Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS13030

Experiment: Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS13020 and BPHYT_RS13025 are separated by 136 nucleotidesBPHYT_RS13025 and BPHYT_RS13030 are separated by 222 nucleotidesBPHYT_RS13030 and BPHYT_RS13035 are separated by 349 nucleotides BPHYT_RS13020: BPHYT_RS13020 - mercuric reductase, at 2,976,054 to 2,977,454 _RS13020 BPHYT_RS13025: BPHYT_RS13025 - hypothetical protein, at 2,977,591 to 2,977,779 _RS13025 BPHYT_RS13030: BPHYT_RS13030 - diguanylate cyclase, at 2,978,002 to 2,980,308 _RS13030 BPHYT_RS13035: BPHYT_RS13035 - hypothetical protein, at 2,980,658 to 2,981,176 _RS13035 Position (kb) 2978 2979 2980 2981Strain fitness (log2 ratio) -2 -1 0 1 2at 2977.486 kb on + strandat 2977.487 kb on - strandat 2977.769 kb on - strandat 2977.845 kb on + strandat 2977.870 kb on + strandat 2978.051 kb on + strandat 2978.052 kb on - strandat 2978.078 kb on + strandat 2978.085 kb on - strandat 2978.203 kb on - strandat 2978.203 kb on - strandat 2978.265 kb on + strand, within BPHYT_RS13030at 2978.289 kb on - strand, within BPHYT_RS13030at 2978.664 kb on - strand, within BPHYT_RS13030at 2978.832 kb on + strand, within BPHYT_RS13030at 2978.833 kb on - strand, within BPHYT_RS13030at 2978.858 kb on + strand, within BPHYT_RS13030at 2978.859 kb on - strand, within BPHYT_RS13030at 2978.945 kb on + strand, within BPHYT_RS13030at 2978.945 kb on + strand, within BPHYT_RS13030at 2978.948 kb on + strand, within BPHYT_RS13030at 2978.949 kb on - strand, within BPHYT_RS13030at 2979.276 kb on - strand, within BPHYT_RS13030at 2979.573 kb on + strandat 2979.574 kb on - strand, within BPHYT_RS13030at 2979.827 kb on + strand, within BPHYT_RS13030at 2979.827 kb on + strand, within BPHYT_RS13030at 2979.830 kb on + strand, within BPHYT_RS13030at 2979.831 kb on - strand, within BPHYT_RS13030at 2979.980 kb on + strand, within BPHYT_RS13030at 2980.157 kb on - strandat 2980.308 kb on + strandat 2980.308 kb on + strandat 2980.308 kb on + strandat 2980.308 kb on + strandat 2980.308 kb on + strandat 2980.309 kb on - strandat 2980.309 kb on - strandat 2980.309 kb on - strandat 2980.341 kb on - strandat 2980.341 kb on - strandat 2980.383 kb on + strandat 2980.384 kb on - strandat 2980.689 kb on - strandat 2980.868 kb on + strand, within BPHYT_RS13035at 2980.868 kb on + strand, within BPHYT_RS13035at 2981.288 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days
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2,977,486 + +0.0
2,977,487 - +2.0
2,977,769 - -1.1
2,977,845 + -0.6
2,977,870 + +0.6
2,978,051 + -1.0
2,978,052 - +0.0
2,978,078 + -0.0
2,978,085 - -0.4
2,978,203 - +0.9
2,978,203 - +0.1
2,978,265 + BPHYT_RS13030 0.11 +1.1
2,978,289 - BPHYT_RS13030 0.12 +2.2
2,978,664 - BPHYT_RS13030 0.29 +0.3
2,978,832 + BPHYT_RS13030 0.36 +0.3
2,978,833 - BPHYT_RS13030 0.36 -0.3
2,978,858 + BPHYT_RS13030 0.37 +0.7
2,978,859 - BPHYT_RS13030 0.37 -1.1
2,978,945 + BPHYT_RS13030 0.41 -1.4
2,978,945 + BPHYT_RS13030 0.41 -0.8
2,978,948 + BPHYT_RS13030 0.41 +1.9
2,978,949 - BPHYT_RS13030 0.41 +1.8
2,979,276 - BPHYT_RS13030 0.55 +1.0
2,979,573 + +0.0
2,979,574 - BPHYT_RS13030 0.68 +0.6
2,979,827 + BPHYT_RS13030 0.79 -2.1
2,979,827 + BPHYT_RS13030 0.79 +0.5
2,979,830 + BPHYT_RS13030 0.79 -0.5
2,979,831 - BPHYT_RS13030 0.79 +1.3
2,979,980 + BPHYT_RS13030 0.86 -0.1
2,980,157 - -0.3
2,980,308 + -0.1
2,980,308 + +0.3
2,980,308 + +0.8
2,980,308 + -1.0
2,980,308 + -0.4
2,980,309 - -0.4
2,980,309 - +1.1
2,980,309 - -0.1
2,980,341 - -0.4
2,980,341 - -0.2
2,980,383 + +2.2
2,980,384 - -0.5
2,980,689 - +1.1
2,980,868 + BPHYT_RS13035 0.40 +0.7
2,980,868 + BPHYT_RS13035 0.40 -0.4
2,981,288 - -1.0

Or see this region's nucleotide sequence