Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS11220

Experiment: Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS11215 and BPHYT_RS11220 are separated by 309 nucleotidesBPHYT_RS11220 and BPHYT_RS11225 are separated by 361 nucleotides BPHYT_RS11215: BPHYT_RS11215 - ribose ABC transporter ATPase, at 2,550,786 to 2,552,384 _RS11215 BPHYT_RS11220: BPHYT_RS11220 - LacI family transcriptional regulator, at 2,552,694 to 2,553,656 _RS11220 BPHYT_RS11225: BPHYT_RS11225 - magnesium transporter, at 2,554,018 to 2,554,995 _RS11225 Position (kb) 2552 2553 2554Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5at 2551.766 kb on - strand, within BPHYT_RS11215at 2551.766 kb on - strand, within BPHYT_RS11215at 2551.766 kb on - strand, within BPHYT_RS11215at 2551.766 kb on - strand, within BPHYT_RS11215at 2551.766 kb on - strand, within BPHYT_RS11215at 2552.231 kb on + strandat 2552.232 kb on - strandat 2552.232 kb on - strandat 2552.232 kb on - strandat 2552.321 kb on - strandat 2552.549 kb on + strandat 2552.696 kb on - strandat 2552.696 kb on - strandat 2552.696 kb on - strandat 2552.696 kb on - strandat 2553.117 kb on - strand, within BPHYT_RS11220at 2553.178 kb on + strand, within BPHYT_RS11220at 2553.476 kb on - strand, within BPHYT_RS11220at 2553.661 kb on - strandat 2553.661 kb on - strandat 2553.661 kb on - strandat 2553.661 kb on - strandat 2553.697 kb on - strandat 2553.703 kb on - strandat 2553.955 kb on - strandat 2554.100 kb on + strandat 2554.142 kb on + strand, within BPHYT_RS11225at 2554.142 kb on + strand, within BPHYT_RS11225at 2554.311 kb on - strand, within BPHYT_RS11225at 2554.325 kb on + strand, within BPHYT_RS11225at 2554.401 kb on + strand, within BPHYT_RS11225at 2554.440 kb on - strand, within BPHYT_RS11225

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days
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2,551,766 - BPHYT_RS11215 0.61 -0.4
2,551,766 - BPHYT_RS11215 0.61 +0.1
2,551,766 - BPHYT_RS11215 0.61 -0.8
2,551,766 - BPHYT_RS11215 0.61 -0.6
2,551,766 - BPHYT_RS11215 0.61 +0.3
2,552,231 + +4.9
2,552,232 - +0.8
2,552,232 - +0.4
2,552,232 - -0.1
2,552,321 - +2.9
2,552,549 + +2.6
2,552,696 - -0.6
2,552,696 - -0.4
2,552,696 - -1.1
2,552,696 - -0.1
2,553,117 - BPHYT_RS11220 0.44 -0.5
2,553,178 + BPHYT_RS11220 0.50 +0.7
2,553,476 - BPHYT_RS11220 0.81 -0.7
2,553,661 - -0.1
2,553,661 - -2.2
2,553,661 - +0.3
2,553,661 - -1.1
2,553,697 - -0.6
2,553,703 - -0.1
2,553,955 - -0.1
2,554,100 + +0.3
2,554,142 + BPHYT_RS11225 0.13 -0.9
2,554,142 + BPHYT_RS11225 0.13 +0.6
2,554,311 - BPHYT_RS11225 0.30 +1.6
2,554,325 + BPHYT_RS11225 0.31 -0.0
2,554,401 + BPHYT_RS11225 0.39 -2.2
2,554,440 - BPHYT_RS11225 0.43 -0.8

Or see this region's nucleotide sequence