Experiment: Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02215 and BPHYT_RS02220 are separated by 7 nucleotides BPHYT_RS02220 and BPHYT_RS02225 are separated by 27 nucleotides BPHYT_RS02225 and BPHYT_RS02230 are separated by 47 nucleotides
BPHYT_RS02215: BPHYT_RS02215 - hypothetical protein, at 509,761 to 510,210
_RS02215
BPHYT_RS02220: BPHYT_RS02220 - butyryl-CoA dehydrogenase, at 510,218 to 511,348
_RS02220
BPHYT_RS02225: BPHYT_RS02225 - short-chain dehydrogenase, at 511,376 to 512,053
_RS02225
BPHYT_RS02230: BPHYT_RS02230 - acetyl-CoA acetyltransferase, at 512,101 to 513,294
_RS02230
Position (kb)
510
511
512 Strain fitness (log2 ratio)
-2
-1
0
1 at 509.508 kb on + strand at 509.705 kb on + strand at 509.706 kb on + strand at 509.706 kb on + strand at 509.707 kb on - strand at 509.707 kb on - strand at 510.271 kb on - strand at 510.271 kb on - strand at 510.913 kb on - strand, within BPHYT_RS02220 at 510.913 kb on - strand, within BPHYT_RS02220 at 511.402 kb on - strand at 511.407 kb on + strand at 511.993 kb on - strand at 511.993 kb on - strand at 512.056 kb on - strand at 512.056 kb on - strand at 512.056 kb on - strand at 512.058 kb on + strand at 512.058 kb on + strand at 512.342 kb on + strand, within BPHYT_RS02230
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days remove 509,508 + -2.2 509,705 + +0.8 509,706 + -1.0 509,706 + -0.5 509,707 - -1.1 509,707 - +0.3 510,271 - +1.0 510,271 - -0.3 510,913 - BPHYT_RS02220 0.61 -0.6 510,913 - BPHYT_RS02220 0.61 -0.8 511,402 - -0.7 511,407 + +0.8 511,993 - +1.2 511,993 - +0.9 512,056 - -1.0 512,056 - -0.2 512,056 - -0.9 512,058 + -0.7 512,058 + -0.3 512,342 + BPHYT_RS02230 0.20 -1.5
Or see this region's nucleotide sequence