.1 422,499 + -0.5 422,500 - +0.3 422,896 + BPHYT_RS21695 0.37 -0.2 422,896 + BPHYT_RS21695 0.37 -0.7 423,037 + BPHYT_RS21695 0.56 -0.5 423,376 + +0.5 423,376 + +3.9 423,376 + -0.4 423,450 + -1.4 424,189 + BPHYT_RS21700 0.79 -0.1 424,190 - BPHYT_RS21700 0.79 -2.8 424,406 - +1.8 424,561 + -4.1 424,562 - -1.8 424,570 + -6.2 424,813 + BPHYT_RS21705 0.14 -0.9 424,945 + BPHYT_RS21705 0.21 -0.4 424,946 - BPHYT_RS21705 0.21 -2.8 425,065 + BPHYT_RS21705 0.28 -3.3 425,167 + BPHYT_RS21705 0.33 -4.1
Or see this region's nucleotide sequence