Experiment: R2A control for volatile experiments
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20215 and SM_b20216 are separated by 140 nucleotides SM_b20216 and SM_b20217 are separated by 217 nucleotides
SM_b20215: SM_b20215 - transcriptional regulator, at 222,993 to 223,973
_b20215
SM_b20216: SM_b20216 - epoxide hydrolase, at 224,114 to 225,160
_b20216
SM_b20217: SM_b20217 - hypothetical protein, at 225,378 to 226,043
_b20217
Position (kb)
224
225
226 Strain fitness (log2 ratio)
-2
-1
0
1 at 223.129 kb on - strand, within SM_b20215 at 223.135 kb on + strand, within SM_b20215 at 223.136 kb on - strand, within SM_b20215 at 223.193 kb on + strand, within SM_b20215 at 223.194 kb on - strand, within SM_b20215 at 223.218 kb on + strand, within SM_b20215 at 223.430 kb on + strand, within SM_b20215 at 223.534 kb on - strand, within SM_b20215 at 223.666 kb on - strand, within SM_b20215 at 223.994 kb on + strand at 223.999 kb on + strand at 223.999 kb on + strand at 224.000 kb on - strand at 224.023 kb on + strand at 224.066 kb on + strand at 224.066 kb on + strand at 224.067 kb on - strand at 224.260 kb on + strand, within SM_b20216 at 224.260 kb on + strand, within SM_b20216 at 224.277 kb on + strand, within SM_b20216 at 224.307 kb on + strand, within SM_b20216 at 224.307 kb on + strand, within SM_b20216 at 224.307 kb on + strand, within SM_b20216 at 224.307 kb on + strand, within SM_b20216 at 224.308 kb on - strand, within SM_b20216 at 224.384 kb on + strand, within SM_b20216 at 224.385 kb on - strand, within SM_b20216 at 224.596 kb on + strand, within SM_b20216 at 224.651 kb on + strand, within SM_b20216 at 224.757 kb on - strand, within SM_b20216 at 224.933 kb on + strand, within SM_b20216 at 225.259 kb on + strand at 225.259 kb on + strand at 225.277 kb on + strand at 225.277 kb on + strand at 225.277 kb on + strand at 225.277 kb on + strand at 225.277 kb on + strand at 225.278 kb on - strand at 225.419 kb on + strand at 225.419 kb on + strand at 225.435 kb on - strand at 225.545 kb on + strand, within SM_b20217 at 225.624 kb on + strand, within SM_b20217 at 225.694 kb on + strand, within SM_b20217 at 225.735 kb on + strand, within SM_b20217 at 225.736 kb on - strand, within SM_b20217 at 225.787 kb on - strand, within SM_b20217 at 225.796 kb on - strand, within SM_b20217 at 225.796 kb on - strand, within SM_b20217 at 226.002 kb on + strand at 226.142 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A control for volatile experiments remove 223,129 - SM_b20215 0.14 +0.1 223,135 + SM_b20215 0.14 -0.7 223,136 - SM_b20215 0.15 -0.2 223,193 + SM_b20215 0.20 -0.1 223,194 - SM_b20215 0.20 +0.1 223,218 + SM_b20215 0.23 -1.2 223,430 + SM_b20215 0.45 -0.9 223,534 - SM_b20215 0.55 +0.7 223,666 - SM_b20215 0.69 +0.3 223,994 + -0.4 223,999 + +0.9 223,999 + +0.1 224,000 - -0.8 224,023 + +0.0 224,066 + +0.3 224,066 + +1.2 224,067 - +0.4 224,260 + SM_b20216 0.14 -0.4 224,260 + SM_b20216 0.14 -0.3 224,277 + SM_b20216 0.16 -0.3 224,307 + SM_b20216 0.18 -0.6 224,307 + SM_b20216 0.18 -1.9 224,307 + SM_b20216 0.18 -0.8 224,307 + SM_b20216 0.18 -0.4 224,308 - SM_b20216 0.19 -0.7 224,384 + SM_b20216 0.26 +0.7 224,385 - SM_b20216 0.26 -0.8 224,596 + SM_b20216 0.46 -0.3 224,651 + SM_b20216 0.51 +0.7 224,757 - SM_b20216 0.61 -0.5 224,933 + SM_b20216 0.78 +0.1 225,259 + -0.2 225,259 + +0.2 225,277 + +1.1 225,277 + +0.5 225,277 + +0.7 225,277 + -0.7 225,277 + -0.1 225,278 - -0.1 225,419 + +0.3 225,419 + +0.2 225,435 - -0.4 225,545 + SM_b20217 0.25 +0.7 225,624 + SM_b20217 0.37 +0.9 225,694 + SM_b20217 0.47 +1.1 225,735 + SM_b20217 0.54 -0.7 225,736 - SM_b20217 0.54 -0.4 225,787 - SM_b20217 0.61 -0.3 225,796 - SM_b20217 0.63 -0.2 225,796 - SM_b20217 0.63 +1.4 226,002 + +0.3 226,142 - -0.0
Or see this region's nucleotide sequence