Experiment: R2A control for volatile experiments
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1318 and SMa1319 are separated by 4 nucleotides SMa1319 and SMa1321 overlap by 4 nucleotides SMa1321 and SMa1322 are separated by 3 nucleotides SMa1322 and SMa1323 are separated by 96 nucleotides SMa1323 and SMa1325 are separated by 260 nucleotides
SMa1318: SMa1318 - VirB3 type IV secretion protein, at 719,504 to 719,845
SMa1318
SMa1319: SMa1319 - VirB2 type IV secretion protein, at 719,850 to 720,149
SMa1319
SMa1321: SMa1321 - VirB1 type IV secretion protein, at 720,146 to 720,817
SMa1321
SMa1322: SMa1322 - hypothetical protein, at 720,821 to 721,339
SMa1322
SMa1323: SMa1323 - Transcription regulator, at 721,436 to 721,804
SMa1323
SMa1325: SMa1325 - hypothetical protein, at 722,065 to 722,316
SMa1325
Position (kb)
720
721
722 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 719.941 kb on + strand, within SMa1319 at 720.005 kb on + strand, within SMa1319 at 720.429 kb on + strand, within SMa1321 at 720.429 kb on + strand, within SMa1321 at 720.430 kb on - strand, within SMa1321 at 720.432 kb on + strand, within SMa1321 at 720.432 kb on + strand, within SMa1321 at 720.499 kb on - strand, within SMa1321 at 720.499 kb on - strand, within SMa1321 at 720.773 kb on + strand at 720.773 kb on + strand at 720.774 kb on - strand at 720.774 kb on - strand at 720.775 kb on + strand at 720.775 kb on + strand at 720.870 kb on + strand at 720.871 kb on - strand at 720.910 kb on - strand, within SMa1322 at 720.910 kb on - strand, within SMa1322 at 721.024 kb on + strand, within SMa1322 at 721.024 kb on + strand, within SMa1322 at 721.080 kb on + strand, within SMa1322 at 721.081 kb on - strand, within SMa1322 at 721.081 kb on - strand, within SMa1322 at 721.083 kb on + strand, within SMa1322 at 721.093 kb on - strand, within SMa1322 at 721.234 kb on + strand, within SMa1322 at 721.268 kb on - strand, within SMa1322 at 721.284 kb on + strand, within SMa1322 at 721.285 kb on - strand, within SMa1322 at 721.295 kb on + strand at 721.296 kb on - strand at 721.474 kb on - strand, within SMa1323 at 721.572 kb on - strand, within SMa1323 at 721.687 kb on + strand, within SMa1323 at 721.687 kb on + strand, within SMa1323 at 721.768 kb on - strand at 721.823 kb on - strand at 721.918 kb on + strand at 721.918 kb on + strand at 721.918 kb on + strand at 721.919 kb on - strand at 721.922 kb on - strand at 722.206 kb on + strand, within SMa1325 at 722.253 kb on - strand, within SMa1325 at 722.299 kb on + strand at 722.300 kb on - strand at 722.300 kb on - strand at 722.300 kb on - strand at 722.300 kb on - strand at 722.300 kb on - strand at 722.324 kb on + strand at 722.325 kb on - strand at 722.335 kb on + strand at 722.336 kb on - strand at 722.336 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A control for volatile experiments remove 719,941 + SMa1319 0.30 -0.5 720,005 + SMa1319 0.52 -0.3 720,429 + SMa1321 0.42 -0.9 720,429 + SMa1321 0.42 +1.0 720,430 - SMa1321 0.42 +0.3 720,432 + SMa1321 0.43 +0.2 720,432 + SMa1321 0.43 -0.1 720,499 - SMa1321 0.53 +0.0 720,499 - SMa1321 0.53 -1.1 720,773 + +0.0 720,773 + -0.8 720,774 - -1.8 720,774 - -0.7 720,775 + -0.9 720,775 + +0.1 720,870 + -0.3 720,871 - -0.2 720,910 - SMa1322 0.17 -0.8 720,910 - SMa1322 0.17 -0.3 721,024 + SMa1322 0.39 -1.1 721,024 + SMa1322 0.39 +1.5 721,080 + SMa1322 0.50 +0.2 721,081 - SMa1322 0.50 -0.9 721,081 - SMa1322 0.50 -0.8 721,083 + SMa1322 0.50 -0.2 721,093 - SMa1322 0.52 -0.1 721,234 + SMa1322 0.80 -0.4 721,268 - SMa1322 0.86 -0.4 721,284 + SMa1322 0.89 -0.1 721,285 - SMa1322 0.89 -0.9 721,295 + +0.1 721,296 - -0.1 721,474 - SMa1323 0.10 -0.9 721,572 - SMa1323 0.37 -0.7 721,687 + SMa1323 0.68 +0.4 721,687 + SMa1323 0.68 -1.5 721,768 - -2.5 721,823 - +0.1 721,918 + -0.9 721,918 + +0.2 721,918 + -1.3 721,919 - -0.4 721,922 - +0.0 722,206 + SMa1325 0.56 -0.3 722,253 - SMa1325 0.75 +0.0 722,299 + +0.2 722,300 - -0.2 722,300 - +2.6 722,300 - +0.5 722,300 - -0.2 722,300 - +1.0 722,324 + +1.2 722,325 - -0.3 722,335 + +1.8 722,336 - -0.4 722,336 - +0.7
Or see this region's nucleotide sequence