Experiment: Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03240 and BPHYT_RS03245 are separated by 148 nucleotides BPHYT_RS03245 and BPHYT_RS03250 are separated by 238 nucleotides BPHYT_RS03250 and BPHYT_RS03255 are separated by 19 nucleotides BPHYT_RS03255 and BPHYT_RS03260 are separated by 160 nucleotides
BPHYT_RS03240: BPHYT_RS03240 - formate dehydrogenase accessory protein FdhD, at 728,323 to 729,162
_RS03240
BPHYT_RS03245: BPHYT_RS03245 - hypothetical protein, at 729,311 to 729,787
_RS03245
BPHYT_RS03250: BPHYT_RS03250 - diguanylate cyclase, at 730,026 to 733,136
_RS03250
BPHYT_RS03255: BPHYT_RS03255 - glutathione S-transferase, at 733,156 to 733,770
_RS03255
BPHYT_RS03260: BPHYT_RS03260 - glycine cleavage system regulatory protein, at 733,931 to 734,860
_RS03260
Position (kb) 730
731
732
733
734 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 729.165 kb on + strand at 729.166 kb on - strand at 729.166 kb on - strand at 729.166 kb on - strand at 729.249 kb on + strand at 729.258 kb on - strand at 729.258 kb on - strand at 729.306 kb on + strand at 729.306 kb on + strand at 729.306 kb on + strand at 729.307 kb on - strand at 729.307 kb on - strand at 729.307 kb on - strand at 729.307 kb on - strand at 729.307 kb on - strand at 729.374 kb on + strand, within BPHYT_RS03245 at 729.374 kb on + strand, within BPHYT_RS03245 at 729.374 kb on + strand, within BPHYT_RS03245 at 729.374 kb on + strand, within BPHYT_RS03245 at 729.375 kb on - strand, within BPHYT_RS03245 at 729.625 kb on + strand, within BPHYT_RS03245 at 729.626 kb on - strand, within BPHYT_RS03245 at 729.705 kb on - strand, within BPHYT_RS03245 at 729.786 kb on - strand at 729.786 kb on - strand at 729.789 kb on + strand at 729.789 kb on + strand at 729.789 kb on + strand at 729.789 kb on + strand at 729.820 kb on + strand at 729.820 kb on + strand at 729.821 kb on - strand at 729.824 kb on + strand at 729.859 kb on - strand at 729.896 kb on + strand at 729.943 kb on + strand at 730.107 kb on + strand at 730.107 kb on + strand at 730.108 kb on - strand at 730.109 kb on + strand at 730.110 kb on - strand at 730.110 kb on - strand at 730.186 kb on - strand at 730.458 kb on + strand, within BPHYT_RS03250 at 730.458 kb on + strand, within BPHYT_RS03250 at 730.459 kb on - strand, within BPHYT_RS03250 at 730.459 kb on - strand, within BPHYT_RS03250 at 730.464 kb on + strand, within BPHYT_RS03250 at 730.465 kb on - strand, within BPHYT_RS03250 at 730.740 kb on + strand, within BPHYT_RS03250 at 730.740 kb on + strand, within BPHYT_RS03250 at 730.740 kb on + strand, within BPHYT_RS03250 at 730.741 kb on - strand, within BPHYT_RS03250 at 730.741 kb on - strand, within BPHYT_RS03250 at 730.741 kb on - strand, within BPHYT_RS03250 at 730.741 kb on - strand, within BPHYT_RS03250 at 730.741 kb on - strand, within BPHYT_RS03250 at 730.742 kb on + strand, within BPHYT_RS03250 at 730.800 kb on + strand, within BPHYT_RS03250 at 730.801 kb on - strand, within BPHYT_RS03250 at 730.929 kb on - strand, within BPHYT_RS03250 at 731.406 kb on + strand, within BPHYT_RS03250 at 731.418 kb on - strand, within BPHYT_RS03250 at 731.555 kb on + strand, within BPHYT_RS03250 at 731.691 kb on + strand, within BPHYT_RS03250 at 731.692 kb on - strand, within BPHYT_RS03250 at 731.692 kb on - strand, within BPHYT_RS03250 at 731.692 kb on - strand, within BPHYT_RS03250 at 731.838 kb on + strand, within BPHYT_RS03250 at 731.838 kb on + strand, within BPHYT_RS03250 at 731.839 kb on - strand, within BPHYT_RS03250 at 732.189 kb on + strand, within BPHYT_RS03250 at 732.190 kb on - strand, within BPHYT_RS03250 at 732.276 kb on + strand, within BPHYT_RS03250 at 732.409 kb on - strand, within BPHYT_RS03250 at 732.409 kb on - strand, within BPHYT_RS03250 at 732.715 kb on - strand, within BPHYT_RS03250 at 732.834 kb on + strand at 732.849 kb on + strand at 732.850 kb on - strand at 733.056 kb on + strand at 733.057 kb on - strand at 733.057 kb on - strand at 733.057 kb on - strand at 733.111 kb on + strand at 733.112 kb on - strand at 733.566 kb on - strand, within BPHYT_RS03255 at 733.661 kb on + strand, within BPHYT_RS03255 at 733.662 kb on - strand, within BPHYT_RS03255 at 733.709 kb on - strand at 733.791 kb on + strand at 733.792 kb on - strand at 733.869 kb on + strand at 733.869 kb on + strand at 733.902 kb on + strand at 733.903 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days remove 729,165 + -1.6 729,166 - +0.9 729,166 - +0.1 729,166 - +0.1 729,249 + -1.0 729,258 - +0.3 729,258 - +0.8 729,306 + +0.8 729,306 + -0.3 729,306 + -0.1 729,307 - +0.7 729,307 - -1.0 729,307 - -0.2 729,307 - +0.1 729,307 - +0.0 729,374 + BPHYT_RS03245 0.13 +2.7 729,374 + BPHYT_RS03245 0.13 -0.5 729,374 + BPHYT_RS03245 0.13 -2.1 729,374 + BPHYT_RS03245 0.13 +1.2 729,375 - BPHYT_RS03245 0.13 -0.9 729,625 + BPHYT_RS03245 0.66 -1.2 729,626 - BPHYT_RS03245 0.66 +1.3 729,705 - BPHYT_RS03245 0.83 -0.4 729,786 - -0.3 729,786 - +0.1 729,789 + +0.9 729,789 + -0.4 729,789 + -0.2 729,789 + -1.9 729,820 + -0.5 729,820 + -0.8 729,821 - +0.0 729,824 + +0.6 729,859 - +0.5 729,896 + -0.0 729,943 + -0.8 730,107 + -0.3 730,107 + +0.2 730,108 - -0.2 730,109 + +1.1 730,110 - -0.1 730,110 - -1.1 730,186 - +0.5 730,458 + BPHYT_RS03250 0.14 +0.2 730,458 + BPHYT_RS03250 0.14 -0.9 730,459 - BPHYT_RS03250 0.14 -2.5 730,459 - BPHYT_RS03250 0.14 -0.3 730,464 + BPHYT_RS03250 0.14 -0.7 730,465 - BPHYT_RS03250 0.14 -2.9 730,740 + BPHYT_RS03250 0.23 +0.6 730,740 + BPHYT_RS03250 0.23 -0.5 730,740 + BPHYT_RS03250 0.23 -0.2 730,741 - BPHYT_RS03250 0.23 -0.1 730,741 - BPHYT_RS03250 0.23 +0.6 730,741 - BPHYT_RS03250 0.23 +0.6 730,741 - BPHYT_RS03250 0.23 -1.0 730,741 - BPHYT_RS03250 0.23 -1.7 730,742 + BPHYT_RS03250 0.23 -0.2 730,800 + BPHYT_RS03250 0.25 +0.6 730,801 - BPHYT_RS03250 0.25 -0.4 730,929 - BPHYT_RS03250 0.29 +1.2 731,406 + BPHYT_RS03250 0.44 -0.5 731,418 - BPHYT_RS03250 0.45 -2.9 731,555 + BPHYT_RS03250 0.49 -0.4 731,691 + BPHYT_RS03250 0.54 -4.3 731,692 - BPHYT_RS03250 0.54 +1.4 731,692 - BPHYT_RS03250 0.54 -1.5 731,692 - BPHYT_RS03250 0.54 -0.8 731,838 + BPHYT_RS03250 0.58 -1.5 731,838 + BPHYT_RS03250 0.58 +0.0 731,839 - BPHYT_RS03250 0.58 -0.6 732,189 + BPHYT_RS03250 0.70 -0.3 732,190 - BPHYT_RS03250 0.70 -0.4 732,276 + BPHYT_RS03250 0.72 -0.2 732,409 - BPHYT_RS03250 0.77 -0.3 732,409 - BPHYT_RS03250 0.77 +0.1 732,715 - BPHYT_RS03250 0.86 -0.3 732,834 + -0.1 732,849 + -3.5 732,850 - +0.5 733,056 + -0.6 733,057 - +0.7 733,057 - -1.0 733,057 - +0.1 733,111 + -1.8 733,112 - -0.2 733,566 - BPHYT_RS03255 0.67 -1.1 733,661 + BPHYT_RS03255 0.82 -0.5 733,662 - BPHYT_RS03255 0.82 -0.5 733,709 - -1.9 733,791 + +3.7 733,792 - -1.8 733,869 + +1.5 733,869 + -1.0 733,902 + -0.2 733,903 - -0.5
Or see this region's nucleotide sequence