Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS18750

Experiment: Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS18745 and BPHYT_RS18750 are separated by 3 nucleotidesBPHYT_RS18750 and BPHYT_RS18755 overlap by 1 nucleotides BPHYT_RS18745: BPHYT_RS18745 - coniferyl aldehyde dehydrogenase, at 4,216,245 to 4,217,663 _RS18745 BPHYT_RS18750: BPHYT_RS18750 - flagellar biosynthetic protein FlhB, at 4,217,667 to 4,218,026 _RS18750 BPHYT_RS18755: BPHYT_RS18755 - flagellar hook-length control protein FliK, at 4,218,026 to 4,219,459 _RS18755 Position (kb) 4217 4218 4219Strain fitness (log2 ratio) -2 -1 0 1at 4217.083 kb on - strand, within BPHYT_RS18745at 4217.319 kb on + strand, within BPHYT_RS18745at 4217.319 kb on + strand, within BPHYT_RS18745at 4217.319 kb on + strand, within BPHYT_RS18745at 4217.320 kb on - strand, within BPHYT_RS18745at 4217.379 kb on + strand, within BPHYT_RS18745at 4217.379 kb on + strand, within BPHYT_RS18745at 4217.380 kb on - strand, within BPHYT_RS18745at 4217.385 kb on + strand, within BPHYT_RS18745at 4217.523 kb on + strandat 4217.524 kb on - strandat 4217.813 kb on - strand, within BPHYT_RS18750at 4217.813 kb on - strand, within BPHYT_RS18750at 4217.887 kb on + strand, within BPHYT_RS18750at 4217.887 kb on + strand, within BPHYT_RS18750at 4217.887 kb on + strand, within BPHYT_RS18750at 4217.939 kb on - strand, within BPHYT_RS18750at 4217.987 kb on - strand, within BPHYT_RS18750at 4217.987 kb on - strand, within BPHYT_RS18750at 4218.157 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days
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4,217,083 - BPHYT_RS18745 0.59 +0.2
4,217,319 + BPHYT_RS18745 0.76 +1.7
4,217,319 + BPHYT_RS18745 0.76 +0.4
4,217,319 + BPHYT_RS18745 0.76 -0.2
4,217,320 - BPHYT_RS18745 0.76 +0.5
4,217,379 + BPHYT_RS18745 0.80 -1.4
4,217,379 + BPHYT_RS18745 0.80 -0.9
4,217,380 - BPHYT_RS18745 0.80 +1.1
4,217,385 + BPHYT_RS18745 0.80 +0.2
4,217,523 + +0.3
4,217,524 - -0.6
4,217,813 - BPHYT_RS18750 0.41 +0.1
4,217,813 - BPHYT_RS18750 0.41 -0.2
4,217,887 + BPHYT_RS18750 0.61 +1.0
4,217,887 + BPHYT_RS18750 0.61 +1.6
4,217,887 + BPHYT_RS18750 0.61 -2.4
4,217,939 - BPHYT_RS18750 0.76 -0.9
4,217,987 - BPHYT_RS18750 0.89 +0.9
4,217,987 - BPHYT_RS18750 0.89 +0.6
4,218,157 - +1.0

Or see this region's nucleotide sequence