Experiment: Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21490 and BPHYT_RS21495 overlap by 4 nucleotides BPHYT_RS21495 and BPHYT_RS21500 overlap by 4 nucleotides BPHYT_RS21500 and BPHYT_RS21505 are separated by 78 nucleotides
BPHYT_RS21490: BPHYT_RS21490 - ABC transporter permease, at 380,697 to 382,637
_RS21490
BPHYT_RS21495: BPHYT_RS21495 - ABC transporter, at 382,634 to 383,443
_RS21495
BPHYT_RS21500: BPHYT_RS21500 - ABC transporter, at 383,440 to 384,153
_RS21500
BPHYT_RS21505: BPHYT_RS21505 - amidohydrolase, at 384,232 to 385,431
_RS21505
Position (kb)
382
383
384 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 381.853 kb on - strand, within BPHYT_RS21490 at 381.979 kb on - strand, within BPHYT_RS21490 at 382.113 kb on + strand, within BPHYT_RS21490 at 382.116 kb on + strand, within BPHYT_RS21490 at 382.116 kb on + strand, within BPHYT_RS21490 at 382.474 kb on - strand at 382.633 kb on + strand at 383.033 kb on - strand, within BPHYT_RS21495 at 383.496 kb on + strand at 384.061 kb on + strand, within BPHYT_RS21500
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days remove 381,853 - BPHYT_RS21490 0.60 +1.2 381,979 - BPHYT_RS21490 0.66 +0.2 382,113 + BPHYT_RS21490 0.73 +1.3 382,116 + BPHYT_RS21490 0.73 +0.3 382,116 + BPHYT_RS21490 0.73 -2.3 382,474 - -2.0 382,633 + -0.9 383,033 - BPHYT_RS21495 0.49 -3.6 383,496 + +0.2 384,061 + BPHYT_RS21500 0.87 -1.1
Or see this region's nucleotide sequence