Experiment: Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10950 and BPHYT_RS10955 are separated by 31 nucleotides BPHYT_RS10955 and BPHYT_RS10960 are separated by 592 nucleotides BPHYT_RS10960 and BPHYT_RS10965 are separated by 53 nucleotides
BPHYT_RS10950: BPHYT_RS10950 - transcriptional regulator, at 2,491,739 to 2,492,611
_RS10950
BPHYT_RS10955: BPHYT_RS10955 - dioxygenase, at 2,492,643 to 2,493,575
_RS10955
BPHYT_RS10960: BPHYT_RS10960 - hypothetical protein, at 2,494,168 to 2,494,281
_RS10960
BPHYT_RS10965: BPHYT_RS10965 - hypothetical protein, at 2,494,335 to 2,494,724
_RS10965
Position (kb)
2492
2493
2494 Strain fitness (log2 ratio)
-2
-1
0
1 at 2491.683 kb on + strand at 2492.150 kb on + strand, within BPHYT_RS10950 at 2492.151 kb on - strand, within BPHYT_RS10950 at 2492.221 kb on + strand, within BPHYT_RS10950 at 2492.222 kb on - strand, within BPHYT_RS10950 at 2492.628 kb on + strand at 2492.883 kb on + strand, within BPHYT_RS10955 at 2493.311 kb on - strand, within BPHYT_RS10955 at 2493.579 kb on - strand at 2493.579 kb on - strand at 2494.121 kb on - strand at 2494.133 kb on + strand at 2494.317 kb on - strand at 2494.557 kb on + strand, within BPHYT_RS10965
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days remove 2,491,683 + +0.6 2,492,150 + BPHYT_RS10950 0.47 +0.6 2,492,151 - BPHYT_RS10950 0.47 -2.7 2,492,221 + BPHYT_RS10950 0.55 +0.2 2,492,222 - BPHYT_RS10950 0.55 -0.9 2,492,628 + -0.4 2,492,883 + BPHYT_RS10955 0.26 -0.3 2,493,311 - BPHYT_RS10955 0.72 +0.3 2,493,579 - -1.6 2,493,579 - +0.4 2,494,121 - +0.6 2,494,133 + +0.1 2,494,317 - -0.2 2,494,557 + BPHYT_RS10965 0.57 -0.1
Or see this region's nucleotide sequence