Experiment: R2A control for volatile experiments
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc02914 and SMc00320 are separated by 159 nucleotides SMc00320 and SMc00321 are separated by 12 nucleotides
SMc02914: SMc02914 - translation initiation factor IF-2, at 270,236 to 272,905
SMc02914
SMc00320: SMc00320 - ribosome-binding factor A, at 273,065 to 273,472
SMc00320
SMc00321: SMc00321 - tRNA pseudouridine synthase B, at 273,485 to 274,420
SMc00321
Position (kb) 273
274 Strain fitness (log2 ratio) -1
0
1
2
3 at 273.162 kb on + strand, within SMc00320 at 273.163 kb on - strand, within SMc00320 at 273.163 kb on - strand, within SMc00320 at 273.242 kb on + strand, within SMc00320 at 273.243 kb on - strand, within SMc00320 at 273.243 kb on - strand, within SMc00320 at 273.350 kb on + strand, within SMc00320 at 273.395 kb on + strand, within SMc00320 at 273.395 kb on + strand, within SMc00320 at 273.396 kb on - strand, within SMc00320 at 273.470 kb on + strand at 273.470 kb on + strand at 273.690 kb on - strand, within SMc00321 at 273.896 kb on + strand, within SMc00321 at 273.897 kb on - strand, within SMc00321 at 274.022 kb on + strand, within SMc00321 at 274.022 kb on + strand, within SMc00321 at 274.022 kb on + strand, within SMc00321 at 274.022 kb on + strand, within SMc00321 at 274.022 kb on + strand, within SMc00321 at 274.022 kb on + strand, within SMc00321 at 274.022 kb on + strand, within SMc00321 at 274.023 kb on - strand, within SMc00321 at 274.023 kb on - strand, within SMc00321 at 274.334 kb on + strand at 274.418 kb on + strand at 274.419 kb on - strand at 274.452 kb on + strand at 274.453 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A control for volatile experiments remove 273,162 + SMc00320 0.24 +0.1 273,163 - SMc00320 0.24 +1.6 273,163 - SMc00320 0.24 +1.7 273,242 + SMc00320 0.43 +0.2 273,243 - SMc00320 0.44 -0.5 273,243 - SMc00320 0.44 +3.1 273,350 + SMc00320 0.70 +3.1 273,395 + SMc00320 0.81 +1.6 273,395 + SMc00320 0.81 +1.2 273,396 - SMc00320 0.81 +0.9 273,470 + -0.1 273,470 + -0.2 273,690 - SMc00321 0.22 -0.4 273,896 + SMc00321 0.44 +1.7 273,897 - SMc00321 0.44 -1.3 274,022 + SMc00321 0.57 -0.1 274,022 + SMc00321 0.57 -0.5 274,022 + SMc00321 0.57 +0.3 274,022 + SMc00321 0.57 +0.5 274,022 + SMc00321 0.57 +0.3 274,022 + SMc00321 0.57 +0.3 274,022 + SMc00321 0.57 -1.1 274,023 - SMc00321 0.57 +1.4 274,023 - SMc00321 0.57 -1.3 274,334 + -0.4 274,418 + -0.5 274,419 - -0.3 274,452 + -1.2 274,453 - -0.6
Or see this region's nucleotide sequence