Experiment: Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS14845 and BPHYT_RS14850 are separated by 274 nucleotides BPHYT_RS14850 and BPHYT_RS14855 are separated by 77 nucleotides BPHYT_RS14855 and BPHYT_RS14860 are separated by 148 nucleotides
BPHYT_RS14845: BPHYT_RS14845 - cysteine synthase, at 3,377,128 to 3,378,030
_RS14845
BPHYT_RS14850: BPHYT_RS14850 - competence protein ComE, at 3,378,305 to 3,378,670
_RS14850
BPHYT_RS14855: BPHYT_RS14855 - ADP-L-glycero-D-manno-heptose-6-epimerase, at 3,378,748 to 3,379,740
_RS14855
BPHYT_RS14860: BPHYT_RS14860 - sugar kinase, at 3,379,889 to 3,380,809
_RS14860
Position (kb)
3378
3379
3380 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3377.759 kb on + strand, within BPHYT_RS14845 at 3377.983 kb on + strand at 3377.983 kb on + strand at 3378.030 kb on + strand at 3378.275 kb on + strand at 3378.307 kb on - strand at 3378.670 kb on + strand at 3378.670 kb on + strand at 3378.670 kb on + strand at 3378.686 kb on + strand at 3378.764 kb on - strand at 3379.212 kb on + strand, within BPHYT_RS14855 at 3379.232 kb on + strand, within BPHYT_RS14855 at 3379.590 kb on - strand, within BPHYT_RS14855
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days remove 3,377,759 + BPHYT_RS14845 0.70 -2.2 3,377,983 + -2.4 3,377,983 + -3.3 3,378,030 + -3.1 3,378,275 + +0.3 3,378,307 - +2.4 3,378,670 + +0.3 3,378,670 + +1.0 3,378,670 + -0.1 3,378,686 + +1.3 3,378,764 - +0.5 3,379,212 + BPHYT_RS14855 0.47 -2.6 3,379,232 + BPHYT_RS14855 0.49 +0.1 3,379,590 - BPHYT_RS14855 0.85 -0.9
Or see this region's nucleotide sequence