Experiment: R2A control for volatile experiments
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0329 and SMa5017 are separated by 55 nucleotides SMa5017 and SMa0333 overlap by 4 nucleotides SMa0333 and SMa0335 are separated by 180 nucleotides SMa0335 and SMa0337 overlap by 14 nucleotides
SMa0329: SMa0329 - short chain alcohol dehydrogenase-related dehydrogenase, at 182,145 to 182,906
SMa0329
SMa5017: SMa5017 - hypothetical protein, at 182,962 to 183,186
SMa5017
SMa0333: SMa0333 - hypothetical protein, at 183,183 to 183,395
SMa0333
SMa0335: SMa0335 - 3-ketoacyl-ACP reductase, at 183,576 to 184,352
SMa0335
SMa0337: SMa0337 - hypothetical protein, at 184,339 to 184,821
SMa0337
Position (kb) 183
184 Strain fitness (log2 ratio) -2
-1
0
1 at 182.499 kb on + strand, within SMa0329 at 182.500 kb on - strand, within SMa0329 at 182.500 kb on - strand, within SMa0329 at 182.682 kb on - strand, within SMa0329 at 182.700 kb on - strand, within SMa0329 at 183.231 kb on - strand, within SMa0333 at 183.238 kb on + strand, within SMa0333 at 183.238 kb on + strand, within SMa0333 at 183.381 kb on - strand at 183.381 kb on - strand at 183.628 kb on + strand at 183.628 kb on + strand at 183.628 kb on + strand at 183.628 kb on + strand at 183.628 kb on + strand at 183.629 kb on - strand at 183.629 kb on - strand at 183.868 kb on + strand, within SMa0335 at 183.868 kb on + strand, within SMa0335 at 184.242 kb on - strand, within SMa0335
Per-strain Table
Position Strand Gene LocusTag Fraction R2A control for volatile experiments remove 182,499 + SMa0329 0.46 -0.7 182,500 - SMa0329 0.47 -0.3 182,500 - SMa0329 0.47 -0.3 182,682 - SMa0329 0.70 +0.8 182,700 - SMa0329 0.73 -0.6 183,231 - SMa0333 0.23 -2.1 183,238 + SMa0333 0.26 -1.1 183,238 + SMa0333 0.26 -0.5 183,381 - +1.3 183,381 - -1.0 183,628 + -0.8 183,628 + -0.3 183,628 + +1.6 183,628 + +1.6 183,628 + +0.3 183,629 - +0.9 183,629 - -0.5 183,868 + SMa0335 0.38 +0.0 183,868 + SMa0335 0.38 -0.3 184,242 - SMa0335 0.86 -0.4
Or see this region's nucleotide sequence