Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS02220

Experiment: Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS02215 and BPHYT_RS02220 are separated by 7 nucleotidesBPHYT_RS02220 and BPHYT_RS02225 are separated by 27 nucleotidesBPHYT_RS02225 and BPHYT_RS02230 are separated by 47 nucleotides BPHYT_RS02215: BPHYT_RS02215 - hypothetical protein, at 509,761 to 510,210 _RS02215 BPHYT_RS02220: BPHYT_RS02220 - butyryl-CoA dehydrogenase, at 510,218 to 511,348 _RS02220 BPHYT_RS02225: BPHYT_RS02225 - short-chain dehydrogenase, at 511,376 to 512,053 _RS02225 BPHYT_RS02230: BPHYT_RS02230 - acetyl-CoA acetyltransferase, at 512,101 to 513,294 _RS02230 Position (kb) 510 511 512Strain fitness (log2 ratio) -2 -1 0 1at 509.508 kb on + strandat 509.705 kb on + strandat 509.706 kb on + strandat 509.706 kb on + strandat 509.707 kb on - strandat 509.707 kb on - strandat 510.271 kb on - strandat 510.271 kb on - strandat 510.913 kb on - strand, within BPHYT_RS02220at 510.913 kb on - strand, within BPHYT_RS02220at 511.402 kb on - strandat 511.407 kb on + strandat 511.993 kb on - strandat 511.993 kb on - strandat 512.056 kb on - strandat 512.056 kb on - strandat 512.056 kb on - strandat 512.058 kb on + strandat 512.058 kb on + strandat 512.342 kb on + strand, within BPHYT_RS02230

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days
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509,508 + -2.2
509,705 + +0.8
509,706 + -1.0
509,706 + -0.5
509,707 - -1.1
509,707 - +0.3
510,271 - +1.0
510,271 - -0.3
510,913 - BPHYT_RS02220 0.61 -0.6
510,913 - BPHYT_RS02220 0.61 -0.8
511,402 - -0.7
511,407 + +0.8
511,993 - +1.2
511,993 - +0.9
512,056 - -1.0
512,056 - -0.2
512,056 - -0.9
512,058 + -0.7
512,058 + -0.3
512,342 + BPHYT_RS02230 0.20 -1.5

Or see this region's nucleotide sequence