Experiment: Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00510 and BPHYT_RS00515 are separated by 54 nucleotides BPHYT_RS00515 and BPHYT_RS00520 are separated by 193 nucleotides BPHYT_RS00520 and BPHYT_RS00525 are separated by 369 nucleotides
BPHYT_RS00510: BPHYT_RS00510 - S-(hydroxymethyl)glutathione dehydrogenase, at 123,622 to 124,731
_RS00510
BPHYT_RS00515: BPHYT_RS00515 - regulator, at 124,786 to 125,061
_RS00515
BPHYT_RS00520: BPHYT_RS00520 - glutamate--cysteine ligase, at 125,255 to 126,853
_RS00520
BPHYT_RS00525: BPHYT_RS00525 - aldehyde dismutase, at 127,223 to 128,437
_RS00525
Position (kb) 125
126
127 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 124.295 kb on - strand, within BPHYT_RS00510 at 124.328 kb on + strand, within BPHYT_RS00510 at 124.329 kb on - strand, within BPHYT_RS00510 at 124.352 kb on + strand, within BPHYT_RS00510 at 124.369 kb on - strand, within BPHYT_RS00510 at 124.608 kb on + strand, within BPHYT_RS00510 at 124.747 kb on - strand at 125.054 kb on + strand at 125.082 kb on - strand at 125.095 kb on + strand at 125.218 kb on - strand at 125.256 kb on + strand at 125.256 kb on + strand at 125.398 kb on + strand at 125.955 kb on + strand, within BPHYT_RS00520 at 126.034 kb on - strand, within BPHYT_RS00520 at 126.036 kb on - strand, within BPHYT_RS00520 at 126.078 kb on + strand, within BPHYT_RS00520 at 126.096 kb on + strand, within BPHYT_RS00520 at 126.108 kb on - strand, within BPHYT_RS00520 at 126.174 kb on + strand, within BPHYT_RS00520 at 126.174 kb on + strand, within BPHYT_RS00520 at 126.279 kb on + strand, within BPHYT_RS00520 at 126.280 kb on - strand, within BPHYT_RS00520 at 126.307 kb on + strand, within BPHYT_RS00520 at 126.307 kb on + strand, within BPHYT_RS00520 at 126.308 kb on - strand, within BPHYT_RS00520 at 126.309 kb on + strand, within BPHYT_RS00520 at 126.310 kb on - strand, within BPHYT_RS00520 at 126.310 kb on - strand, within BPHYT_RS00520 at 126.399 kb on + strand, within BPHYT_RS00520 at 126.399 kb on + strand, within BPHYT_RS00520 at 126.399 kb on + strand, within BPHYT_RS00520 at 126.427 kb on + strand, within BPHYT_RS00520 at 126.495 kb on + strand, within BPHYT_RS00520 at 126.495 kb on + strand at 126.495 kb on + strand, within BPHYT_RS00520 at 126.496 kb on - strand, within BPHYT_RS00520 at 126.496 kb on - strand, within BPHYT_RS00520 at 126.496 kb on - strand, within BPHYT_RS00520 at 126.848 kb on - strand at 126.849 kb on + strand at 126.850 kb on - strand at 126.850 kb on - strand at 126.883 kb on - strand at 126.924 kb on + strand at 126.996 kb on - strand at 127.022 kb on - strand at 127.101 kb on - strand at 127.185 kb on + strand at 127.217 kb on - strand at 127.257 kb on + strand at 127.257 kb on + strand at 127.290 kb on + strand at 127.290 kb on + strand at 127.348 kb on - strand, within BPHYT_RS00525 at 127.512 kb on + strand, within BPHYT_RS00525 at 127.552 kb on - strand, within BPHYT_RS00525 at 127.632 kb on + strand, within BPHYT_RS00525 at 127.632 kb on + strand, within BPHYT_RS00525
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days remove 124,295 - BPHYT_RS00510 0.61 +0.8 124,328 + BPHYT_RS00510 0.64 -0.5 124,329 - BPHYT_RS00510 0.64 +0.7 124,352 + BPHYT_RS00510 0.66 +1.3 124,369 - BPHYT_RS00510 0.67 -0.6 124,608 + BPHYT_RS00510 0.89 -1.5 124,747 - -0.1 125,054 + -0.5 125,082 - +0.7 125,095 + +2.4 125,218 - -1.1 125,256 + -0.7 125,256 + +0.5 125,398 + -0.7 125,955 + BPHYT_RS00520 0.44 -1.8 126,034 - BPHYT_RS00520 0.49 +0.2 126,036 - BPHYT_RS00520 0.49 -0.7 126,078 + BPHYT_RS00520 0.51 -1.4 126,096 + BPHYT_RS00520 0.53 -2.5 126,108 - BPHYT_RS00520 0.53 +0.0 126,174 + BPHYT_RS00520 0.57 -0.1 126,174 + BPHYT_RS00520 0.57 -0.6 126,279 + BPHYT_RS00520 0.64 -1.4 126,280 - BPHYT_RS00520 0.64 +0.5 126,307 + BPHYT_RS00520 0.66 -0.6 126,307 + BPHYT_RS00520 0.66 +1.6 126,308 - BPHYT_RS00520 0.66 -2.1 126,309 + BPHYT_RS00520 0.66 -0.1 126,310 - BPHYT_RS00520 0.66 +1.9 126,310 - BPHYT_RS00520 0.66 -1.0 126,399 + BPHYT_RS00520 0.72 -1.0 126,399 + BPHYT_RS00520 0.72 -0.5 126,399 + BPHYT_RS00520 0.72 -1.2 126,427 + BPHYT_RS00520 0.73 +1.3 126,495 + BPHYT_RS00520 0.78 +1.0 126,495 + -0.8 126,495 + BPHYT_RS00520 0.78 +0.9 126,496 - BPHYT_RS00520 0.78 -0.2 126,496 - BPHYT_RS00520 0.78 -1.2 126,496 - BPHYT_RS00520 0.78 -1.9 126,848 - +0.1 126,849 + -0.6 126,850 - -0.7 126,850 - -2.6 126,883 - -0.4 126,924 + -2.8 126,996 - -0.6 127,022 - +0.8 127,101 - +0.4 127,185 + -1.1 127,217 - -0.1 127,257 + -0.2 127,257 + -1.7 127,290 + +0.3 127,290 + -0.4 127,348 - BPHYT_RS00525 0.10 -1.6 127,512 + BPHYT_RS00525 0.24 -1.0 127,552 - BPHYT_RS00525 0.27 -0.2 127,632 + BPHYT_RS00525 0.34 +0.2 127,632 + BPHYT_RS00525 0.34 -0.7
Or see this region's nucleotide sequence