Experiment: Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20665 and BPHYT_RS20670 are separated by 92 nucleotides BPHYT_RS20670 and BPHYT_RS20675 are separated by 30 nucleotides
BPHYT_RS20665: BPHYT_RS20665 - LysR family transcriptional regulator, at 196,631 to 197,530
_RS20665
BPHYT_RS20670: BPHYT_RS20670 - carbon-nitrogen hydrolase, at 197,623 to 198,579
_RS20670
BPHYT_RS20675: BPHYT_RS20675 - LysR family transcriptional regulator, at 198,610 to 199,482
_RS20675
Position (kb) 197
198
199 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 197.057 kb on + strand, within BPHYT_RS20665 at 197.785 kb on + strand, within BPHYT_RS20670 at 198.094 kb on + strand, within BPHYT_RS20670 at 198.094 kb on + strand, within BPHYT_RS20670 at 198.290 kb on + strand, within BPHYT_RS20670 at 198.290 kb on + strand, within BPHYT_RS20670 at 198.348 kb on - strand, within BPHYT_RS20670 at 198.457 kb on + strand, within BPHYT_RS20670 at 198.464 kb on + strand, within BPHYT_RS20670 at 198.555 kb on + strand at 198.962 kb on + strand, within BPHYT_RS20675 at 198.962 kb on + strand, within BPHYT_RS20675 at 199.016 kb on + strand, within BPHYT_RS20675 at 199.016 kb on + strand, within BPHYT_RS20675 at 199.082 kb on + strand, within BPHYT_RS20675 at 199.163 kb on + strand, within BPHYT_RS20675 at 199.241 kb on - strand, within BPHYT_RS20675 at 199.537 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days remove 197,057 + BPHYT_RS20665 0.47 +3.5 197,785 + BPHYT_RS20670 0.17 -0.6 198,094 + BPHYT_RS20670 0.49 -1.4 198,094 + BPHYT_RS20670 0.49 -0.6 198,290 + BPHYT_RS20670 0.70 -0.3 198,290 + BPHYT_RS20670 0.70 -2.7 198,348 - BPHYT_RS20670 0.76 -0.4 198,457 + BPHYT_RS20670 0.87 -0.1 198,464 + BPHYT_RS20670 0.88 -1.6 198,555 + -0.9 198,962 + BPHYT_RS20675 0.40 -0.2 198,962 + BPHYT_RS20675 0.40 +0.3 199,016 + BPHYT_RS20675 0.47 -1.3 199,016 + BPHYT_RS20675 0.47 -1.5 199,082 + BPHYT_RS20675 0.54 +0.1 199,163 + BPHYT_RS20675 0.63 -1.2 199,241 - BPHYT_RS20675 0.72 +0.3 199,537 - +1.1
Or see this region's nucleotide sequence