Experiment: Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS14465 and BPHYT_RS14470 are separated by 33 nucleotides BPHYT_RS14470 and BPHYT_RS14475 overlap by 4 nucleotides BPHYT_RS14475 and BPHYT_RS14480 overlap by 8 nucleotides
BPHYT_RS14465: BPHYT_RS14465 - peptidase S49, at 3,297,421 to 3,298,425
_RS14465
BPHYT_RS14470: BPHYT_RS14470 - Rieske (2Fe-2S) protein, at 3,298,459 to 3,298,863
_RS14470
BPHYT_RS14475: BPHYT_RS14475 - HAD family hydrolase, at 3,298,860 to 3,299,519
_RS14475
BPHYT_RS14480: BPHYT_RS14480 - ribosomal large subunit pseudouridine synthase C, at 3,299,512 to 3,300,537
_RS14480
Position (kb)
3298
3299 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3297.531 kb on - strand, within BPHYT_RS14465 at 3297.802 kb on - strand, within BPHYT_RS14465 at 3297.802 kb on - strand, within BPHYT_RS14465 at 3297.903 kb on - strand, within BPHYT_RS14465 at 3297.903 kb on - strand, within BPHYT_RS14465 at 3297.930 kb on - strand, within BPHYT_RS14465 at 3297.969 kb on - strand, within BPHYT_RS14465 at 3298.054 kb on - strand, within BPHYT_RS14465 at 3298.141 kb on - strand, within BPHYT_RS14465 at 3298.246 kb on + strand, within BPHYT_RS14465 at 3298.247 kb on - strand, within BPHYT_RS14465 at 3298.324 kb on - strand, within BPHYT_RS14465 at 3298.602 kb on - strand, within BPHYT_RS14470 at 3298.618 kb on - strand, within BPHYT_RS14470 at 3298.618 kb on - strand, within BPHYT_RS14470 at 3298.631 kb on + strand, within BPHYT_RS14470 at 3298.631 kb on + strand, within BPHYT_RS14470 at 3298.632 kb on - strand, within BPHYT_RS14470 at 3298.684 kb on + strand, within BPHYT_RS14470 at 3298.712 kb on + strand, within BPHYT_RS14470 at 3298.727 kb on + strand, within BPHYT_RS14470 at 3298.917 kb on - strand at 3298.957 kb on + strand, within BPHYT_RS14475 at 3298.957 kb on + strand, within BPHYT_RS14475 at 3298.958 kb on - strand, within BPHYT_RS14475 at 3298.958 kb on - strand, within BPHYT_RS14475 at 3298.958 kb on - strand, within BPHYT_RS14475 at 3299.207 kb on - strand, within BPHYT_RS14475 at 3299.380 kb on + strand, within BPHYT_RS14475 at 3299.380 kb on + strand, within BPHYT_RS14475 at 3299.381 kb on - strand, within BPHYT_RS14475 at 3299.513 kb on + strand at 3299.514 kb on - strand at 3299.514 kb on - strand at 3299.551 kb on + strand at 3299.552 kb on - strand at 3299.552 kb on - strand at 3299.709 kb on + strand, within BPHYT_RS14480 at 3299.710 kb on - strand, within BPHYT_RS14480 at 3299.710 kb on - strand, within BPHYT_RS14480 at 3299.825 kb on + strand, within BPHYT_RS14480 at 3299.826 kb on - strand, within BPHYT_RS14480 at 3299.826 kb on - strand, within BPHYT_RS14480 at 3299.826 kb on - strand, within BPHYT_RS14480 at 3299.826 kb on - strand, within BPHYT_RS14480
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days remove 3,297,531 - BPHYT_RS14465 0.11 -1.8 3,297,802 - BPHYT_RS14465 0.38 -1.0 3,297,802 - BPHYT_RS14465 0.38 -1.0 3,297,903 - BPHYT_RS14465 0.48 -1.2 3,297,903 - BPHYT_RS14465 0.48 +1.2 3,297,930 - BPHYT_RS14465 0.51 -0.1 3,297,969 - BPHYT_RS14465 0.55 -0.6 3,298,054 - BPHYT_RS14465 0.63 -0.2 3,298,141 - BPHYT_RS14465 0.72 -1.9 3,298,246 + BPHYT_RS14465 0.82 -0.0 3,298,247 - BPHYT_RS14465 0.82 -0.2 3,298,324 - BPHYT_RS14465 0.90 +1.0 3,298,602 - BPHYT_RS14470 0.35 -0.8 3,298,618 - BPHYT_RS14470 0.39 -0.6 3,298,618 - BPHYT_RS14470 0.39 -0.1 3,298,631 + BPHYT_RS14470 0.42 +1.0 3,298,631 + BPHYT_RS14470 0.42 -0.4 3,298,632 - BPHYT_RS14470 0.43 +1.6 3,298,684 + BPHYT_RS14470 0.56 -0.8 3,298,712 + BPHYT_RS14470 0.62 -0.2 3,298,727 + BPHYT_RS14470 0.66 -2.9 3,298,917 - -0.4 3,298,957 + BPHYT_RS14475 0.15 -1.0 3,298,957 + BPHYT_RS14475 0.15 +2.0 3,298,958 - BPHYT_RS14475 0.15 +0.8 3,298,958 - BPHYT_RS14475 0.15 +0.1 3,298,958 - BPHYT_RS14475 0.15 -1.5 3,299,207 - BPHYT_RS14475 0.53 -2.1 3,299,380 + BPHYT_RS14475 0.79 +0.3 3,299,380 + BPHYT_RS14475 0.79 -0.3 3,299,381 - BPHYT_RS14475 0.79 -0.6 3,299,513 + +0.4 3,299,514 - -0.4 3,299,514 - -0.1 3,299,551 + -1.2 3,299,552 - -0.4 3,299,552 - +0.3 3,299,709 + BPHYT_RS14480 0.19 +0.3 3,299,710 - BPHYT_RS14480 0.19 -0.3 3,299,710 - BPHYT_RS14480 0.19 +1.9 3,299,825 + BPHYT_RS14480 0.31 +0.8 3,299,826 - BPHYT_RS14480 0.31 -2.8 3,299,826 - BPHYT_RS14480 0.31 -1.1 3,299,826 - BPHYT_RS14480 0.31 -1.0 3,299,826 - BPHYT_RS14480 0.31 -1.2
Or see this region's nucleotide sequence