Experiment: R2A control for volatile experiments
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa2217 and SMa2219 are separated by 2 nucleotides SMa2219 and SMa2221 overlap by 4 nucleotides SMa2221 and SMa2223 overlap by 4 nucleotides
SMa2217: SMa2217 - decarboxylase, at 1,241,610 to 1,242,935
SMa2217
SMa2219: SMa2219 - decarboxylase, at 1,242,938 to 1,243,531
SMa2219
SMa2221: SMa2221 - hypothetical protein, at 1,243,528 to 1,244,241
SMa2221
SMa2223: SMa2223 - oxidoreductase, at 1,244,238 to 1,245,344
SMa2223
Position (kb)
1243
1244
1245 Strain fitness (log2 ratio)
-2
-1
0
1 at 1242.681 kb on - strand, within SMa2217 at 1242.709 kb on - strand, within SMa2217 at 1242.765 kb on - strand, within SMa2217 at 1243.105 kb on - strand, within SMa2219 at 1243.342 kb on + strand, within SMa2219 at 1243.343 kb on - strand, within SMa2219 at 1243.343 kb on - strand, within SMa2219 at 1243.397 kb on + strand, within SMa2219 at 1243.397 kb on + strand, within SMa2219 at 1243.397 kb on + strand, within SMa2219 at 1243.543 kb on - strand at 1243.756 kb on - strand, within SMa2221 at 1243.871 kb on + strand, within SMa2221 at 1243.871 kb on + strand, within SMa2221 at 1243.871 kb on + strand, within SMa2221 at 1243.871 kb on + strand, within SMa2221 at 1243.871 kb on + strand, within SMa2221 at 1243.872 kb on - strand, within SMa2221 at 1243.872 kb on - strand, within SMa2221 at 1243.872 kb on - strand, within SMa2221 at 1243.872 kb on - strand, within SMa2221 at 1243.872 kb on - strand, within SMa2221 at 1243.872 kb on - strand, within SMa2221 at 1243.872 kb on - strand, within SMa2221 at 1243.872 kb on - strand, within SMa2221 at 1243.872 kb on - strand, within SMa2221 at 1244.058 kb on - strand, within SMa2221 at 1244.138 kb on - strand, within SMa2221 at 1244.195 kb on + strand at 1244.211 kb on - strand at 1244.327 kb on + strand at 1244.421 kb on + strand, within SMa2223 at 1244.421 kb on + strand, within SMa2223 at 1244.421 kb on + strand, within SMa2223 at 1244.501 kb on - strand, within SMa2223 at 1244.570 kb on + strand, within SMa2223 at 1244.645 kb on - strand, within SMa2223 at 1244.654 kb on - strand, within SMa2223 at 1244.767 kb on + strand, within SMa2223 at 1245.182 kb on + strand, within SMa2223 at 1245.182 kb on + strand, within SMa2223 at 1245.186 kb on + strand, within SMa2223 at 1245.186 kb on + strand, within SMa2223 at 1245.186 kb on + strand, within SMa2223 at 1245.187 kb on - strand, within SMa2223 at 1245.187 kb on - strand, within SMa2223 at 1245.216 kb on + strand, within SMa2223
Per-strain Table
Position Strand Gene LocusTag Fraction R2A control for volatile experiments remove 1,242,681 - SMa2217 0.81 +0.6 1,242,709 - SMa2217 0.83 -0.1 1,242,765 - SMa2217 0.87 +0.6 1,243,105 - SMa2219 0.28 -1.8 1,243,342 + SMa2219 0.68 -0.6 1,243,343 - SMa2219 0.68 -1.1 1,243,343 - SMa2219 0.68 +0.3 1,243,397 + SMa2219 0.77 +0.9 1,243,397 + SMa2219 0.77 -0.6 1,243,397 + SMa2219 0.77 -0.3 1,243,543 - +0.1 1,243,756 - SMa2221 0.32 +0.2 1,243,871 + SMa2221 0.48 -0.7 1,243,871 + SMa2221 0.48 +0.4 1,243,871 + SMa2221 0.48 -0.0 1,243,871 + SMa2221 0.48 -1.2 1,243,871 + SMa2221 0.48 -0.1 1,243,872 - SMa2221 0.48 +0.2 1,243,872 - SMa2221 0.48 +0.3 1,243,872 - SMa2221 0.48 +0.4 1,243,872 - SMa2221 0.48 +0.4 1,243,872 - SMa2221 0.48 +0.7 1,243,872 - SMa2221 0.48 +0.5 1,243,872 - SMa2221 0.48 +0.5 1,243,872 - SMa2221 0.48 +1.0 1,243,872 - SMa2221 0.48 +1.3 1,244,058 - SMa2221 0.74 +0.9 1,244,138 - SMa2221 0.85 +1.4 1,244,195 + +0.2 1,244,211 - -0.7 1,244,327 + -0.2 1,244,421 + SMa2223 0.17 +0.6 1,244,421 + SMa2223 0.17 -0.2 1,244,421 + SMa2223 0.17 +0.7 1,244,501 - SMa2223 0.24 -1.5 1,244,570 + SMa2223 0.30 -0.6 1,244,645 - SMa2223 0.37 +0.4 1,244,654 - SMa2223 0.38 +0.8 1,244,767 + SMa2223 0.48 -2.7 1,245,182 + SMa2223 0.85 +0.1 1,245,182 + SMa2223 0.85 -1.7 1,245,186 + SMa2223 0.86 -0.2 1,245,186 + SMa2223 0.86 +0.4 1,245,186 + SMa2223 0.86 +0.4 1,245,187 - SMa2223 0.86 -0.2 1,245,187 - SMa2223 0.86 -2.1 1,245,216 + SMa2223 0.88 +0.5
Or see this region's nucleotide sequence